scholarly journals Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy

RSC Advances ◽  
2019 ◽  
Vol 9 (21) ◽  
pp. 11960-11967 ◽  
Author(s):  
Zhongzhi Liu ◽  
Dan Luo ◽  
Fangling Ren ◽  
Fengying Ran ◽  
Wei Chen ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Signal Amplification ◽  
Rnase H ◽  
Ribonuclease H ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amplification Strategy ◽  
Fluorescent Aptasensor

An aptamer-based method for the ultrasensitive fluorescence detection of C-reactive protein (CRP) was developed using the ribonuclease H (RNase H) assisted DNA recycling signal amplification strategy.

A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16149-16153 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyowon Jang ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Signal Amplification ◽  
Rnase H ◽  
Label Free ◽  
Activity Assay ◽  
G Quadruplex ◽  
Catalytic Hairpin Assembly ◽  
Amplification Strategy

A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.

An enzyme-initiated DNAzyme motor for RNase H activity imaging in living cell

2020 ◽  
Vol 56 (4) ◽  
pp. 639-642 ◽  
Author(s):  
Namin Hu ◽  
Yafang Wang ◽  
Chang Liu ◽  
Manman He ◽  
Cunpeng Nie ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Living Cell ◽  
Rnase H ◽  
Amplification Strategy

A signal amplification strategy based on an enzyme-initiated DNAzyme motor for sensitive imaging of RNase H activity in living cell.

Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6689-6697 ◽  
Author(s):  
Han Zhao ◽  
Ying Yan ◽  
Mingjian Chen ◽  
Tingting Hu ◽  
Kefeng Wu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Method ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A facile fluorescent method has been developed for polynucleotide kinase detection based on copper nanoparticles and exonuclease III-assisted signal amplification.

A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection

2020 ◽  
Vol 56 (48) ◽  
pp. 6579-6582 ◽  
Author(s):  
Shengyu Chen ◽  
Jingjin Zhao ◽  
Xing Yang ◽  
Shulin Zhao ◽  
Yi-Ming Liu
Keyword(s):  
Fluorescence Detection ◽  
Signal Amplification ◽  
Single Cells ◽  
Microchip Electrophoresis ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection ◽  
Intracellular Signal ◽  
Amplification Strategy

An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis laser-induced fluorescence detection.

An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 1673-1679 ◽  
Author(s):  
Yafang Wang ◽  
Namin Hu ◽  
Chang Liu ◽  
Cunpeng Nie ◽  
Manman He ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rnase H ◽  
Sensitive Detection ◽  
Activity Detection ◽  
Walking Machine ◽  
Amplification Strategy

A signal amplification strategy based on an RNase H-powered DNA walking machine for RNase H activity detection.

Redox active molybdophosphate produced by Cu3(PO4)2nanospheres for enhancing enzyme-free electrochemical immunoassay of C-reactive protein

2017 ◽  
Vol 41 (20) ◽  
pp. 11867-11871 ◽  
Author(s):  
Xiaofeng Tan ◽  
Lianhua Zhang ◽  
Xiaobo Deng ◽  
Luyang Miao ◽  
He Li ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Electrochemical Immunoassay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Redox Active

Redox-active molybdophosphate produced by Cu3(PO4)2nanospheres has been directly employed for signal amplification of an enzyme-free electrochemical immunosensor.

A dual signal amplification strategy for the highly sensitive fluorescence detection of nucleic acids

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1219-1226 ◽  
Author(s):  
Jingjing Zhang ◽  
Chunyuan Song ◽  
Huiling Zhou ◽  
Juan Jia ◽  
Yinna Dai ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Detection ◽  
Signal Amplification ◽  
Fluorescence Assay ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A dual signal amplification strategy comprising target-triggered recycling and DSN-mediated amplifications was designed and proposed for a highly sensitive fluorescence assay of nucleic acids.

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