scholarly journals Access to site-specific Fc–cRGD peptide conjugates through streamlined expressed protein ligation

2016 ◽  
Vol 14 (40) ◽  
pp. 9549-9553 ◽  
Author(s):  
S. Frutos ◽  
J. B. Jordan ◽  
M. M. Bio ◽  
T. W. Muir ◽  
O. R. Thiel ◽  
...  
Keyword(s):  
Active Site ◽  
Bioactive Peptides ◽  
Expressed Protein Ligation ◽  
Peptide Conjugates ◽  
Site Specific ◽  
Protein Ligation ◽  
Split Intein ◽  
Efficient Route ◽  
Long Acting ◽  
Powerful Strategy

Conjugation of peptides to the Fc fragment of antibodies is a powerful strategy to generate long acting biotherapeutics. We show here an efficient route to obtain fully active, site-specific conjugates of synthetic bioactive peptides using a split intein based approach.

scholarly journals Analysis of Site‐Specific Phosphorylation of PTEN by Using Enzyme‐Catalyzed Expressed Protein Ligation

ChemBioChem ◽  
2019 ◽  
Vol 21 (1-2) ◽  
pp. 64-68 ◽  
Author(s):  
Samuel H. Henager ◽  
Stephanie Henriquez ◽  
Daniel R. Dempsey ◽  
Philip A. Cole
Keyword(s):  
Expressed Protein Ligation ◽  
Site Specific ◽  
Protein Ligation

scholarly journals Sortase-Tag Expressed Protein Ligation: Combining Protein Purification and Site-Specific Bioconjugation into a Single Step

2013 ◽  
Vol 85 (22) ◽  
pp. 11090-11097 ◽  
Author(s):  
Robert Warden-Rothman ◽  
Ilaria Caturegli ◽  
Vladimir Popik ◽  
Andrew Tsourkas
Keyword(s):  
Protein Purification ◽  
Single Step ◽  
Expressed Protein Ligation ◽  
Site Specific ◽  
Protein Ligation

Site-Specific Antibody Drug Conjugates Using Streamlined Expressed Protein Ligation

2018 ◽  
Vol 29 (11) ◽  
pp. 3503-3508 ◽  
Author(s):  
Silvia Frutos ◽  
Jose Luis Hernández ◽  
Anabel Otero ◽  
Carme Calvis ◽  
Jaume Adan ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Expressed Protein Ligation ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Protein Ligation ◽  
Drug Conjugates ◽  
Antibody Drug

scholarly journals Site-specific conjugation of RAFT polymers to proteins via expressed protein ligation

2013 ◽  
Vol 49 (25) ◽  
pp. 2566 ◽  
Author(s):  
Yan Xia ◽  
Shengchang Tang ◽  
Bradley D. Olsen
Keyword(s):  
Expressed Protein Ligation ◽  
Site Specific ◽  
Protein Ligation ◽  
Raft Polymers

Site-specific labeling of DNA–protein conjugates by means of expressed protein ligation

2007 ◽  
pp. 353-355 ◽  
Author(s):  
Marina Lovrinovic ◽  
Ljiljana Fruk ◽  
Hendrik Schröder ◽  
Christof M. Niemeyer
Keyword(s):  
Expressed Protein Ligation ◽  
Site Specific ◽  
Protein Ligation ◽  
Protein Conjugates

scholarly journals Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins

2012 ◽  
Vol 10 (2) ◽  
pp. 273-280 ◽  
Author(s):  
Lucia De Rosa ◽  
Aitziber L. Cortajarena ◽  
Alessandra Romanelli ◽  
Lynne Regan ◽  
Luca Domenico D'Andrea
Keyword(s):  
Specific Protein ◽  
Double Labeling ◽  
Expressed Protein Ligation ◽  
Site Specific ◽  
Protein Ligation ◽  
Repeat Proteins

Semisynthetic head-to-tail cyclized peptides obtained by combining protein trans-splicing and intramolecular expressed protein ligation

2021 ◽  
Author(s):  
Shubhendu Palei ◽  
Henning D. Mootz
Keyword(s):  
Expressed Protein Ligation ◽  
Peptide Bonds ◽  
Protein Ligation ◽  
Native Peptide ◽  
Split Intein ◽  
Trans Splicing ◽  
Macrocyclic Peptides

A dual-intein approach for the preparation of head-to-tail macrocyclic peptides is reported, where synthetic and genetically encoded fragments are ligated by two native peptide bonds. A split intein ligates the...

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