Sensitive and label-free discrimination of 5-hydroxymethylcytosine and 5-methylcytosine in DNA by ligation-mediated rolling circle amplification

2018 ◽  
Vol 54 (62) ◽  
pp. 8602-8605 ◽  
Author(s):  
Zi-yue Wang ◽  
Meng Wang ◽  
Yan Zhang ◽  
Chun-yang Zhang

We develop a label-free fluorescence method for sensitive discrimination of 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) by ligation-mediated rolling circle amplification.

2020 ◽  
Vol 56 (39) ◽  
pp. 5243-5246
Author(s):  
Meng Liu ◽  
Di Zhang ◽  
Xuechong Zhang ◽  
Qinfeng Xu ◽  
Fei Ma ◽  
...  

We develop a label-free fluorescence method for ultrasensitive detection of apoptosis-associated caspase activity based on branched rolling circle amplification.


RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.


The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2884-2889 ◽  
Author(s):  
Xingti Liu ◽  
Qingwang Xue ◽  
Yongshun Ding ◽  
Jing Zhu ◽  
Lei Wang ◽  
...  

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.


The Analyst ◽  
2020 ◽  
Vol 145 (24) ◽  
pp. 8002-8007
Author(s):  
Hyobeen Lee ◽  
Dong-Min Kim ◽  
Dong-Eun Kim

Label-free fluorometric detection of influenza viral RNA by strand displacement coupled with rolling circle amplification.


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