The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

2006 ◽  
Vol 1 (2) ◽  
pp. 581-585 ◽  
Author(s):  
Piotr Chomczynski ◽  
Nicoletta Sacchi
Keyword(s):  
Rna Isolation ◽  
Single Step ◽  
Step Method ◽  
Chloroform Extraction ◽  
Guanidinium Thiocyanate

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 ◽  
Vol 162 (1) ◽  
pp. 156-159 ◽  
Author(s):  
Piotr Chomczynski ◽  
Nicoletta Sacchi
Keyword(s):  
Rna Isolation ◽  
Single Step ◽  
Step Method ◽  
Chloroform Extraction ◽  
Guanidinium Thiocyanate

Single-Step Method of Total RNA Isolation by Guanidine-Phenol Extraction

2013 ◽  
Author(s):  
Piotr Chomczynski ◽  
William Wilfinger ◽  
Karol Mackey
Keyword(s):  
Rna Isolation ◽  
Single Step ◽  
Step Method ◽  
Total Rna ◽  
Phenol Extraction ◽  
Total Rna Isolation

Substitution of Chloroform by Bromochloropropane in the Single-Step Method of RNA Isolation

1995 ◽  
Vol 225 (1) ◽  
pp. 163-164 ◽  
Author(s):  
P. Chomczynski ◽  
K. Mackey
Keyword(s):  
Rna Isolation ◽  
Single Step ◽  
Step Method

Single-Step Method of Total RNA Isolation by Sodium Dodecyl Sulfate/Phenol Extraction from Cultured Cells

1997 ◽  
Vol 251 (2) ◽  
pp. 291-292 ◽  
Author(s):  
Jubao Duan ◽  
Xin Cai ◽  
Limei Zhou ◽  
Jiaxi Wang
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Cultured Cells ◽  
Sodium Dodecyl ◽  
Rna Isolation ◽  
Single Step ◽  
Step Method ◽  
Total Rna ◽  
Phenol Extraction ◽  
Dodecyl Sulfate ◽  
Total Rna Isolation

Single-Step Method of Total RNA Isolation by Guanidine-Phenol Extraction

2017 ◽  
pp. 1-6
Author(s):  
Piotr Chomczynski ◽  
William Wilfinger ◽  
Karol Mackey
Keyword(s):  
Rna Isolation ◽  
Single Step ◽  
Step Method ◽  
Total Rna ◽  
Phenol Extraction ◽  
Total Rna Isolation

scholarly journals A single-step method for RNA isolation from tropical crops in the field

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
J.-C. Breitler ◽  
C. Campa ◽  
F. Georget ◽  
B. Bertrand ◽  
H. Etienne
Keyword(s):  
Rna Isolation ◽  
Single Step ◽  
Step Method ◽  
Tropical Crops

Purification of Urokinase from Complex Mixtures Using Immobilized Monoclonal Antibody Against Urokinase Light Chain

1983 ◽  
Vol 49 (01) ◽  
pp. 024-027 ◽  
Author(s):  
David Vetterlein ◽  
Gary J Calton
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Culture Media ◽  
Biological Fluids ◽  
Single Step ◽  
High Yield ◽  
Step Method ◽  
Commercial Preparation ◽  
E Coli ◽  
Tissue Culture Media

SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

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