A fast high performance liquid chromatographic (HPLC) analysis of amino acid phenylketonuria disorder in dried blood spots and serum samples, employing C18 monolithic silica columns and photo diode array detection

2015 ◽  
Vol 7 (18) ◽  
pp. 7560-7567 ◽  
Author(s):  
Farideh Haghighi ◽  
Zahra Talebpour ◽  
Vali Amini ◽  
Amir Ahmadzadeh ◽  
Mohsen Farhadpour
Keyword(s):  
High Performance ◽  
Hplc Analysis ◽  
Dried Blood Spots ◽  
High Performance Liquid Chromatographic ◽  
Diode Array ◽  
Diode Array Detection ◽  
Serum Samples ◽  
Blood Spots ◽  
Array Detection ◽  
Liquid Chromatographic

A gradient HPLC-PDA method applying a monolithic RP-C18 column for phenylalanine and tyrosine quantization in dried blood spots, within 6 min.

Fingerprint Analysis of Polygonatum odoratum (Mill.) Druce Using High-Performance Liquid Chromatographic Method with Diode-Array Detection

2013 ◽  
Vol 781-784 ◽  
pp. 1199-1202
Author(s):  
Guang Sheng Xi
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Diode Array ◽  
Diode Array Detection ◽  
Fingerprint Analysis ◽  
Line Identification ◽  
Liquid Chromatographic Method ◽  
Average Similarity ◽  
Array Detection ◽  
Liquid Chromatographic

The fingerprints analysis of different lines ofPolygonatum odoratum(Mill.) Druce were established by high-performance liquid chromatographic with diode-array detection (HPLC/DAD). The analyses were performed on ZORBAX Eclipse XDB-C18(4.6×250 mm, 5 μm) column using a mobile phase (aqueous 0.05% formic acid-acetonitrile) under gradient conditions. The flow rate was 1.0 ml/min. The detecting wavelength was set at 210 nm. HPLC fingerprints of two lines were little different. The similarity of the same line was greater than 0.9, and the average similarity of the different lines was 0.794. This method could be suitable for the line identification and quality control ofPolygonatum odoratum(Mill.) Druce.

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