Effect of six-week traditional resistance and functional training on functional performance in female netball players

Background Improving the quality of functional movements in athletes generally requires additional training targeting specific functional deficiencies. However, well-rounded, traditional strength and conditioning program should also improve player’s movement quality. Therefore, the primary aim of this study was to compare the effect of two different six-week interventions on the functional score of female netball players. Methods In a randomized controlled study, players were divided into control and intervention group. Both groups completed identical six-week strength and conditioning program, with the intervention group also completing additional corrective exercises three sessions per week during the same period. Results The FMS® score was significantly higher in the intervention group after 6-week program (f = 9.85, p = 0.004). However, the differences in total score may be attributed mainly to differences between groups in active straight leg raise (p = 0.004) and trunk stability push-up test (p = 0.02), as other individual tests demonstrated similar time and group effect. Conclusion These results indicate that although FMS® based intervention may improve overall functional movement score, the athletes in both groups have demonstrated similar improvements in most of the individual tests. Hence, a well-rounded strength and conditioning program incorporating athlete-specific exercises based on limitations identified in the functional movement screen, may result in a balanced training strategy and reduce the need for supplementary functional training sessions.

Two modes of evolution shape bacterial strain diversity in the gut for thousands of generations

How and at what pace bacteria evolve when colonizing healthy hosts remains unclear. Here, by monitoring evolution for more than six thousand generations in the mouse gut, we show that the successful colonization of an invader Escherichia coli depends on the diversity of the existing microbiota and the presence of a closely related strain. Following colonization, two modes of evolution were observed: one in which diversifying selection leads to long-term coexistence of ecotypes and a second in which directional selection propels selective sweeps. These modes can be quantitatively distinguished by the statistics of mutation trajectories. In our experiments, diversifying selection was marked by the emergence of metabolic mutations, and directional selection by acquisition of prophages, which bring their own benefits and costs. In both modes, we observed parallel evolution, with mutation accumulation rates comparable to those typically observed in vitro on similar time scales. Our results show that gut environments can rapidly generate diversifying selection and ecotype formation.

Development of a Computational Model for Investigation of and Oscillating Water Column Device with a Savonius Turbine

The present work aims to develop a computational model investigating turbulent flows in a problem that simulates an oscillating water column device (OWC) considering a Savonius turbine in the air duct region. Incompressible, two-dimensional, unsteady, and turbulent flows were considered for three different configurations: (1) free turbine inserted in a long and large channel for verification/validation of the model, (2) an enclosure domain that mimics an OWC device with a constant velocity at its inlet, and (3) the same domain as that in Case 2 with sinusoidal velocity imposed at the inlet. A dynamic rotational mesh in the turbine region was imposed. Time-averaged equations of the conservation of mass and balance of momentum with the k–ω Shear Stress Transport (SST) model for turbulence closure were solved with the finite volume method. The developed model led to promising results, predicting similar time–spatial-averaged power coefficients (CP¯) as those obtained in the literature for different magnitudes of the tip speed ratio (0.75 ≤ λ ≤ 2.00). The simulation of the enclosure domain increased CP¯ for all studied values of λ in comparison with a free turbine (Case 1). The imposition of sinusoidal velocity (Case 3) led to a similar performance as that obtained for constant velocity (Case 2).

Adequate Immunogenicity And Low Rate of Severe Adverse Events After SARS-CoV-2 mRNA-Based Vaccination In Patients With Solid Malignancies On Active Treatment Starts To Decline 3 Months After Complete Primary Course of Vaccination

Background: SARS-CoV-2 vaccination in cancer patients is crucial since they are at increased risk of severe COVID-19 disease course, but data on efficacy and safety of vaccination are scarce.Methods: We performed a prospective observational study of patients with solid cancers on active anticancer treatment (chemotherapy, immunotherapy with immune checkpoint inhibitors (ICI) or targeted therapy) that received mRNA-based SARS-CoV-2 vaccination at two institutions in Slovenia. The immunogenicity was assessed by the detection of anti-SARS-CoV-2 S1 IgG antibodies in serum; patients were sampled before, 2-3 weeks after the first dose, 2-3 weeks after the second dose, and 3 months after the complete primary course of vaccination. The results were also compared to controls, sampled at similar time points.Results: Between March and July 2021 112 patients were included in the analysis. The seroconversion rate in patients without prior COVID-19 infection was 96% after the complete primary course of vaccination with 2 doses, compared to 100% for healthy controls. The seroconversion rate after vaccination for patients on chemotherapy, ICI, and targeted therapy was 100%, 91%, and 97%, respectively. All controls and the majority of patients on chemotherapy and targeted therapy, but only 83% for patients on ICI were adequate responders (anti-SARS-CoV-2 S1 IgG ≥ 880 ng/ml). Three months after the vaccination, a significant drop in antibody levels was observed in patients receiving ICI compared to controls (P < 0.001). Adverse events were mostly mild and predictable, none of the patients experienced serious adverse events after vaccination.Conclusions: Immunogenicity after mRNA-based vaccination against SARS-CoV-2 in cancer patients is only slightly impaired, but influenced by the type of anticancer therapy received. Patients on ICI have the slightest and gradual antibody production. Since antibody levels decline after three months, a third vaccination dose is reasonable to provide adequate protection against severe COVID-19 disease course.The study was approved by the National Ethics Committee (No. 0120-39/2021/6)

