Copper modulates the large dense core vesicle secretory pathway in PC12 cells

Metallomics ◽  
2013 ◽  
Vol 5 (6) ◽  
pp. 700 ◽  
Author(s):  
Clare Duncan ◽  
Laura Bica ◽  
Peter J. Crouch ◽  
Aphrodite Caragounis ◽  
Grace E. Lidgerwood ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Secretory Pathway ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Large Dense Core Vesicle

Large Dense-Core Vesicle Exocytosis in PC12 Cells

Methods ◽  
1998 ◽  
Vol 16 (2) ◽  
pp. 204-208 ◽  
Author(s):  
Vadim A. Klenchin ◽  
Judith A. Kowalchyk ◽  
Thomas F.J. Martin
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals Atg16L1, an essential factor for canonical autophagy, participates in hormone secretion from PC12 cells independently of autophagic activity

2012 ◽  
Vol 23 (16) ◽  
pp. 3193-3202 ◽  
Author(s):  
Koutaro Ishibashi ◽  
Takefumi Uemura ◽  
Satoshi Waguri ◽  
Mitsunori Fukuda
Keyword(s):  
Pc12 Cells ◽  
Secretory Pathway ◽  
Biological Activities ◽  
Hormone Secretion ◽  
Cell Types ◽  
Small Gtpase ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Dense Core Vesicles ◽  
Autophagic Activity

Autophagy is a bulk degradation system in all eukaryotic cells and regulates a variety of biological activities in higher eukaryotes. Recently involvement of autophagy in the regulation of the secretory pathway has also been reported, but the molecular mechanism linking autophagy with the secretory pathway remains largely unknown. Here we show that Atg16L1, an essential protein for canonical autophagy, is localized on hormone-containing dense-core vesicles in neuroendocrine PC12 cells and that knockdown of Atg16L1 causes a dramatic reduction in the level of hormone secretion independently of autophagic activity. We also find that Atg16L1 interacts with the small GTPase Rab33A and that this interaction is required for the dense-core vesicle localization of Atg16L1 in PC12 cells. Our findings indicate that Atg16L1 regulates not only autophagy in all cell types, but also secretion from dense-core vesicles, presumably by acting as a Rab33A effector, in particular cell types.

scholarly journals Role of the polybasic sequence in the Doc2α C2B domain in dense-core vesicle exocytosis in PC12 cells

2010 ◽  
pp. no-no ◽  
Author(s):  
Mai Sato ◽  
Yasunori Mori ◽  
Takahide Matsui ◽  
Ryo Aoki ◽  
Manami Oya ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

Synaptotagmin function in dense core vesicle exocytosis studied in cracked PC12 cells

10.1038/nn869 ◽  
2002 ◽  
Vol 5 (7) ◽  
pp. 649-656 ◽  
Author(s):  
Ok-Ho Shin ◽  
Josep Rizo ◽  
Thomas C. Südhof
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis
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