Morphology, pathogenicity and isozyme variation amongst French isolatesof Leptosphaeria maculans recovered from Brassica juncea cv. Picra

1996 ◽  
Vol 45 (6) ◽  
pp. 1090-1098 ◽  
Author(s):  
I. SOMDA ◽  
M. RENARD ◽  
H. BRUN
Keyword(s):  
Brassica Juncea ◽  
Leptosphaeria Maculans ◽  
Isozyme Variation

Mapping genes for resistance to Leptosphaeria maculans in Brassica juncea

Genome ◽  
2006 ◽  
Vol 49 (1) ◽  
pp. 30-41 ◽  
Author(s):  
J A Christianson ◽  
S R Rimmer ◽  
A G Good ◽  
D J Lydiate
Keyword(s):  
Linkage Group ◽  
Brassica Juncea ◽  
Fragment Length Polymorphism ◽  
Leptosphaeria Maculans ◽  
Recessive Gene ◽  
Brassica Species ◽  
Blackleg Disease ◽  
Recessive Resistance ◽  
B Genome ◽  
Restriction Fragment Length

Blackleg disease of crucifers, caused by the fungus Leptosphaeria maculans, is a major concern to oilseed rape producers worldwide. Brassica species containing the B genome have high levels of resistance to blackleg. Brassica juncea F2 and first-backcross (B1) populations segregating for resistance to a PG2 isolate of L. maculans were created. Segregation for resistance to L. maculans in these populations suggested that resistance was controlled by two independent genes, one dominant and one recessive in nature. A map of the B. juncea genome was constructed using segregation in the F2 population of a combination of restriction fragment length polymorphism (RFLP) and microsatel lite markers. The B. juncea map consisted of 325 loci and was aligned with previous maps of the Brassica A and B genomes. The gene controlling dominant resistance to L. maculans was positioned on linkage group J13 based on segregation for resistance in the F2 population. This position was confirmed in the B1 population in which the resistance gene was definitively mapped in the interval flanked by pN199RV and sB31143F. The provisional location of the recessive gene controlling resistance to L. maculans on linkage group J18 was identified using a subset of informative F2 individuals.Key words: blackleg, B genome, phoma, recessive resistance.

scholarly journals Impact of Cruciferous Phytoalexins on the Detoxification of Brassilexin by the Blackleg Fungus Pathogenic to Brown Mustard

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500
Author(s):  
M. Soledade C. Pedras ◽  
Ryan B. Snitynsky
Keyword(s):  
Brassica Juncea ◽  
Leptosphaeria Maculans ◽  
Fungal Species ◽  
Important Group ◽  
Blackleg Fungus

The biotransformation of brassilexin, a potent phytoalexin produced by brown mustard (Brassica juncea L.), in the presence of various cruciferous phytoalexins was investigated. An important group of isolates of the fungal species Leptosphaeria maculans (Laird 2 and Mayfair 2), which is virulent to brown mustard, but not to canola, was used in this investigation. Brassilexin was detoxified by the fungus, but none of the phytoalexins seemed to affect substantially the rate of brassilexin detoxification; after 12 h of incubation, the amounts of brassilexin remaining in culture were as low as in controls, except in co-incubations with cyclobrassinin and sinalexin, which afforded intermediates that in solution oxidized spontaneously to brassilexin.

Centennial Brown brown condiment mustard

2009 ◽  
Vol 89 (2) ◽  
pp. 337-340 ◽  
Author(s):  
G. Rakow ◽  
J. P. Raney ◽  
D. Rode ◽  
J. Relf-Eckstein
Keyword(s):  
Grain Yield ◽  
Brassica Juncea ◽  
Seed Quality ◽  
Leptosphaeria Maculans ◽  
Research Centre ◽  
Glucosinolate Content ◽  
Market Place ◽  
Improvement Program ◽  
Canadian Prairies ◽  
White Rust

