Trisomy 3 in marginal zone B-cell lymphoma: a study based on cytogenetic analysis and fluorescence in situ hybridization

Abstract The translocation of chromosome 11, long arm, region 2, band 1, to chromosome 18, long arm, region 2, band 1 (t(11;18)(q21;q21)) represents a recurrent chromosomal abnormality in extranodal marginal zone B-cell lymphoma (MZBCL) of mucosa-associated lymphoid tissue (MALT) type and leads to a fusion of the apoptosis inhibitor-2 (API2) gene on chromosome 11 and the MALT lymphoma-associated translocation (MLT) gene on chromosome 18. A 2-color fluorescence in situ hybridization (FISH) assay, which can be used for the detection of t(11;18) in interphase nuclei and metaphase chromosomes on fresh and archival tumor tissue, was developed. The P1 artificial chromosome (PAC) clone located immediately telomeric to the MLT gene and the PAC clone spanning the API2 gene were differentially labeled and used to visualize the derivative chromosome 11 resulting from t(11;18), as evident by the overlapping or juxtaposed red and green fluorescent signals. The assay was applied to interphase nuclei of 20 cases with nonmalignant conditions and 122 B-cell non-Hodgkin's lymphomas (NHLs). The latter group comprised 20 cases of nodal follicle center cell lymphoma and diffuse large B-cell NHL, 10 cases of gastric diffuse large B-cell lymphoma, 10 cases of hairy cell leukemia, and 82 cases of MZBCL (41 extranodal from various locations, 19 nodal, and 22 splenic MZBCL) including 35 cases with an abnormal karyotype, 2 of which revealed t(11;18). By interphase FISH, t(11;18) was detected in 8 gastrointestinal low-grade MALT-type lymphomas including the 2 cytogenetically t(11;18)+ cases. In the 8 t(11;18)+ cases, the FISH results were confirmed by reverse transcriptase–polymerase chain reaction (RT-PCR) usingAPI2 and MLT specific primers. Our results indicate that t(11;18)(q21;q21) specifically characterizes a subgroup of low-grade MZBCL of the MALT-type and that the FISH assay described here is a highly specific and rapid test for the detection of this translocation.

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Spectrum of Signet Ring Cell Morphology in B-Cell Lymphoma: Clinicopathologic, Immunohistochemical and Fluorescence In Situ Hybridization Studies of Six Cases

American Journal of Clinical Pathology ◽

10.1093/ajcp/144.suppl2.165 ◽

2015 ◽

Vol 144 (suppl 2) ◽

pp. A165-A165 ◽

Cited By ~ 1

Author(s):

Shanxiang Zhang ◽

Mehdi Nassiri ◽

Lanting Liu ◽

Jihong Sun ◽

Ryan Stohler ◽

...

Keyword(s):

In Situ Hybridization ◽

B Cell ◽

Fluorescence In Situ Hybridization ◽

Cell Morphology ◽

Cell Lymphoma ◽

B Cell Lymphoma ◽

Signet Ring Cell ◽

Signet Ring ◽

Ring Cell

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Detection of Trisomy 3 in Primary Gastric B-Cell Lymphoma by Using Chromosome In Situ Hybridization on Paraffin Sections

American Journal of Clinical Pathology ◽

10.1093/ajcp/110.3.347 ◽

1998 ◽

Vol 110 (3) ◽

pp. 347-353 ◽

Cited By ~ 7

Author(s):

You Zhang ◽

Annie N. Y. Cheung ◽

Alexander C. L. Chan ◽

Dan-Hua Shen ◽

Wei-Sheng Xu ◽

...

Keyword(s):

In Situ Hybridization ◽

B Cell ◽

Cell Lymphoma ◽

B Cell Lymphoma ◽

Paraffin Sections ◽

Trisomy 3

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Immuno–Fluorescence In Situ Hybridization Index Predicts Survival in Patients With Diffuse Large B-Cell Lymphoma Treated With R-CHOP: A GELA Study

Journal of Clinical Oncology ◽

10.1200/jco.2009.22.7058 ◽

2009 ◽

Vol 27 (33) ◽

pp. 5573-5579 ◽

Cited By ~ 93

Author(s):

Christiane Copie-Bergman ◽

Philippe Gaulard ◽

Karen Leroy ◽

Josette Briere ◽

Maryse Baia ◽

...

