scholarly journals A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

RSC Advances ◽  
2020 ◽  
Vol 10 (40) ◽  
pp. 23931-23935
Author(s):  
Codruţa C. Popescu ◽  
Marius C. Stoian ◽  
Lia-Maria Cucos ◽  
Anca G. Coman ◽  
Antonio Radoi ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Metal Ions ◽  
Protein Purification ◽  
Metal Ion ◽  
Solid Support ◽  
Chelating Ligand ◽  
Metal Ion Affinity Chromatography

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC).

Evaluation of Immobilized Metal-Ion Affinity Chromatography (IMAC) as a Technique for IgG1 Monoclonal Antibodies Purification: The Effect of Chelating Ligand and Support

2009 ◽  
Vol 160 (7) ◽  
pp. 2148-2165 ◽  
Author(s):  
I. T. L. Bresolin ◽  
M. Borsoi-Ribeiro ◽  
W. M. S. C. Tamashiro ◽  
E. F. P. Augusto ◽  
M. A. Vijayalakshmi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Affinity Chromatography ◽  
Metal Ion ◽  
Chelating Ligand ◽  
Metal Ion Affinity Chromatography

Adsorption of endotoxins on Ca2+ iminodiacetic acid by metal ion affinity chromatography

2013 ◽  
Vol 30 (11) ◽  
pp. 1194-1202
Author(s):  
LOPES André Moreni ◽  
ROMEU Jorge Sánchez ◽  
MEIRELES Rolando Páez ◽  
PERERA Gabriel Marquez ◽  
MORALES Rolando Perdomo ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Metal Ion ◽  
Iminodiacetic Acid ◽  
Metal Ion Affinity Chromatography

Sequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography

2013 ◽  
Vol 86 (1) ◽  
pp. 685-693 ◽  
Author(s):  
Chia-Feng Tsai ◽  
Chuan-Chih Hsu ◽  
Jo-Nan Hung ◽  
Yi-Ting Wang ◽  
Wai-Kok Choong ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Metal Ion ◽  
Metal Ion Affinity Chromatography
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