Modern fluorescence-based concepts and methods to study biomolecular interactions

2017 ◽  
Vol 2 (2) ◽  
pp. 123-132 ◽  
Author(s):  
Maria Strianese ◽  
Maria Staiano ◽  
Alessandro Capo ◽  
Gabriella Pinto ◽  
Claudio Pellecchia ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Molecular Interactions ◽  
Biomolecular Interactions ◽  
Review Reports

This mini-review reports on the development of strategies which appear as potential tools to monitor molecular interactions. The goal is to highlight the advances in fluorescence spectroscopy, nanotechnology and probe chemistry to investigate molecular interactions.

Probing molecular interactions in single and live cells with fluorescence spectroscopy

2003 ◽  
Vol 22 (8) ◽  
pp. 537-543 ◽  
Author(s):  
Ujendra Kumar ◽  
Alessandra Baragli ◽  
Ramesh C. Patel
Keyword(s):  
Fluorescence Spectroscopy ◽  
Molecular Interactions ◽  
Live Cells

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

A preliminary study on the potential of front face fluorescence spectroscopy for the discrimination of Moroccan virgin olive oils and the prediction of their quality

2021 ◽  
Author(s):  
Hicham Zaroual ◽  
El Mestafa El Hadrami ◽  
Romdhane Karoui
Keyword(s):  
Fluorescence Spectroscopy ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Front Face ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Preliminary Study

This study examines the feasibility of using front face fluorescence spectroscopy (FFFS) to authenticate 41 virgin olive oil (VOO) samples collected from 5 regions in Morocco during 2 consecutive crop seasons.

Complexation of Synthetic Organic Dye Dapoxyl with cyclodextrins studied by fluorescence spectroscopy

2009 ◽  
Author(s):  
Wajih Al‐Soufi ◽  
Mercedes Novo ◽  
Jorge Bordello ◽  
Daniel Granadero
Keyword(s):  
Fluorescence Spectroscopy ◽  
Organic Dye
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