scholarly journals Fluorescence in situ hybridization investigation of cutaneous lesions in acute promyelocytic leukemia

2005 ◽  
Vol 18 (12) ◽  
pp. 1569-1576 ◽  
Author(s):  
Joanna E Wrede ◽  
Uma Sundram ◽  
Sabine Kohler ◽  
Athena M Cherry ◽  
Daniel A Arber ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
Promyelocytic Leukemia ◽  
Cutaneous Lesions

scholarly journals Detection of residual leukemic cells in patients with acute promyelocytic leukemia by the fluorescence in situ hybridization method: potential for predicting relapse

Blood ◽  
1995 ◽  
Vol 85 (2) ◽  
pp. 495-499 ◽  
Author(s):  
L Zhao ◽  
KS Chang ◽  
EH Estey ◽  
K Hayes ◽  
AB Deisseroth ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
Bone Marrow Cells ◽  
Residual Disease ◽  
Promyelocytic Leukemia ◽  
Leukemic Cells ◽  
Low Frequencies

Abstract The translocation between chromosomes 15 and 17, t(15;17)(q22–24;q11– 21), is present in the bone marrow cells of most patients with acute promyelocytic leukemia (APL). Although conventional cytogenetic methods are useful for diagnosing this disease, difficulties are experienced in detecting residual disease among those patients who have achieved remission. In this study, we used the fluorescence in situ hybridization (FISH) method to attempt to detect residual leukemic cells in 10 APL patients in clinical remission. The duration of remission ranged from 2 to 93 months at the time of study. Multiple bone marrow samples were analyzed by FISH in most patients. In 6 patients, no cell with t(15;17) was found. These patients remain in complete remission at present (approximately 25 to 33 months since first studied by FISH). In 4 patients, low frequencies of cells with t(15;17) were observed in at least one bone marrow sample examined. All of these patients relapsed within 1 to 14 months. No cell with t(15;17) was identified by the conventional G-banding method in any sample. The FISH results correlated well with that of a two-round nested reverse transcription polymerase chain reaction assay that was performed on the same samples. Thus, our study suggests that FISH is potentially a useful tool for detecting residual APL cells and for identifying patients at high risk of relapse.

Efficacy of Fluorescence In Situ Hybridization for Detecting PML/RARA Gene Fusion in Treated and Untreated Acute Promyelocytic Leukemia

1994 ◽  
Vol 69 (11) ◽  
pp. 1047-1053 ◽  
Author(s):  
CHRIS R. SCHAD ◽  
CURTIS A. HANSON ◽  
ELISABETH PAIETTA ◽  
JAMES CASPER ◽  
SYED M. JALAL ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
Gene Fusion ◽  
Promyelocytic Leukemia

A new variant t(6;15;17)(q25;q22;q21) in acute promyelocytic leukemia: fluorescence in situ hybridization confirmation

2005 ◽  
Vol 159 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Virginie Eclache ◽  
Franck Viguie ◽  
Claudie Frocrain ◽  
Bruno Cassinat ◽  
Christine Chomienne ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
Promyelocytic Leukemia ◽  
New Variant

scholarly journals Detection of residual leukemic cells in patients with acute promyelocytic leukemia by the fluorescence in situ hybridization method: potential for predicting relapse

Blood ◽  
1995 ◽  
Vol 85 (2) ◽  
pp. 495-499
Author(s):  
L Zhao ◽  
KS Chang ◽  
EH Estey ◽  
K Hayes ◽  
AB Deisseroth ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
Bone Marrow Cells ◽  
Residual Disease ◽  
Promyelocytic Leukemia ◽  
Leukemic Cells ◽  
Low Frequencies

The translocation between chromosomes 15 and 17, t(15;17)(q22–24;q11– 21), is present in the bone marrow cells of most patients with acute promyelocytic leukemia (APL). Although conventional cytogenetic methods are useful for diagnosing this disease, difficulties are experienced in detecting residual disease among those patients who have achieved remission. In this study, we used the fluorescence in situ hybridization (FISH) method to attempt to detect residual leukemic cells in 10 APL patients in clinical remission. The duration of remission ranged from 2 to 93 months at the time of study. Multiple bone marrow samples were analyzed by FISH in most patients. In 6 patients, no cell with t(15;17) was found. These patients remain in complete remission at present (approximately 25 to 33 months since first studied by FISH). In 4 patients, low frequencies of cells with t(15;17) were observed in at least one bone marrow sample examined. All of these patients relapsed within 1 to 14 months. No cell with t(15;17) was identified by the conventional G-banding method in any sample. The FISH results correlated well with that of a two-round nested reverse transcription polymerase chain reaction assay that was performed on the same samples. Thus, our study suggests that FISH is potentially a useful tool for detecting residual APL cells and for identifying patients at high risk of relapse.

scholarly journals A complex translocation (3;17;15) in acute promyelocytic leukemia confirmed by fluorescence in situ hybridization

2016 ◽  
Vol 12 (6) ◽  
pp. 4717-4719 ◽  
Author(s):  
Yanming Wang ◽  
Junjie Ma ◽  
Xinguang Liu ◽  
Riming Liu ◽  
Lingling Xu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
Promyelocytic Leukemia ◽  
Complex Translocation

An eight-way variant t(15;17) in acute promyelocytic leukemia elucidated using fluorescence in situ hybridization

1995 ◽  
Vol 83 (2) ◽  
pp. 136-139 ◽  
Author(s):  
Lynne R. Hiorns ◽  
G. John Swansbury ◽  
Daniel Catovsky
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
Promyelocytic Leukemia

High frequency of trisomy 8 in acute promyelocytic leukemia: A fluorescence in situ hybridization study

1997 ◽  
Vol 97 (2) ◽  
pp. 161-164 ◽  
Author(s):  
Mario Sessarego ◽  
Giuseppina Fugazza ◽  
Enrico Balleari ◽  
Roberto Bruzzone ◽  
Alberto Ballestrero ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
High Frequency ◽  
Promyelocytic Leukemia ◽  
Hybridization Study ◽  
Trisomy 8

New highly sensitive fluorescence in situ hybridization method to detect PML/RARA fusion in acute promyelocytic leukemia

2003 ◽  
Vol 145 (2) ◽  
pp. 144-151 ◽  
Author(s):  
Stephanie R. Brockman ◽  
Sarah F. Paternoster ◽  
Rhett P. Ketterling ◽  
Gordon W. Dewald
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Acute Promyelocytic Leukemia ◽  
Promyelocytic Leukemia ◽  
Hybridization Method ◽  
Highly Sensitive
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