scholarly journals Water-mediated influence of a crowded environment on internal vibrations of a protein molecule

2016 ◽  
Vol 18 (6) ◽  
pp. 4881-4890 ◽  
Author(s):  
Anna Kuffel ◽  
Jan Zielkiewicz
Keyword(s):  
Protein Molecule ◽  
Protein Molecules ◽  
Single Protein ◽  
Crowded Environment ◽  
Internal Vibrations

The influence of crowding on the protein inner dynamics is examined by putting a single protein molecule close to one or two neighboring protein molecules.

scholarly journals Blinking fluorophores: what do they tell us about protein dynamics?

2006 ◽  
Vol 34 (5) ◽  
pp. 979-982 ◽  
Author(s):  
C.R. Bagshaw ◽  
D. Cherny
Keyword(s):  
Protein Dynamics ◽  
Dynamic Properties ◽  
Protein Molecule ◽  
Emission Rates ◽  
Dynamic Heterogeneity ◽  
Similar Time ◽  
Emission Signal ◽  
Protein Molecules ◽  
Single Protein ◽  
Low Activity

The ability to detect emission from a single fluorophore presents a powerful tool to probe the dynamic properties of protein molecules during their interactions with ligands. Here, different classes of experiments are reviewed and a distinction is drawn between experiments that monitor signals from a large number of proteins, one molecule at a time, from those that follow a single protein molecule over many individual cycles. The latter approach is potentially capable of resolving dynamic heterogeneity, such as that displayed by enzymes that fluctuate between high and low activity states. Other factors that need to be considered are the origin of the fluctuations in the emission signal and the extent to which this represents the properties of the protein under investigation, as opposed to the probe itself. Most fluorophores show fluctuations in their emission rates, termed flickering, blinking or intermittency, which may occur on a similar time-scale as the event under investigation.

scholarly journals Highly Sensitive Protein Quantification by Direct Kinetic Fingerprinting of Single Protein Molecules

2021 ◽  
Vol 120 (3) ◽  
pp. 185a
Author(s):  
Tanmay Chatterjee ◽  
Achim Knappik ◽  
Erin Sandford ◽  
Muneesh Tewari ◽  
Sung Won Choi ◽  
...  
Keyword(s):  
Protein Quantification ◽  
Highly Sensitive ◽  
Protein Molecules ◽  
Single Protein

Measurement of Single Protein Molecules Using Digital ELISA

2013 ◽  
pp. 223-242 ◽  
Author(s):  
David M. Rissin ◽  
David H. Wilson ◽  
David C. Duffy
Keyword(s):  
Protein Molecules ◽  
Single Protein ◽  
Digital Elisa

Measuring the force of single protein molecule detachment from surfaces with AFM

2010 ◽  
Vol 75 (1) ◽  
pp. 252-259 ◽  
Author(s):  
Theodora S. Tsapikouni ◽  
Yannis F. Missirlis
Keyword(s):  
Protein Molecule ◽  
Single Protein

Selective transport of single protein molecules inside gold nanotubes

2012 ◽  
Vol 1238 ◽  
pp. 11-14 ◽  
Author(s):  
Changbei Ma ◽  
Rui Han ◽  
Shengda Qi ◽  
Edward S. Yeung
Keyword(s):  
Selective Transport ◽  
Protein Molecules ◽  
Single Protein

scholarly journals Dynamical Observations of Brownian Motions on Single Protein Molecules Using Diffracted Electron Tracking (DET)

2010 ◽  
Vol 98 (3) ◽  
pp. 589a
Author(s):  
Yuji C. Sasaki ◽  
Hiroshi Sekiguchi ◽  
Akihiro Ohsaki ◽  
Yasuhisa Hirohata ◽  
Akira Ishikawa
Keyword(s):  
Brownian Motions ◽  
Protein Molecules ◽  
Single Protein

Efficient nanoplasmonic antennas for fabricating single protein molecule detector

2015 ◽  
Author(s):  
Sharmistha Chatterjee ◽  
Venkata Ramanaiah Dantham ◽  
Sahid Hussain
Keyword(s):  
Protein Molecule ◽  
Single Protein
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