scholarly journals Immunolabelling, histochemistry and in situ hybridisation in human skeletal muscle fibres to detect myosin heavy chain expression at the protein and mRNA level

2001 ◽  
Vol 199 (3) ◽  
pp. 329-337 ◽  
Author(s):  
A. L. SERRANO ◽  
MARGARITA PÉREZ ◽  
A. LUCÍA ◽  
J. L. CHICHARRO ◽  
E. QUIROZ-ROTHE ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Mrna Level ◽  
In Situ Hybridisation ◽  
Muscle Fibres ◽  
Skeletal Muscle Fibres

Co-existence of myosin heavy chain I and IIa isoforms in human skeletal muscle fibres with endurance training

1990 ◽  
Vol 416 (4) ◽  
pp. 470-472 ◽  
Author(s):  
H. Klitgaard ◽  
O. Bergman ◽  
R. Betto ◽  
G. Salviati ◽  
S. Schiaffino ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Endurance Training ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Muscle Fibres ◽  
Skeletal Muscle Fibres

Three myosin heavy chain isoforms in type 2 skeletal muscle fibres

1989 ◽  
Vol 10 (3) ◽  
pp. 197-205 ◽  
Author(s):  
Stefano Schiaffino ◽  
Luisa Gorza ◽  
Saverio Sartore ◽  
Leopoldo Saggin ◽  
Simonetta Ausoni ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Muscle Fibres ◽  
Skeletal Muscle Fibres

Two functionally distinct myosin heavy chain isoforms in slow skeletal muscle fibres

FEBS Letters ◽  
1997 ◽  
Vol 410 (2-3) ◽  
pp. 150-152 ◽  
Author(s):  
Stefan Galler ◽  
Karlheinz Hilber ◽  
Bärbel Gohlsch ◽  
Dirk Pette
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Muscle Fibres ◽  
Slow Skeletal Muscle ◽  
Skeletal Muscle Fibres

Myosin Heavy Chain Transitions in Dog Skeletal Muscle Fibres during Post-natal Development

2005 ◽  
Vol 34 (s1) ◽  
pp. 50-50
Author(s):  
M. Strbenc ◽  
V. Smerdu ◽  
N. Tozon ◽  
M. Vrecl ◽  
M. Ursic ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Muscle Fibres ◽  
Skeletal Muscle Fibres

Myosin heavy chain isoforms and velocity of shortening of type 2 skeletal muscle fibres

1998 ◽  
Vol 134 (4) ◽  
pp. 575-576 ◽  
Author(s):  
S. SCHIAFFINO ◽  
S. AUSONI ◽  
L. GORZA ◽  
L. SAGGIN ◽  
K. GUNDERSEN ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Muscle Fibres ◽  
Velocity Of Shortening ◽  
Skeletal Muscle Fibres

Myosin heavy chain isoforms and velocity of shortening of type 2 skeletal muscle fibres

1988 ◽  
Vol 134 (4) ◽  
pp. 575-576 ◽  
Author(s):  
S. SCHIAFFINO ◽  
S. AUSONI ◽  
L. GORZA ◽  
L. SAGGIN ◽  
K. GUNDERSEN ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Muscle Fibres ◽  
Velocity Of Shortening ◽  
Skeletal Muscle Fibres

Mismatch between myosin heavy chain mRNA and protein distribution in human skeletal muscle fibers

1997 ◽  
Vol 272 (6) ◽  
pp. C1881-C1889 ◽  
Author(s):  
J. L. Andersen ◽  
S. Schiaffino
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Relative Proportion ◽  
Mrna Levels ◽  
Protein Distribution ◽  
Slow Type ◽  
A Minor

The distribution of myosin heavy chain (MHC) isoforms was analyzed at the protein and mRNA levels in human skeletal muscle biopsies from young normal adult subjects. Using ATPase histochemical reactions, antibodies to fast- and slow-type MHCs, and in situ hybridization with probes specific for MHC-beta/slow, MHC-2A, and MHC-2X, we confirmed our previous results showing that most fibers contain either a single mRNA and isoprotein or a mixed 1/2A or 2A/2X phenotype with coexistence of two mRNAs and isoproteins. However, we also found a minor proportion of fibers showing a mismatch in the relative proportion of mRNA and protein, e.g., fibers containing MHC-2A mRNA but not the corresponding protein or fibers containing MHC-2A protein but not the corresponding transcript. These fibers were more frequent in biopsies obtained after a training or detraining period than before the training period. We propose that these fibers represent transitional fibers and that the relative content of each mRNA and isoprotein gives a clue as to the direction of change in MHC gene expression.

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