Brown condiment mustard (Common Brown) has about 10% lower grain yield than oriental condiment mustard (yellow seeded), which both belong to the same species [Brassica juncea (L.) Czern.]. Yield improvements in brown condiment mustard are therefore of great importance. The Saskatoon Research Centre of AAFC initiated a condiment brown mustard improvement program in 1996 applying pedigree selection of single plants from the condiment brown mustard cultivar Blaze, which resulted in the selection and registration of the cultivar Centennial Brown. Centennial Brown yielded 3.2% more grain than the landrace Common Brown, on average over 81 location years in 9 yr of condiment mustard Co-op tests (1999–2007) and was well adapted to the mustard-growing areas of the Canadian prairies. Support for registration was based on 6 yr of Co-op tests. Centennial Brown had the same maturity (91 d) and was 5 cm taller (116 cm) than Common Brown. It had 1.5% lower fixed oil (36.6%) and 1.2% greater protein content (30.0%) compared with Common Brown. It had 0.4 g heavier seed (2.96 g 1000 seed-1) than Common Brown. Centennial Brown had 0.9 mg g seed-1 greater allyl glucosinolate content than Common Brown (9.15 mg g seed-1). Green seed counts were low in Centennial Brown (0.64%) compared with Common Brown (0.79%). This was confirmed in chlorophyll content measurements, 4.76 mg kg-1 for Centennial Brown and 5.24 mg kg-1 for Common Brown. Centennial Brown was resistant to blackleg disease [Leptosphaeria maculans (Desm.) Ces. et de Not.] and highly susceptible to the B. juncea races of white rust [Albugo candida (Pers.) Kuntze], equal to Common Brown. Centennial Brown will quickly replace Common Brown in the market place because of its increased grain yield and much superior seed quality. Key words: Brassica juncea (L.) Czern., cultivar description, grain yield, seed quality

scholarly journals Inheritance of Resistance to Leptosphaeria maculans in Brassica juncea

1997 ◽  
Vol 87 (6) ◽  
pp. 594-598 ◽  
Author(s):  
M. Keri ◽  
C. G. J. van den Berg ◽  
P. B. E. McVetty ◽  
S. R. Rimmer
Keyword(s):  
Brassica Juncea ◽  
Gene Action ◽  
Leptosphaeria Maculans ◽  
Causal Agent ◽  
Nuclear Genes ◽  
Recessive Trait ◽  
Inheritance Of Resistance ◽  
Susceptible Parent ◽  
Inoculation Methods ◽  
Inoculation Method

The inheritance of resistance to Leptosphaeria maculans, the causal agent of black leg of crucifers, was studied in Brassica juncea. Three resistant accessions (UM3021, UM3043, and UM3323) and one susceptible accession (UM3132) of B. juncea were crossed in a complete diallel. Parents, F1, and F2 progenies were evaluated for all crosses using both cotyledon and stem inoculation. Cotyledon reaction was evaluated with two isolates of L. maculans, but stem reaction was evaluated with one isolate. Disease reactions observed for individual plants were the same for both inoculation methods and for both isolates of the pathogen for cotyledon reaction. No segregation was observed for the crosses between resistant accessions (UM3043 × UM3323 and UM3021 × UM3323), but a few susceptible plants were observed in the F2 progeny of crosses between resistant parents (UM3021 × UM3043). This was probably due to heterozygosity in some parental plants of UM3021. For crosses be tween the susceptible parent and resistant parents, F1 plants for two crosses were all resistant. For cross UM3132 × UM3021, some susceptible plants occurred, which was also suggestive of heterozygosity in UM3021. Although resistance in F1 was dominant, for F2 populations, segregation fit either 13:3, 3:1, or 1:3 ratios, indicating that resistance can be either adominant or recessive trait. F3 families derived from some susceptible F2 plants from crosses UM3021 × UM3132 and UM3043 × UM3132 were evaluated using the cotyledon inoculation method only. Segregation of F2 plants and F3 families in crosses involving resistant and susceptible parents indicated that the resistance to L. maculans in B. juncea is controlled by two nuclear genes with dominant recessive epistatic gene action.

Acantozero erucic acid oriental condiment mustard

2010 ◽  
Vol 90 (4) ◽  
pp. 499-502
Author(s):  
G. Rakow ◽  
J P Raney ◽  
D. Rode
Keyword(s):  
Erucic Acid ◽  
Brassica Juncea ◽  
Seed Oil ◽  
Seed Protein ◽  
Leptosphaeria Maculans ◽  
Glucosinolate Content ◽  
Blackleg Disease ◽  
Albugo Candida ◽  
White Rust ◽  
Cultivar Description

Acanto is the first zero erucic acid oriental condiment mustard [Brassica juncea (L.) Czern.]. It has the same grain yield, plant height, seed protein content, seed weight, allyl glucosinolate content, and seed chlorophyll content as the check cultivar Cutlass. Both cultivars are highly resistant to blackleg disease caused by Leptosphaeria maculans (Desm.) Ces et de Not., but are susceptible to white rust race 2v caused by Albugo candida (Pers.) Kuntze. Acanto is more susceptible to white rust race 2a than is Cutlass. Acanto matures 2 d later than Cutlass, has a 2.9% lower seed oil (fixed oil) content and its seed colour is a darker yellow. Acanto was well adapted to the mustard growing areas of the Canadian prairies.Key words: Brassica juncea (L.) Czern., cultivar description, zero erucic acid oil

Sign in / Sign up

Export Citation Format

Share Document