scholarly journals Electron Microscopic Study of “Adenosine Triphosphatase” Activity in Malignant Melanoma Cells of the Syrian Golden Hamster**From the Division of Oncology, The Chicago Medical School, Institute for Medical Research, Chicago, Illinois.

1965 ◽  
Vol 44 (4) ◽  
pp. 264-272 ◽  
Author(s):  
Takashi Nakai
1973 ◽  
Vol 21 (5) ◽  
pp. 488-498 ◽  
Author(s):  
R. E. POELMANN ◽  
W. T. DAEMS ◽  
E. J. VAN LOHUIZEN

This cytochemical and electron microscopic study on peritoneal macrophages of the guinea pig has raised doubts concerning the validity of lead methods for the demonstration of plasma membrane-bound adenosine triphosphatase activity. The problems encountered are inherent in the use of lead ions as a capture reagent. The nonenzymatically formed precipitates reflect sites of heterogeneous nucleation specific for certain kinds of cells, e.g., resident peritoneal macrophages, eosinophilic granulocytes and, to a lesser degree, exudate monocytes. This type of precipitation is also catalyzed on the surface of nonbiologic matrices such as latex particles. Enzymatic processes may well occur, but they cannot be distinguished from nonenzymatic processes.


1988 ◽  
Vol 30 (2) ◽  
pp. 104-119
Author(s):  
Toshihiko INAGE ◽  
Yasuhiro TERANISHI ◽  
Masatoshi KOBAYASHI ◽  
Shigeaki KOIDE ◽  
Jun TAKAGI ◽  
...  

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