Reuse of Pruning Waste from Subtropical Fruit Trees and Urban Gardens as a Source of Nutrients: Changes in the Physical, Chemical, and Biological Properties of the Soil

A field experiment was conducted on the Andalusian coast (Granada, Southern Spain) to study the time course of nutrient release into the soil after the addition of bagged pruning waste from subtropical orchard trees (avocado, cherimoya, and mango) and urban garden waste over three two-year periods. N, P, and K concentrations were greater in the garden waste, whilst avocado and cherimoya pruning waste registered the highest values for Mg. In general, micronutrient contents were low in all waste, especially Cu. Macronutrient release followed a three-phase dynamic: fast initial release, intermediate stabilization, and final increase. Garden waste showed a similar time course in all three trees and released greater concentrations of K and P. The annual decomposition rate factor k was negative for N and Ca in the avocado tree, indicating strong biological activity in this plot. Avocado, cherimoya, and garden waste showed a good microbial degradation, improving soil quality by increasing carbon and nitrogen contents as well as soil microbial activity. As for the mango tree, its special microclimatic conditions appeared to favor waste photodegradation, thus eliminating nutrients that were not incorporated into the soil. Soil enzymatic activities increased in the avocado and cherimoya trees with the addition of all waste. In the mango tree, only an increase in urease was detected after the addition of garden waste. Our results suggest that the time course of organic waste in different subtropical trees grown on similar soils is significantly conditioned by the microclimatic characteristics.

Regulation of KHNYN antiviral activity by the extended di-KH domain and nucleo-cytoplasmic trafficking

The zinc finger antiviral protein (ZAP) restricts a broad range of viruses by binding CpG dinucleotides in viral RNA to target it for degradation and inhibit its translation. KHNYN was recently identified as an antiviral protein required for ZAP to inhibit retroviral replication, though little is known about its functional determinants. KHNYN contains an N-terminal extended di-KH-like domain, a PIN endoribonuclease domain and a C-terminal CUBAN domain that binds NEDD8 and ubiquitin. We show that deletion of the extended di-KH domain reduces its antiviral activity. However, despite its similarity to RNA binding KH domains, the extended di-KH domain in KHNYN does not appear to bind RNA. Mutation of residues in the CUBAN domain that bind NEDD8 increase KHNYN abundance but do not alter its antiviral activity, suggesting that this interaction regulates KHNYN homeostatic turnover. In contrast, a CRM1-dependent nuclear export signal (NES) at the C-terminus of the CUBAN domain is required for antiviral activity. Deletion of this signal retains KHNYN in the nucleus and inhibits its interaction with ZAP. Interestingly, this NES appeared in the KHNYN lineage at a similar time as when ZAP evolved in tetrapods, indicating that these proteins may have co-evolved to restrict viral replication.

Efficacy of visual encounter surveys for coastal tailed frog detection

Coastal tailed frogs Ascaphus truei inhabit montane streams and forested habitats in the Coast and Cascade Mountains from northern California, USA, to the Skeena River watershed in northwestern British Columbia (BC), Canada. Terrestrial adults and juveniles of this cryptic biphasic species are difficult to survey as they are small, do not vocalize, and may be associated with woody ground structures or subsurface refugia at considerable distances from natal streams. We performed a comparative analysis of the detection rate of post-metamorphic coastal tailed frogs and ecological factors hypothesized to influence detection when conducting visual encounter and pitfall trap surveys. We conducted concurrent surveys in northwestern BC at six sites over similar time periods using both techniques. The average detection rate of visual encounter surveys ( = 0.249, SD = 0.702) was greater than that of pitfall sampling ( = 0.138, SD = 0.773) when cool temperatures and high humidity favor above-ground movement during the daytime. Light-touch ground searches of refuge habitats likely enhanced detection during visual surveys. Although the average detection rate was less, pitfall traps provided 24-hour sampling and were less affected by the experience of the surveyor and the occurrence of ground cover. In general, variation in seasonal behavior influenced detection regardless of method. The relatively higher cost and fixed nature of pitfall traps should be weighed against the ability to apply more cost-effective visual encounter surveys to a greater number of sites.

Temperature and Stress Dependence of Screw Dislocation Mobility in Nb-V-Ta Alloys Using Kinetic Monte Carlo Simulations

In this work we present simulations of thermally-activated screw dislocation motion in Nb-Ta-V alloys for two distinct scenarios, one where kink propagation is solely driven by chemical energy changes, i.e., thermodynamic energy differences, and another one where a migration barrier of 1.0 eV is added to such changes. The simulations have been performed using a kinetic Monte Carlo model for screw dislocation kinetics modified for complex lattice-level chemical environments. At low stresses, we find that dislocation motion in the case with no barrier is controlled by long waiting times due to slow nucleation rates and extremely fast kink propagation. Conversely, at high stress, the distribution of sampled time steps for both kink-pair nucleation and kink propagation events are comparable, resulting in continuous motion and faster velocities. In the case of the 1.0-eV kink propagation energy barrier, at low stresses kink motion becomes the rate-limiting step, leading to slow dynamics and large kink lateral pileups, while at high stresses both kink pair nucleation and kink propagation coexist on similar time scales. In the end, dislocation velocities differ by more than four orders of magnitude between both scenarios, emphasizing the need to have accurate calculations of kink energy barriers in the complex chemical environments inherent to these alloys.

