An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 1673-1679 ◽  
Author(s):  
Yafang Wang ◽  
Namin Hu ◽  
Chang Liu ◽  
Cunpeng Nie ◽  
Manman He ◽  
...  

A signal amplification strategy based on an RNase H-powered DNA walking machine for RNase H activity detection.

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16149-16153 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyowon Jang ◽  
Ki Soo Park ◽  
Hyun Gyu Park

A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.


2020 ◽  
Vol 56 (4) ◽  
pp. 639-642 ◽  
Author(s):  
Namin Hu ◽  
Yafang Wang ◽  
Chang Liu ◽  
Manman He ◽  
Cunpeng Nie ◽  
...  

A signal amplification strategy based on an enzyme-initiated DNAzyme motor for sensitive imaging of RNase H activity in living cell.


2018 ◽  
Vol 42 (17) ◽  
pp. 14642-14647 ◽  
Author(s):  
Yancui Jiao ◽  
Jiayun Fu ◽  
Wenjie Hou ◽  
Zhaoqiang Shi ◽  
Yemin Guo ◽  
...  

A homogeneous type of electrochemical aptasensor was designed based upon the principle of target-induced and tool enzyme-assisted signal amplification, which was employed for the detection of profenofos residues.


2019 ◽  
Vol 7 (29) ◽  
pp. 4520-4527 ◽  
Author(s):  
Han Zhao ◽  
Changbei Ma ◽  
Ying Yan ◽  
Mingjian Chen

A sensitive fluorometric method for DNA methyltransferase activity detection based on graphene oxide and RNase H-assisted signal amplification.


2020 ◽  
Vol 56 (87) ◽  
pp. 13445-13448
Author(s):  
Weimin Tian ◽  
Xiaoling Liu ◽  
Gaoting Wang ◽  
Chenghui Liu

A hyperbranched RCA-assisted transcription and CRISPR-Cas12a-based triplex signal amplification strategy is developed for the sensitive detection of microRNA.


2014 ◽  
Vol 6 (11) ◽  
pp. 3598-3603 ◽  
Author(s):  
Shuang Guo ◽  
Fan Yang ◽  
Yulin Zhang ◽  
Yong Ning ◽  
Qunfeng Yao ◽  
...  

A graphene oxide-based fluorescent sensing system for sensitive detection of miRNA with a duplex-specific nuclease-aided signal amplification strategy.


2017 ◽  
Vol 53 (2) ◽  
pp. 455-458 ◽  
Author(s):  
Yingfeng Qin ◽  
Liangliang Zhang ◽  
Shuting Li ◽  
Jingjin Zhao ◽  
Yong Huang ◽  
...  

A microchip electrophoresis-based fluorescence signal amplification strategy was developed for highly sensitive detection of biomolecules.


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