Hybridization kinetics of double-stranded DNA probes for rapid molecular analysis

The Analyst ◽  
2009 ◽  
Vol 134 (8) ◽  
pp. 1675 ◽  
Author(s):  
Vinay Gidwani ◽  
Reza Riahi ◽  
Donna D. Zhang ◽  
Pak Kin Wong
Keyword(s):  
Molecular Analysis ◽  
Dna Probes ◽  
Double Stranded Dna ◽  
Kinetics Of

scholarly journals Detailed Alignment of Saccharum and Sorghum Chromosomes: Comparative Organization of Closely Related Diploid and Polyploid Genomes

Genetics ◽  
1998 ◽  
Vol 150 (4) ◽  
pp. 1663-1682 ◽  
Author(s):  
R Ming ◽  
S-C Liu ◽  
Y-R Lin ◽  
J da Silva ◽  
W Wilson ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Saccharum Officinarum ◽  
Dna Probes ◽  
Genetic Maps ◽  
Linkage Groups ◽  
Structural Polymorphism ◽  
Sorghum Genome ◽  
F1 Progeny ◽  
Duplicated Loci ◽  
Intrachromosomal Rearrangements

Abstract The complex polyploid genomes of three Saccharum species have been aligned with the compact diploid genome of Sorghum (2n = 2x = 20). A set of 428 DNA probes from different Poaceae (grasses) detected 2460 loci in F1 progeny of the crosses Saccharum officinarum Green German × S. spontaneum IND 81-146, and S. spontaneum PIN 84-1 × S. officinarum Muntok Java. Thirty-one DNA probes detected 226 loci in S. officinarum LA Purple × S. robustum Molokai 5829. Genetic maps of the six Saccharum genotypes, including up to 72 linkage groups, were assembled into “homologous groups” based on parallel arrangements of duplicated loci. About 84% of the loci mapped by 242 common probes were homologous between Saccharum and Sorghum. Only one interchromosomal and two intrachromosomal rearrangements differentiated both S. officinarum and S. spontaneum from Sorghum, but 11 additional cases of chromosome structural polymorphism were found within Saccharum. Diploidization was advanced in S. robustum, incipient in S. officinarum, and absent in S. spontaneum, consistent with biogeographic data suggesting that S. robustum is the ancestor of S. officinarum, but raising new questions about the antiquity of S. spontaneum. The densely mapped Sorghum genome will be a valuable tool in ongoing molecular analysis of the complex Saccharum genome.

Kinetics of collapse and decollapse of a single double-stranded DNA chain

1996 ◽  
Vol 106 (1) ◽  
pp. 367-378 ◽  
Author(s):  
K. Yoshikawa
Keyword(s):  
Double Stranded Dna ◽  
Dna Chain ◽  
Kinetics Of

Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes

1989 ◽  
Vol 3 (4) ◽  
pp. 363-373 ◽  
Author(s):  
Wasun Chantratita ◽  
Erik A. Henchal ◽  
Chalobon Yoosook
Keyword(s):  
In Situ Hybridization ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Rapid Detection ◽  
Dna Probes ◽  
Double Stranded Dna ◽  
Simplex Virus

scholarly journals Molecular Analysis of Neutrophil Differentiation from Human Induced Pluripotent Stem Cells Delineates the Kinetics of Key Regulators of Hematopoiesis

Stem Cells ◽  
2016 ◽  
Vol 34 (6) ◽  
pp. 1513-1526 ◽  
Author(s):  
Colin L. Sweeney ◽  
Ruifeng Teng ◽  
Hongmei Wang ◽  
Randall K. Merling ◽  
Janet Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Molecular Analysis ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Neutrophil Differentiation ◽  
Kinetics Of

scholarly journals Stoichiometry and kinetics of complex formation by the recA protein and a double-stranded DNA

FEBS Letters ◽  
1982 ◽  
Vol 145 (1) ◽  
pp. 53-56 ◽  
Author(s):  
A.A. Volodin ◽  
V.A. Shepelev ◽  
Yu.N. Kosaganov
Keyword(s):  
Complex Formation ◽  
Reca Protein ◽  
Double Stranded Dna ◽  
Kinetics Of

scholarly journals Hybridization kinetics between immobilized double-stranded DNA probes and targets containing embedded recognition segments

2011 ◽  
Vol 39 (15) ◽  
pp. e99-e99 ◽  
Author(s):  
Bryan A. Baker ◽  
Valeria T. Milam
Keyword(s):  
Dna Probes ◽  
Double Stranded Dna

Hybridization Reaction Kinetics of DNA Probes on Beads Arrayed in a Capillary Enhanced by Turbulent Flow

2003 ◽  
Vol 75 (13) ◽  
pp. 3079-3085 ◽  
Author(s):  
Yoshinobu Kohara
Keyword(s):  
Turbulent Flow ◽  
Reaction Kinetics ◽  
Dna Probes ◽  
Kinetics Of ◽  
Hybridization Reaction
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