Site directed mutagenesis of insulin-degrading enzyme allows singling out the molecular basis of peptidase versus E1-like activity: the role of metal ions

Metallomics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 278-281 ◽  
Author(s):  
Francesco Bellia ◽  
Valeria Lanza ◽  
Ikhlas Mohamed Mohamud Ahmed ◽  
Sara Garcia-Vinuales ◽  
Eva Veiss ◽  
...  
Keyword(s):  
Metal Ions ◽  
Molecular Basis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Insulin Degrading Enzyme ◽  
Degrading Enzyme

Four specifically designed IDE mutants have been used to unveil the molecular basis of peptidase versus E1-like activity of the enzyme.

scholarly journals Probing the role of metal ions in the mechanism of inositol monophosphatase by site-directed mutagenesis

1993 ◽  
Vol 217 (1) ◽  
pp. 281-287 ◽  
Author(s):  
Scott J. POLLACK ◽  
Michael R. KNOWLES ◽  
John R. ATACK ◽  
Howard B. BROUGHTON ◽  
C. Ian RAGAN ◽  
...  
Keyword(s):  
Metal Ions ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Inositol Monophosphatase

scholarly journals Site-directed mutagenesis of tyrosine hydroxylase. Role of serine 40 in catalysis.

1992 ◽  
Vol 267 (36) ◽  
pp. 25754-25758
Author(s):  
J Wu ◽  
D Filer ◽  
A.J. Friedhoff ◽  
M Goldstein
Keyword(s):  
Tyrosine Hydroxylase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals The role of Val68(E11) in ligand binding to sperm whale myoglobin. Site-directed mutagenesis of a synthetic gene.

1990 ◽  
Vol 265 (20) ◽  
pp. 11788-11795
Author(s):  
K D Egeberg ◽  
B A Springer ◽  
S G Sligar ◽  
T E Carver ◽  
R J Rohlfs ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Sperm Whale ◽  
Site Directed Mutagenesis ◽  
Synthetic Gene ◽  
Directed Mutagenesis ◽  
Sperm Whale Myoglobin

Probing the Role of Tyr 64 ofTreponema denticolaCystalysin by Site-Directed Mutagenesis and Kinetic Studies†

Biochemistry ◽  
2005 ◽  
Vol 44 (42) ◽  
pp. 13970-13980 ◽  
Author(s):  
Barbara Cellini ◽  
Mariarita Bertoldi ◽  
Riccardo Montioli ◽  
Carla Borri Voltattorni
Keyword(s):  
Kinetic Studies ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis
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