Creation of a new breeding material for creeping clover (Trifolium repens L.) hay-pasture type using the hybridization method

The studies were carried out in the Moscow region in 2019-2021. In order to create a new breeding material for creeping hay-pasture clover, samples of the Lodian type (giganteum variety), characterized by high peduncles and longer leaf petioles, and a low content of hydrocyanic acid (0.3 mg%), were selected as parental forms. Reciprocal crossing was carried out. Hybrids F1 and F2 in the first year of life were assessed by the main economic and biological characteristics: the length of the peduncles, the number of inflorescences on the plant, fodder and seed productivity, early maturity, winter hardiness and other indicators. Based on the assessment results, the best hybrids and individual biotypes were identified.

Transcatheter closure of multiple paravalvular leaks by a transapical approach under echocardiographic guidance: A case report

Case summary A patient who underwent mechanical aortic and mitral valve replacement developed three paravalvular leaks 10 months later. We located the tracks by puncturing the apex cordis under transoesophageal echocardiography guidance alone and puncturing the femoral artery guided by fluoroscopy. Three paravalvular leaks were occluded with a hybridization method simultaneously. The patient was followed up for 24 months and maintained a good condition. Conclusion Multiple paravalvular leaks after double valve replacement can be occluded in patients by the use of different approaches under echocardiographic guidance alone.

Creation of breeding material of oil flax with high cold resistance by hybridization method

Aim. The aim of the study is to create a selection material of oilseed flax with high cold resistance by hybridization. Methods. To achieve this goal, the following methods were used: simple (single and reciprocal) and complex (backcross, triple, four- and five-component step) crossing. Promising plants for cold tolerance donors were involved in the selection process. Creations of crossbreeding combinations were created on the basis of hypotheses of multiple gene action and heterosis. Results. The results of the study are the creation of hybrids of oilseed flax with high cold resistance. Conclusions. It was found that paired crosses were less effective compared to different types of complex. The formative process of complex crosses contributes to the increase of adaptive signs and properties to hypothermia. The best donor of cold resistance was the Sideral variety. Key words: flax, cold resistance, hybridization, simple crosses, complex crosses.

Preparation of PANI Modified ZnO Composites via Different Methods: Structural, Morphological and Photocatalytic Properties

Polyaniline modified zinc oxide (PANI-ZnO) photocatalyst composites were synthesized by focusing on dissolution disadvantage of ZnO. In-situ chemical oxidation polymerization method was performed under neutral conditions (PANI-ES) whereas in hybridization method physical blending was applied using emeraldine base of polyaniline (PANI-EB). PANI-ZnO composites were prepared in various ratios of aniline (ANI) to ZnO as 1%, 3%, 6% and 9%. The alterations on the structural and morphological properties of PANI-ZnO composites were compared by Fourier Transform Infrared (FT-IR), Raman Spectroscopy, X-Ray Diffraction (XRD) and Scanning Electron Microscopy-Energy Dispersive X-ray Analysis Unit (SEM-EDAX) techniques. FT-IR and Raman spectroscopy confirmed the presence of PANI in all composites. SEM images revealed the morphological differences of PANI-ZnO composites based on PANI presence and preparation methods. Photocatalytic performances of PANI-ZnO specimens were investigated by following the degradation of methylene blue (MB) in aqueous medium under UVA irradiation. The effects of catalyst dose and initial dye concentration were also studied. MB degradation was followed by both decolorization extents and removal of aromatic fractions. PANI-ZnO composites expressed enhanced photocatalytic performance (~95% for both methods) as compared to sole ZnO (~87%). The hybridization method was found to be more efficient than the in-situ chemical oxidation polymerization method emphasizing the significance of the neutral medium.

A Novel Dynamic Hybridization Method for Best Feature Selection

Hybrid metaheuristics has received a lot of attention lately to solve combinatorial optimization problems. The purpose of hybridization is to create a cooperation between metaheuristics for better solutions. Most proposed works were interested in static hybridization. The objective of this work is to propose a novel dynamic hybridization method (GPBD) that generates the most suitable sequential hybridization between GA, PSO, BAT, and DE metaheuristics, according to each problem. The authors choose to test this approach for solving the best feature selection problem in a wrapper tactic, performed on face image recognition datasets, with the k-nearest neighbor (KNN) learning algorithm. The comparative study of the metaheuristics and their hybridization GPBD shows that the proposed approach achieved the best results. It was definitely competitive with other filter approaches proposed in the literature. It achieved a perfect accuracy score of 100% for Orl10P, Pix10P, and PIE10P datasets.

Ultrasensitive hybridization capture: Reliable detection of <1 copy/mL short cell-free DNA from large-volume urine samples

Urine cell-free DNA (cfDNA) is a valuable non-invasive biomarker with broad potential clinical applications, but there is no consensus on its optimal pre-analytical methodology, including the DNA extraction step. Due to its short length (majority of fragments <100 bp) and low concentration (ng/mL), urine cfDNA is not efficiently recovered by conventional silica-based extraction methods. To maximize sensitivity of urine cfDNA assays, we developed an ultrasensitive hybridization method that uses sequence-specific oligonucleotide capture probes immobilized on magnetic beads to improve extraction of short cfDNA from large-volume urine samples. Our hybridization method recovers near 100% (95% CI: 82.6–117.6%) of target-specific DNA from 10 mL urine, independent of fragment length (25–150 bp), and has a limit of detection of ≤5 copies of double-stranded DNA (0.5 copies/mL). Pairing hybridization with an ultrashort qPCR design, we can efficiently capture and amplify fragments as short as 25 bp. Our method enables amplification of cfDNA from 10 mL urine in a single qPCR well, tolerates variation in sample composition, and effectively removes non-target DNA. Our hybridization protocol improves upon both existing silica-based urine cfDNA extraction methods and previous hybridization-based sample preparation protocols. Two key innovations contribute to the strong performance of our method: a two-probe system enabling recovery of both strands of double-stranded DNA and dual biotinylated capture probes, which ensure consistent, high recovery by facilitating optimal probe density on the bead surface, improving thermostability of the probe-bead linkage, and eliminating interference by endogenous biotin. We originally designed the hybridization method for tuberculosis diagnosis from urine cfDNA, but expect that it will be versatile across urine cfDNA targets, and may be useful for other cfDNA sample types and applications beyond cfDNA. To make our hybridization method accessible to new users, we present a detailed protocol and straightforward guidelines for designing new capture probes.