scholarly journals Correction: Estimating the cooperativity of PROTAC-induced ternary complexes using 19F NMR displacement assay

2022 ◽  
Author(s):  
Guilherme Vieira de Castro ◽  
Alessio Ciulli
Keyword(s):  
Ternary Complexes ◽  
19F Nmr ◽  
Displacement Assay

Correction for ‘Estimating the cooperativity of PROTAC-induced ternary complexes using 19F NMR displacement assay’ by Guilherme Vieira de Castro et al., RSC Med. Chem., 2021, 12, 1765–1770, DOI: 10.1039/D1MD00215E.

scholarly journals Estimating the cooperativity of PROTAC-induced ternary complexes using 19F NMR displacement assay

2021 ◽  
Author(s):  
Guilherme Castro ◽  
Alessio Ciulli
Keyword(s):  
Ternary Complexes ◽  
19F Nmr ◽  
Fluorine Nmr ◽  
Competition Binding ◽  
Displacement Assay

Cooperativity is an important parameter to understand the ternary complexes formed by protein degraders. We developed fluorine NMR competition binding experiments to determine cooperativity of PROTACs. We show applicability to...

scholarly journals 19F NMR indicator displacement assay using a synthetic receptor with appended paramagnetic relaxation agent

2014 ◽  
Vol 50 (72) ◽  
pp. 10499-10501 ◽  
Author(s):  
Adam J. Plaunt ◽  
Kasey J. Clear ◽  
Bradley D. Smith
Keyword(s):  
Signal Intensity ◽  
Paramagnetic Relaxation ◽  
19F Nmr ◽  
Synthetic Receptor ◽  
Displacement Assay

A 19F NMR indicator displacement assay detects phosphorylated analytes with amplified signal intensity.

Use of 19F NMR for studies of the complexation of gallium (III) by 5-fluoro-8-hydroxyquinoline

1998 ◽  
Vol 95 (2) ◽  
pp. 395-400
Author(s):  
O. Jarjayes ◽  
S. Hamman ◽  
C. G. Beguin
Keyword(s):  
19F Nmr

19F-NMR in Target-based Drug Discovery

2019 ◽  
Vol 26 (26) ◽  
pp. 4964-4983 ◽  
Author(s):  
CongBao Kang
Keyword(s):  
Drug Discovery ◽  
Conformational Changes ◽  
Protein Structures ◽  
19F Nmr ◽  
Binding Affinities ◽  
Protein Ligand Interactions ◽  
Compound Libraries ◽  
Ligand Interactions ◽  
Reference Ligand ◽  
Hit Identification

Solution NMR spectroscopy plays important roles in understanding protein structures, dynamics and protein-protein/ligand interactions. In a target-based drug discovery project, NMR can serve an important function in hit identification and lead optimization. Fluorine is a valuable probe for evaluating protein conformational changes and protein-ligand interactions. Accumulated studies demonstrate that 19F-NMR can play important roles in fragment- based drug discovery (FBDD) and probing protein-ligand interactions. This review summarizes the application of 19F-NMR in understanding protein-ligand interactions and drug discovery. Several examples are included to show the roles of 19F-NMR in confirming identified hits/leads in the drug discovery process. In addition to identifying hits from fluorinecontaining compound libraries, 19F-NMR will play an important role in drug discovery by providing a fast and robust way in novel hit identification. This technique can be used for ranking compounds with different binding affinities and is particularly useful for screening competitive compounds when a reference ligand is available.

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