Cytosolic free Ca2+ in single rat heart cells during anoxia and reoxygenation
Keyword(s):
Free Ca2+ in the cytosol ([Ca2+]i) of individual rat ventricle cells injected with aequorin was measured under anoxia. In glucose-free medium myocytes spontaneously shortened after about 60 min, although [Ca2+]i was still at or near resting levels. However, within minutes a net inward movement of Ca2+ across the sarcolemma developed and [Ca2+]i began to rise. Provided oxygen was readmitted before [Ca2+]i exceeded 2-3 microM, cells were able to restore [Ca2+]i to resting levels through caffeine-sensitive sequestration of Ca2+ in the sarcoplasmic reticulum. We suggest that Ca2+-independent shortening of anoxic cardiomyocytes reflects onset of rigor which triggers loss of [Ca2+]i homoeostasis.
1993 ◽
Vol 119
(1-2)
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pp. 51-57
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1981 ◽
Vol 256
(6)
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pp. 2856-2862
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1992 ◽
Vol 267
(14)
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pp. 9917-9924
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Keyword(s):
1973 ◽
Vol 57
(1)
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pp. 109-116
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