A single dual-targeting fluorescent probe enables to explore the correlation between plasma membrane and lysosomes

2022 ◽  
Author(s):  
Shimo Yu ◽  
Shining Wu ◽  
Jing Zhang ◽  
Xinfu Zhao ◽  
Xiaochan Liu ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Fluorescent Probe ◽  
Waste Disposal ◽  
Normal Cell ◽  
Cell Activity ◽  
Dual Targeting

The interactions between organelles can maintain normal cell activity. Lysosomes, as waste disposal systems of cells, have many important interactions with plasma membrane, especially in the repairing of cracked plasma...

scholarly journals Dual targeting of a virus movement protein to ER and plasma membrane subdomains is essential for plasmodesmata localization

2017 ◽  
Vol 13 (6) ◽  
pp. e1006463 ◽  
Author(s):  
Kazuya Ishikawa ◽  
Masayoshi Hashimoto ◽  
Akira Yusa ◽  
Hiroaki Koinuma ◽  
Yugo Kitazawa ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Movement Protein ◽  
Virus Movement ◽  
Dual Targeting

scholarly journals Caspase-3 activation decreases lipid order in the outer plasma membrane leaflet during apoptosis: A fluorescent probe study

2017 ◽  
Vol 1859 (10) ◽  
pp. 2123-2132 ◽  
Author(s):  
Kyrylo A. Pyrshev ◽  
Semen O. Yesylevskyy ◽  
Yves Mély ◽  
Alexander P. Demchenko ◽  
Andrey S. Klymchenko
Keyword(s):  
Plasma Membrane ◽  
Fluorescent Probe ◽  
Caspase 3 ◽  
Lipid Order ◽  
Outer Plasma Membrane

Internalization of the lipophilic fluorescent probe trimethylamino-diphenylhexatriene follows the endocytosis and recycling of the plasma membrane in cells

1990 ◽  
Vol 1030 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Dominique Illinger ◽  
Philippe Poindron ◽  
Paul Fonteneau ◽  
Manuel Modollel ◽  
Jean-Georges Kuhry
Keyword(s):  
Plasma Membrane ◽  
Fluorescent Probe ◽  
In Cells

In Vitro DNA/BSA Binding, Anticancer and Normal Cell Activity of Pd(II) Complexes: Substitution Behaviour and Computational Study

2018 ◽  
Vol 3 (13) ◽  
pp. 3871-3885 ◽  
Author(s):  
Subhajit Mukherjee ◽  
Ishani Mitra ◽  
Venkata P. Reddy B. ◽  
Payel Das ◽  
Bashkim Misini ◽  
...  
Keyword(s):  
Normal Cell ◽  
Computational Study ◽  
Cell Activity ◽  
Bsa Binding

scholarly journals Pericellular Hyaluronan Coat Visualized in Live Cells With a Fluorescent Probe Is Scaffolded by Plasma Membrane Protrusions

2008 ◽  
Vol 56 (10) ◽  
pp. 901-910 ◽  
Author(s):  
Kirsi Rilla ◽  
Riikka Tiihonen ◽  
Anne Kultti ◽  
Markku Tammi ◽  
Raija Tammi
Keyword(s):  
Plasma Membrane ◽  
Fluorescent Probe ◽  
Fluorescent Protein ◽  
Cell Types ◽  
Live Cells ◽  
Random Coils ◽  
Inherent Feature ◽  
Contrast Microscopy ◽  
Membrane Protrusions ◽  
Green Fluorescent

Many cell types wear up to 20-μm-wide hyaluronidase-sensitive surface coats, detected by exclusion of sedimenting particles like fixed erythrocytes. The structure of the coat is enigmatic, being apparently too thick to be accounted by random coils or even extended chains of just hyaluronan attached to cell surface. We have shown that hyaluronan synthesis enforced by green fluorescent protein-hyaluronan synthase transfection creates microvillous protrusions. The idea that the plasma membrane protrusions rather than hyaluronan alone is responsible for the exclusion space was studied with a fluorescent probe for hyaluronan and a dye with membrane affinity, applied to live cell cultures. Mesothelial and smooth muscle cells, fibroblasts, and chondrocytes, all known for their endogenously active hyaluronan synthesis, showed hyaluronan-coated plasma membrane protrusions, barely visible in phase contrast microscopy. Treatment with hyaluronidase and inhibition of hyaluronan synthesis caused retraction of the protrusions unless they were attached to substratum. Hyaluronan and the exclusion space were reduced, but did not disappear, by purified hyaluronan hexasaccharides that compete with hyaluronan attached to CD44. The results suggest that slender plasma membrane protrusions are an inherent feature of hyaluronan coats, form their scaffold, and largely result from ongoing hyaluronan synthesis in their plasma membrane. This manuscript contains online supplemental material at www.jhc.org . Please visit this article online to view these materials.

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