Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip

Lab on a Chip ◽  
2008 ◽  
Vol 8 (4) ◽  
pp. 602 ◽  
Author(s):  
Louis P. Hromada ◽  
Brian J. Nablo ◽  
John J. Kasianowicz ◽  
Michael A. Gaitan ◽  
Don L. DeVoe
Keyword(s):  
Ion Channels ◽  
Single Molecule ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Bilayer Lipid ◽  
Membrane Bound

Microfluidic Emulsion Generation and Trapping Enabling Droplet-Interfaced Bilayer Lipid Membrane Arrays

2009 ◽  
Author(s):  
C. Shao ◽  
D. L. DeVoe
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Protein Interactions ◽  
Lipid Membranes ◽  
High Throughput Screening ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Single Molecule Level ◽  
Bilayer Lipid ◽  
Ion Translocation

Freestanding bilayer lipid membranes provide an exceptional platform for measurements of lipid/protein interactions and ion translocation events at the single molecule level. For drug screening applications, large arrays of individual bilayer supports are required. However, an effective method for generating, stabilizing, and monitoring arrays of lipid bilayers remains elusive. Here we investigate a novel approach towards the facile generation of bilayer arrays for high throughput screening. The approach takes advantage of fundamental microfluidic capabilities by combining an emulsion generator with droplet-interfaced membrane formation, allowing for fully-automated production of membrane arrays whose density is, in principle, unlimited.

Gating of an organic transistor through a bilayer lipid membrane with ion channels

2006 ◽  
Vol 89 (5) ◽  
pp. 053505 ◽  
Author(s):  
Daniel A. Bernards ◽  
George G. Malliaras ◽  
Gilman E. S. Toombes ◽  
Sol M. Gruner
Keyword(s):  
Ion Channels ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Bilayer Lipid ◽  
Organic Transistor

Capacitance extraction method for a free-standing bilayer lipid membrane formed over an aperture in a nanofabricated silicon chip

2020 ◽  
Vol 59 (SI) ◽  
pp. SIIK02
Author(s):  
Yasutaka Tomioka ◽  
Shogo Takashima ◽  
Masataka Moriya ◽  
Hiroshi Shimada ◽  
Fumihiko Hirose ◽  
...  
Keyword(s):  
Extraction Method ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Free Standing ◽  
Capacitance Extraction ◽  
Bilayer Lipid
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