Peptide synthesis. Part 8. A system for solid-phase synthesis under low pressure continuous flow conditions

1986 ◽  
pp. 125 ◽  
Author(s):  
Alison Dryland ◽  
Robert C. Sheppard
Keyword(s):  
Peptide Synthesis ◽  
Continuous Flow ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Low Pressure ◽  
Flow Conditions ◽  
Phase Synthesis

Colour-Monitored Solid-Phase Multiple Peptide Synthesis under Low-Pressure Continuous-Flow Conditions. Synthesis of Medium-Size Peptides: The Propart of Human Procathepsin D and the Growth-Hormone Releasing Factor

1993 ◽  
Vol 58 (2) ◽  
pp. 435-444 ◽  
Author(s):  
Josef Vágner ◽  
Viktor Krchňák ◽  
Josef Pícha ◽  
Drahomíra Píchová ◽  
Martin Fusek
Keyword(s):  
Growth Hormone ◽  
Peptide Synthesis ◽  
Continuous Flow ◽  
Solid Phase ◽  
Low Pressure ◽  
Reversed Phase ◽  
Bromophenol Blue ◽  
Medium Size ◽  
Flow Conditions ◽  
Growth Hormone Releasing Factor

The synthesis of two medium-size peptide amides, the propart of human procathepsin D (1-45) and the bovine growth-hormon releasing factor (1-44) has been accomplished by the solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions on standard methylbenzhydrylamine polystyrene-based resin in concatenated flow reactors with adjustable volume. The completeness of acylation of resin-bound free amino groups was monitored by the acid-base indicator bromophenol blue. The efficiency of the coupling reaction was increased by exposure to elevated temperature in an ultrasonic bath. Crude peptides were purified on a mass-overloaded Vydac C18 reversed-phase semipreparative HPLC column. The synthetic propart of human procathepsin D (1-45) exhibited inhibition activity to bovine cathepsin D and four other aspartic proteases. Significantly increased plasma concentration in gilts was determined after application of synthetic bovine growth-hormone releasing factor (1-44).

scholarly journals The Breaking Beads Approach for Photocleavage from Solid Support

2020 ◽  
Author(s):  
yasmeen bakhatan ◽  
Israel Alshanski ◽  
Dana Grunhaus ◽  
Mattan Hurevich
Keyword(s):  
Continuous Flow ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
High Energy ◽  
Solid Support ◽  
Phase Synthesis ◽  
Polystyrene Beads ◽  
Magnetic Stirring ◽  
Photolabile Linkers

Photocleavage from polystyrene beads is a pivotal reaction for solid phase synthesis that relies on photolabile linkers. The photocleavage, usually performed by batch irradiation, suffers from incomplete and slow cleavage. To overcome these issues, continuous flow and high-energy lamps are frequently used, but these setups are hazardous, technically challenging, and expensive. We developed a photocleavage approach that relies on a benchtop LED lamp and magnetic stirring. In this approach, we crush the beads instead of keeping their integrity to increase the yield of photocleavage. This approach proved very efficient for photocleavage of protected oligosaccharides.

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