Keyword(s):

In Situ Hybridization ◽

B Cell ◽

Fluorescence In Situ Hybridization ◽

Germinal Center ◽

Cell Lymphoma ◽

B Cell Lymphoma ◽

Immunohistochemical Markers ◽

Large B Cell Lymphoma ◽

Large B Cell

Purpose To evaluate the prognostic value of cell of origin immunohistochemical markers and BCL2, BCL6, and c-MYC translocations in a homogeneous cohort of patients with diffuse large B-cell lymphoma (DLBCL) treated with rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP). Patients and Methods Patients with CD20+ DLBCL were enrolled in the randomized LNH98-5 and 01-5B Groupe d'Etude des Lymphomes de l'Adulte trials. Paraffin-embedded tumor samples of 119 patients treated with R-CHOP were analyzed by immunohistochemistry for CD10, BCL6, MUM1/IRF4, LMO2, and forkhead box protein P1 (FOXP1) expression and for BCL2, BCL6, and c-MYC breakpoints by fluorescence in situ hybridization (FISH) on tissue microarray. Results LMO2 expression and BCL2 breakpoint were associated with the germinal center (GC) subtype defined by Hans' algorithm, respectively (P < .0001; P = .0002) whereas FOXP1 expression and BCL6 breakpoint were associated with the non-germinal center (non-GC) subtype (P = .008 and P = .0001, respectively). The immunohistochemical markers analyzed independently, GC/non-GC phenotype and BCL2 breakpoint did not predict overall survival (OS). BCL6 breakpoint was significantly associated with an unfavorable impact on OS (P = .04). Interestingly, an immunoFISH index, defined by positivity for at least two of three non-GC markers (FOXP1, MUM1/IRF4, BCL6 breakpoint) was significantly associated with a shorter 5-year OS rate (44%; 95% CI, 28 to 60 v 78%; 95% CI, 59 to 89; P = .01) which was independent (P = .04) of the age-adjusted International Prognostic Index (P = .04) in multivariate analysis. Conclusion Our study demonstrates that combining immunohistochemistry with FISH allows construction of an immunoFISH index that significantly predicts survival in elderly DLBCL patients treated with R-CHOP.

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Faculty Opinions recommendation of Immuno-fluorescence in situ hybridization index predicts survival in patients with diffuse large B-cell lymphoma treated with R-CHOP: a GELA study.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1165311.628262 ◽

2009 ◽

Author(s):

Michael Williams

Keyword(s):

In Situ Hybridization ◽

B Cell ◽

Fluorescence In Situ Hybridization ◽

Cell Lymphoma ◽

B Cell Lymphoma ◽

Large B Cell Lymphoma ◽

Gela Study ◽

Large B Cell ◽

Hybridization Index

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Detection of t(11;18)(q21;q21) by interphase fluorescence in situ hybridization using API2 and MLTspecific probes

Blood ◽

10.1182/blood.v96.6.2215.h8002215_2215_2218 ◽

2000 ◽

Vol 96 (6) ◽

pp. 2215-2218 ◽

Cited By ~ 1

Author(s):

Judith Dierlamm ◽

Mathijs Baens ◽

Margarita Stefanova-Ouzounova ◽

Kristina Hinz ◽

Iwona Wlodarska ◽

...

Keyword(s):

In Situ Hybridization ◽

B Cell ◽

Fluorescence In Situ Hybridization ◽

Cell Lymphoma ◽

B Cell Lymphoma ◽

Low Grade ◽

Chromosome 11 ◽

Interphase Nuclei ◽

Large B Cell

The translocation of chromosome 11, long arm, region 2, band 1, to chromosome 18, long arm, region 2, band 1 (t(11;18)(q21;q21)) represents a recurrent chromosomal abnormality in extranodal marginal zone B-cell lymphoma (MZBCL) of mucosa-associated lymphoid tissue (MALT) type and leads to a fusion of the apoptosis inhibitor-2 (API2) gene on chromosome 11 and the MALT lymphoma-associated translocation (MLT) gene on chromosome 18. A 2-color fluorescence in situ hybridization (FISH) assay, which can be used for the detection of t(11;18) in interphase nuclei and metaphase chromosomes on fresh and archival tumor tissue, was developed. The P1 artificial chromosome (PAC) clone located immediately telomeric to the MLT gene and the PAC clone spanning the API2 gene were differentially labeled and used to visualize the derivative chromosome 11 resulting from t(11;18), as evident by the overlapping or juxtaposed red and green fluorescent signals. The assay was applied to interphase nuclei of 20 cases with nonmalignant conditions and 122 B-cell non-Hodgkin's lymphomas (NHLs). The latter group comprised 20 cases of nodal follicle center cell lymphoma and diffuse large B-cell NHL, 10 cases of gastric diffuse large B-cell lymphoma, 10 cases of hairy cell leukemia, and 82 cases of MZBCL (41 extranodal from various locations, 19 nodal, and 22 splenic MZBCL) including 35 cases with an abnormal karyotype, 2 of which revealed t(11;18). By interphase FISH, t(11;18) was detected in 8 gastrointestinal low-grade MALT-type lymphomas including the 2 cytogenetically t(11;18)+ cases. In the 8 t(11;18)+ cases, the FISH results were confirmed by reverse transcriptase–polymerase chain reaction (RT-PCR) usingAPI2 and MLT specific primers. Our results indicate that t(11;18)(q21;q21) specifically characterizes a subgroup of low-grade MZBCL of the MALT-type and that the FISH assay described here is a highly specific and rapid test for the detection of this translocation.

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