A dual signal amplification strategy for the highly sensitive fluorescence detection of nucleic acids

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1219-1226 ◽  
Author(s):  
Jingjing Zhang ◽  
Chunyuan Song ◽  
Huiling Zhou ◽  
Juan Jia ◽  
Yinna Dai ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Detection ◽  
Signal Amplification ◽  
Fluorescence Assay ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A dual signal amplification strategy comprising target-triggered recycling and DSN-mediated amplifications was designed and proposed for a highly sensitive fluorescence assay of nucleic acids.

Highly sensitive electrochemiluminescence aptasensor based on dual-signal amplification strategy for kanamycin detection

2020 ◽  
Vol 737 ◽  
pp. 139785
Author(s):  
Shuting Cheng ◽  
Hui Zhang ◽  
Jingcheng Huang ◽  
Rui Xu ◽  
Xia Sun ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification

Hairpin DNA-fueled dynamic self-assembly of three-arm DNA branched junctions consisting of active DNAzyme structures for enzyme-free ultrasensitive detection of nucleic acids

2016 ◽  
Vol 8 (47) ◽  
pp. 8262-8265 ◽  
Author(s):  
Ke Yang ◽  
Ming Zeng ◽  
Xing He ◽  
Jianming Li ◽  
Dinggeng He
Keyword(s):  
Nucleic Acids ◽  
Self Assembly ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Hairpin Dna ◽  
Amplification Strategy ◽  
Dual Signal Amplification

An enzyme-free dual signal amplification strategy based on programmable molecular hairpins has been developed for amplified detection of DNA via the hairpin DNA-fueled dynamic self-assembly of three-arm DNAzyme.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

A highly sensitive photoelectrochemical VEGF165 biosensor with a dual signal amplification strategy by using AgVO3 as a photoactive material

2019 ◽  
Vol 55 (56) ◽  
pp. 8076-8078 ◽  
Author(s):  
Huimei Da ◽  
Yaling Liu ◽  
Mengjie Li ◽  
Ruo Yuan ◽  
Hongyan Liu ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Vascular Endothelial ◽  
Background Signal ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification ◽  
Endothelial Growth Factor

We developed a novel “signal-on” photoelectrochemical (PEC) aptasensor with a near-zero background signal by using AgVO3 as a single photoactive material for the sensitive detection of vascular endothelial growth factor (VEGF165).

Highly sensitive electrochemical detection of circulating EpCAM-positive tumor cells using a dual signal amplification strategy

2021 ◽  
pp. 130087
Author(s):  
Ayemeh Bagheri Hashkavayi ◽  
Byung Seok Cha ◽  
Sung Hyun Hwang ◽  
Jimin Kim ◽  
Ki Soo Park
Keyword(s):  
Tumor Cells ◽  
Electrochemical Detection ◽  
Signal Amplification ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification

Ultrasensitive sandwich RNA-aptasensor based on dual-signal amplification strategy for highly sensitive neomycin detection

Food Control ◽  
2021 ◽  
pp. 108445
Author(s):  
Falan Li ◽  
Xiaolin Gao ◽  
Xiaoyang Wang ◽  
Yemin Guo ◽  
Xia Sun ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

The Analyst ◽  
2019 ◽  
Vol 144 (5) ◽  
pp. 1671-1678 ◽  
Author(s):  
Xiaoqiang Liu ◽  
Yunfei Tang ◽  
Peipei Liu ◽  
Liwei Yang ◽  
Lele Li ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Signal Amplification ◽  
Electrochemical Aptasensor ◽  
Ternary Composite ◽  
Highly Sensitive ◽  
Amplification System ◽  
Amplification Strategy ◽  
Dual Signal Amplification ◽  
Signal Amplification System

In this work, a sensitive and selective electrochemical aptasensor for detection of microcystin-LR was developed based on a dual signal amplification system consisted of a novel ternary composite and horseradish peroxidase.

A carboxylated graphene nanodisks/glucose oxidase nanotags and Mn:CdS/TiO2 matrix based dual signal amplification strategy for ultrasensitive photoelectrochemical detection of tumor markers

The Analyst ◽  
2017 ◽  
Vol 142 (24) ◽  
pp. 4647-4654 ◽  
Author(s):  
Jie Song ◽  
Shuo Wu ◽  
Xinlan Yang ◽  
Jingli Yuan
Keyword(s):  
Glucose Oxidase ◽  
Tumor Markers ◽  
Signal Amplification ◽  
Highly Sensitive ◽  
Graphene Nanodisks ◽  
Tio2 Matrix ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A graphene nanodisk and glucose oxidase based dual signal amplification strategy was developed for a highly sensitive PEC immunoassay of CEA.

scholarly journals Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification

ACS Omega ◽  
2020 ◽  
Vol 5 (50) ◽  
pp. 32738-32743
Author(s):  
Fubing Xiao ◽  
Jie Liu ◽  
Qinghui Guo ◽  
Zhibo Du ◽  
Hong Li ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Amplification Strategy ◽  
Dual Signal Amplification

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Sign in / Sign up

Export Citation Format

Share Document