scholarly journals Decreased guanine nucleotide exchange factor activity in eIF2B-mutated patients

2004 ◽  
Vol 12 (7) ◽  
pp. 561-566 ◽  
Author(s):  
Anne Fogli ◽  
Raphael Schiffmann ◽  
Lynne Hugendubler ◽  
Patricia Combes ◽  
Enrico Bertini ◽  
...  
Keyword(s):  
Guanine Nucleotide Exchange Factor ◽  
Guanine Nucleotide ◽  
Nucleotide Exchange ◽  
Factor Activity ◽  
Exchange Factor ◽  
Guanine Nucleotide Exchange ◽  
Nucleotide Exchange Factor

scholarly journals A Burkholderia pseudomallei Type III Secreted Protein, BopE, Facilitates Bacterial Invasion of Epithelial Cells and Exhibits Guanine Nucleotide Exchange Factor Activity

2003 ◽  
Vol 185 (16) ◽  
pp. 4992-4996 ◽  
Author(s):  
Mark P. Stevens ◽  
Andrea Friebel ◽  
Lowrie A. Taylor ◽  
Michael W. Wood ◽  
Philip J. Brown ◽  
...  
Keyword(s):  
Burkholderia Pseudomallei ◽  
Guanine Nucleotide Exchange Factor ◽  
Secreted Protein ◽  
Guanine Nucleotide ◽  
Nucleotide Exchange ◽  
Factor Activity ◽  
Type Iii ◽  
Exchange Factor ◽  
Guanine Nucleotide Exchange ◽  
Nucleotide Exchange Factor

ABSTRACT We report the characterization of BopE, a type III secreted protein that is encoded adjacent to the Burkholderia pseudomallei bsa locus and is homologous to Salmonella enterica SopE/SopE2. Inactivation of bopE impaired bacterial entry into HeLa cells, indicating that BopE facilitates invasion. Consistent with this notion, BopE expressed in eukaryotic cells induced rearrangements in the subcortical actin cytoskeleton, and purified BopE exhibited guanine nucleotide exchange factor activity for Cdc42 and Rac1 in vitro.

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