scholarly journals Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials

2014 ◽  
Vol 47 (2) ◽  
pp. 220-225 ◽  
Author(s):  
Yu Hoshino ◽  
Yuka Arata ◽  
Yusuke Yonamine ◽  
Shih-Hui Lee ◽  
Aki Yamasaki ◽  
...  
Keyword(s):  
Affinity Separation ◽  
Gel Beads ◽  
Separation Of Proteins

Affinity Separation of Proteins in Aqueous Two-Phase Systems

1990 ◽  
pp. 335-344 ◽  
Author(s):  
T. Franco ◽  
B. A. Andrews ◽  
O. Cascone ◽  
C. Hodgson ◽  
A. T. Andrews ◽  
...  
Keyword(s):  
Affinity Separation ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Separation Of Proteins

Affinity separation of proteins in aqueous three-phase systems

1988 ◽  
Vol 175 (1) ◽  
pp. 154-161 ◽  
Author(s):  
Per-Åke Albertsson ◽  
Gerd Birkenmeier
Keyword(s):  
Affinity Separation ◽  
Three Phase ◽  
Phase Systems ◽  
Separation Of Proteins

Synthesis of petal-like ferric oxide/cysteine architectures and their application in affinity separation of proteins

2014 ◽  
Vol 34 ◽  
pp. 468-473 ◽  
Author(s):  
Xueyan Zou ◽  
Kun Li ◽  
Yanbin Yin ◽  
Yanbao Zhao ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Ferric Oxide ◽  
Affinity Separation ◽  
Separation Of Proteins

Separation of proteins in a multicompartment electrolyzer with chambers defined by a bed of gel beads

2003 ◽  
Vol 24 (4) ◽  
pp. 577-581 ◽  
Author(s):  
Marina Cretich ◽  
Giovanna Pirri ◽  
Giacomo Carrea ◽  
Marcella Chiari
Keyword(s):  
Gel Beads ◽  
Separation Of Proteins

scholarly journals Functionalized Nano-adsorbent for Affinity Separation of Proteins

2018 ◽  
Vol 13 (1) ◽  
Author(s):  
Xueyan Zou ◽  
Fengbo Yang ◽  
Xin Sun ◽  
Mingming Qin ◽  
Yanbao Zhao ◽  
...  
Keyword(s):  
Affinity Separation ◽  
Separation Of Proteins ◽  
Nano Adsorbent

Polymer Nanoparticles Covered with Phosphorylcholine Groups and Immobilized with Antibody for High-Affinity Separation of Proteins

2008 ◽  
Vol 9 (3) ◽  
pp. 828-833 ◽  
Author(s):  
Yusuke Goto ◽  
Ryosuke Matsuno ◽  
Tomohiro Konno ◽  
Madoka Takai ◽  
Kazuhiko Ishihara
Keyword(s):  
Polymer Nanoparticles ◽  
Affinity Separation ◽  
High Affinity ◽  
Separation Of Proteins

Dye–ligand and metal chelate poly(2-hydroxyethylmethacrylate) membranes for affinity separation of proteins

1998 ◽  
Vol 799 (1-2) ◽  
pp. 83-91 ◽  
Author(s):  
M.Yakup Arıca ◽  
H.Nur Testereci ◽  
Adil Denizli
Keyword(s):  
Metal Chelate ◽  
Affinity Separation ◽  
Separation Of Proteins

On the Preparation of Bovine α-Thrombin

1978 ◽  
Vol 39 (01) ◽  
pp. 193-200 ◽  
Author(s):  
Erwin F Workman ◽  
Roger L Lundblad
Keyword(s):  
Immobilized Enzyme ◽  
Catalytic Properties ◽  
Specific Activity ◽  
Guanidine Hydrochloride ◽  
Low Molecular Weight ◽  
Reversible Process ◽  
Gel Beads ◽  
Tissue Thromboplastin ◽  
C 50 ◽  
Hydrolysis Of

SummaryAn improved method for the preparation of bovine α-thrombin is described. The procedure involves the activation of partially purified prothrombin with tissue thromboplastin followed by chromatography on Sulfopropyl-Sephadex C-50. The purified enzyme is homogeneous on polyacrylamide discontinuous gel electrophoresis and has a specific activity toward fibrinogen of 2,200–2,700 N.I.H. U/mg. Its stability on storage in liquid media is dependent on both ionic strenght and temperature. Increasing ionic strength and decreasing temperature result in optimal stability. The denaturation of α-thrombin by guanidine hydrochloride was found to be a partially reversible process with the renatured species possessing properties similar to “aged” thrombin. In addition, the catalytic properties of a-thrombin covalently attached to agarose gel beads were also examined. The activity of the immobilized enzyme toward fibrinogen was affected to a much greater extent than was the hydrolysis of low molecular weight, synthetic substrates.

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