Geospatial Analysis of Road Traffic Accidents and Emergency Response Optimization in Kano Metropolis, Nigeria

BackgroundIncrease in occurrence of road traffic accidents in Kano metropolis have resulted into continuous loss of lives, injuries and increased people’s exposure to risk. This study examined road traffic accidents emergency response within Kano metropolis with a view to enhancing its efficiency through establishing communication and synergy between Emergency Healthcare Facilities (EHCF), ambulances and accident hotspots. MethodsGPS surveying was conducted to obtain the location and attributes of the major EHCF, accident hotspots along the junctions of the highways and the 2 existing ambulances at Kano State Fire Service and Federal Road Safety Corp head offices (KSFS and FRSC). Road traffic data (vector format) was digitized from Worldview 3 satellite image (2018, 30cm spatial resolution) from which two major road classes were identified (highways and minor roads) along with their speed limits of 50km/hr and 30km/hr respectively. Time distances were determined based on length and speed limits. Nearest Neighbor and Network analysis (closest facility, shortest route and location-allocation) analyses were conducted. ResultsThe result revealed a variation in the distribution patterns of EHCH, ambulances and accident spots. Closest ambulance facility analysis shows that it takes the FRSC ambulance about 9.41 minutes to reach to accident spot 18 (Maiduguri Road, after NNPC), and 7.52 minutes to travel to AKTH as the closest EHCF. On the other hand, it takes the same ambulance about 3 times the time taken to spot 18 and 4 times the time taken to AKTH to reach to Court road incident spot (spot 16) and IRPH as the closest EHCF. This signifies greater chances of death of almost all victims across the metropolis due to inability to provide CPS within the first 4 minutes before reaching to the hospital. However, in case of Pediatric emergencies, the analysis of closest EHCF from accident spots revealed that it takes less than one minute to travel from accident spots 13, 14 and 15 to IRPH as the closest Pediatric EHCF. Equally, similar time is taken to travel from incident spots 20 and 23 to Sir Sunusi and MMS hospitals respectively. Location-allocation analysis identified eight new locations based on maximum of 4 minutes impedance cutoff from all directions towards the incidents spots. ConclusionIt is concluded that the prevailing road traffic accident emergency response system within the metropolis is inefficient. Therefore, more ambulances should be strategically positioned to fasten emergency response.

Are We Missing Something? The Skin Lesions Not Seen in Teledermatology

Background The suspected skin cancer electronic referral pathway was introduced in 2017. It requires general practitioners to add regional, close-up, and dermoscopic images to a lesion-specific referral template for a teledermatologist to review and advise on management. The virtual lesion clinic is a nurse-led clinic used since 2010 to obtain high-quality images for teledermoscopy assessment. A limitation of both services is the absence of a full-body examination. Objective This study aims to evaluate the number of skin cancers missed during teledermatology assessment. Methods This is a retrospective review of skin lesion referrals to dermatology. Suspected skin cancer referrals made in the latter half of 2020 were compared with referrals to the virtual lesion clinic during a similar time period in 2016. Results The study included 481 patients with 548 lesions in the 2020 suspected skin cancer cohort that were matched for age, sex, and ethnicity to 400 patients with 682 lesions in the 2016 virtual lesion clinic cohort. A total of 41 patients underwent subsequent specialist review in the suspected skin cancer cohort compared to 91 in the virtual lesion clinic cohort. A total of 20% of the suspected skin cancer cohort and 24% of the virtual lesion clinic cohort were found to have at least one additional lesion of concern. The majority of these were keratinocytic skin cancers; there were 2 and 0 additional melanomas or melanoma-in-situ, respectively. The virtual lesion clinic nurses identified additional lesions for imaging in 78 of 400 (20%) patients assessed in the virtual lesion clinic. The teledermatologist determined (author AO) that 73% of these additional lesions were malignant. Of the 548 lesions, 10 (2%) in the suspected skin cancer group were rereferred, none of which had a change in diagnosis. Out of 682 lesions, 16 (2%) in the virtual lesion clinic cohort were rereferred, 6 (1%) of which had a change in diagnosis. Conclusions Patients diagnosed with skin cancer often have multiple lesions of concern. Single-lesion teledermoscopy diagnoses have high concordance with in-person evaluation and histology; however, we have shown that in-person examination may reveal other suspicious lesions. The importance of a full-body skin examination should be emphasized to the referrer. Acknowledgments The Waikato Medical Research Foundation provided financial support for the study. Conflicts of Interest None declared.