The biosynthesis of kojic acid. 2. The occurrence of aldolase and triosephosphate isomerase in Aspergillus species and their relationship to kojic acid biosynthesis

Efforts have been made to develop a simple chemically defined resuspended mycelial system which may be used for carrying out fundamental studies regarding the mechanism of kojic acid biosynthesis. As a first step, it was found that mycelia grown in yeast extract sucrose (YES) medium and resuspended in YES medium or in 0.2 M phosphate buffer, pH 6.5, supplemented with 20% glucose or sucrose produced kojic acid almost to the same extent as in the case of growth medium. No kojic acid was formed if buffers or media used for resuspension lacked carbohydrate. Intact mycelia preincubated in buffer alone for 7 days and 3-week-old mycelia could still form kojic acid in large amounts if resuspended in buffer containing glucose. The amount of kojic acid produced by the intact mycelia was found to be more than that produced by the disrupted mycelia. In contrast with static resuspension studies, when Aspergillus flavus mycelia were resuspended in flasks placed on a rotary shaker, much smaller amounts of kojic acid were synthesized.

Download Full-text

Enzymes Relevant to Kojic Acid Biosynthesis in Aspergillus flavus

Microbiology ◽

10.1099/00221287-127-1-131 ◽

1981 ◽

Vol 127 (1) ◽

pp. 131-136

Author(s):

P. BAJPAI ◽

P.K. AGRAWALA ◽

L. VISHWANATHAN

Keyword(s):

Aspergillus Flavus ◽

Kojic Acid ◽

Acid Biosynthesis

Download Full-text

Identification and characterization of genes involved in kojic acid biosynthesis in Aspergillus flavus

Annals of Microbiology ◽

10.1007/s13213-017-1297-8 ◽

2017 ◽

Vol 67 (10) ◽

pp. 691-702 ◽

Cited By ~ 7

Author(s):

Hala A. M. Ammar ◽

Ali Y. Srour ◽

Saeid M. Ezzat ◽

Asmaa M. Hoseny

Keyword(s):

Aspergillus Flavus ◽

Kojic Acid ◽

Acid Biosynthesis ◽

Identification And Characterization

Download Full-text

Analysis of Kojic Acid Biosynthesis in Aspergillus oryzae

JOURNAL OF THE BREWING SOCIETY OF JAPAN ◽

10.6013/jbrewsocjapan.112.9 ◽

2017 ◽

Vol 112 (1) ◽

pp. 9-14

Author(s):

Motoaki SANO

Keyword(s):

Aspergillus Oryzae ◽

Kojic Acid ◽

Acid Biosynthesis

Download Full-text

Electronic study of fresh enzyme complexes of antifungal drugs-P450 and Aspergillus kojic acid biosynthesis. W: w saccharose flavus: fructose as a substratum

10.36295/asro.2019.22048 ◽

2019 ◽

Vol 22 (04) ◽

pp. 65-72

Author(s):

Khansaa Basem Fadhil ◽

Moazaz Abd Alrida Majeed ◽

Mohammed Ahmed Mustafa

Keyword(s):

Kojic Acid ◽

Antifungal Drugs ◽

Acid Biosynthesis ◽

Enzyme Complexes

Download Full-text

The role of copper(I)-nicotinic acid complex on kojic acid biosynthesis byAspergillus flavus

Journal of Basic Microbiology ◽

10.1002/jobm.3620270105 ◽

1987 ◽

Vol 27 (1) ◽

pp. 29-33 ◽

Cited By ~ 9

Author(s):

S. E. Megalla ◽

A. Y. Nassar ◽

M. A. S. Gohar

Keyword(s):

Nicotinic Acid ◽

Kojic Acid ◽

Acid Biosynthesis ◽

Acid Complex

Download Full-text

Localization of oleic acid biosynthesis enzymes in the proplastids of developing castor endosperm

Canadian Journal of Botany ◽

10.1139/b72-043 ◽

1972 ◽

Vol 50 (2) ◽

pp. 323-326 ◽

Cited By ~ 32

Author(s):

B. F. Zilkey ◽

D. T. Canvin

Keyword(s):

Oleic Acid ◽

Fatty Acid ◽

Electron Micrographs ◽

Castor Bean ◽

Triosephosphate Isomerase ◽

Acid Synthesis ◽

Major Constituent ◽

Acid Biosynthesis ◽

Peak Density ◽

Malonyl Coa

The particulate fatty acid synthesizing system from the developing castor bean endosperm was separated using sucrose density gradients. The particulate fatty acid synthesizing enzymes were associated with a particle of peak density 1.21 g∙(cm3)−1. Based on the presence of triosephosphate isomerase and the appearance of the particles in electron micrographs the major constituent of this fraction was identified as proplastids. It appears that in this tissue the proplastid contains the complete enzyme complement for oleic acid synthesis from acetyl or malonyl-CoA.

Download Full-text