scholarly journals The influence of DNA size on the binding of antibodies to DNA in the sera of normal human subjects and patients with systemic lupus erythematosus (SLE)

1999 ◽  
Vol 116 (2) ◽  
pp. 354-359 ◽  
Author(s):  
PISETSKY ◽  
GONZALEZ
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Human Subjects ◽  
Systemic Lupus ◽  
Normal Human ◽  
Dna Size ◽  
Antibodies To Dna

Antibodies to DNA in patients with systemic lupus erythematosus. Their role in the diagnosis, the follow-up and the pathogenesis of the disease

1990 ◽  
Vol 9 (S1) ◽  
pp. 100-110 ◽  
Author(s):  
R. Smeenk ◽  
K. Brinkman ◽  
H. Van Den Brink ◽  
R. -M. Termaat ◽  
J. Berden ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Systemic Lupus ◽  
Antibodies To Dna

Antibodies to native DNA: detection by immunoperoxidase assay and determination of their immunoglobulin classes.

1979 ◽  
Vol 25 (3) ◽  
pp. 366-370 ◽  
Author(s):  
A O Vladutiu ◽  
D A Palumbo ◽  
J E Asirwatham
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Antinuclear Antibodies ◽  
Immunoperoxidase Technique ◽  
Nuclear Staining ◽  
Systemic Lupus ◽  
Double Stranded Dna ◽  
Crithidia Luciliae ◽  
Farr Assay ◽  
Antibodies To Dna

Abstract Antinuclear antibodies are almost always found in sera of patients with systemic lupus erythematosus. To differentiate antinuclear antibodies from antibodies to DNA in the recently described Crithidia luciliae assay, we developed an immunoperoxidase technique for detecting antibodies to native, double-stranded DNA and compared results by it with those by the Farr assay. Smears of cultured Crithidia luciliae were incubated with human sera, peroxidase-labeled anti-human IgG serum, and diaminobenzidine. The peroxidase stain was examined by conventional light microscopy, which facilitated differentiation between the kinetoplast and the nuclear staining. The Crithidia assay appeared to be specific for double-stranded DNA antibodies, seemed to be more sensitive than the Farr assay, and allowed us to determine the immunoglobulin classes of antibodies to native DNA. Some patients with systemic lupus erythematosus had only IgM or IgA antibodies to DNA.

scholarly journals DNA receptor dysfunction in systemic lupus erythematosus and kindred disorders. Induction by anti-DNA antibodies, antihistone antibodies, and antireceptor antibodies.

1987 ◽  
Vol 166 (4) ◽  
pp. 850-863 ◽  
Author(s):  
R M Bennett ◽  
B L Kotzin ◽  
M J Merritt
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Intact Cell ◽  
Connective Tissue Diseases ◽  
Systemic Lupus ◽  
Humoral Factors ◽  
Receptor Cells ◽  
Marked Inhibition ◽  
Dna Antibodies ◽  
Antibodies To Dna

The ability of sera from patients with SLE and similar connective tissue diseases to induce dysfunction of the receptor for DNA was studied. All SLE and MCTD sera studied resulted in marked inhibition of DNA receptor binding. Furthermore, the sera from a subgroup of patients with other rheumatic diseases and a surprisingly high percentage of asymptomatic relatives of SLE patients exhibited a similar effect. The humoral factors causing this defect were shown to be of at least three reactivities: (a) antibodies to DNA, (b) antibodies to histones, and (c) antibodies to the DNA receptor itself. The reactivity of anti-DNA and antihistone antibodies is dependent upon intact cell-surface DNA, and reconstitution experiments suggest that antihistone antibodies are reactive with histones complexed to this DNA, which in turn is bound to the DNA receptor. Cells with an antibody-induced DNA receptor defect are unable to bind DNA; the subsequent inability to degrade DNA may have important consequences in diseases such as SLE in which DNA-anti-DNA immune complexes are of pathogenetic significance.

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