Characterization of the blood-brain barrier choline transporter using the in situ rat brain perfusion technique

2001 ◽  
Vol 77 (2) ◽  
pp. 704-704
Author(s):  
David D. Allen ◽  
Quentin R. Smith
Keyword(s):  
Rat Brain ◽  
Blood Brain Barrier ◽  
Brain Perfusion ◽  
Brain Barrier ◽  
Perfusion Technique ◽  
Choline Transporter ◽  
Blood Brain

Investigation of Transport Mechanism of Pentazocine across the Blood‐Brain Barrier Using the In Situ Rat Brain Perfusion Technique

2002 ◽  
Vol 91 (11) ◽  
pp. 2346-2353 ◽  
Author(s):  
Toyofumi Suzuki ◽  
Masakazu Oshimi ◽  
Kazuo Tomono ◽  
Manabu Hanano ◽  
Jun Watanabe
Keyword(s):  
Rat Brain ◽  
Blood Brain Barrier ◽  
Transport Mechanism ◽  
Brain Perfusion ◽  
Brain Barrier ◽  
Perfusion Technique ◽  
Blood Brain

scholarly journals Comparison of blood-brain barrier permeability in mice and rats using in situ brain perfusion technique

2000 ◽  
Vol 279 (3) ◽  
pp. H1022-H1028 ◽  
Author(s):  
Hideyasu Murakami ◽  
Hitomi Takanaga ◽  
Hirotami Matsuo ◽  
Hisakazu Ohtani ◽  
Yasufumi Sawada
Keyword(s):  
Blood Brain Barrier ◽  
Brain Perfusion ◽  
External Carotid Artery ◽  
Brain Barrier ◽  
External Carotid ◽  
Perfusion Technique ◽  
Perfusion Rate ◽  
In Situ Brain Perfusion ◽  
Blood Brain

Here we present a method for measuring the permeability coefficient-surface area product ( PS) values at the blood-brain barrier in mice, using the in situ brain perfusion technique originally developed for rats by Takasato et al. ( Am J Physiol Heart Circ Physiol 247: H484–H493, 1984). Retrograde infusion into the right external carotid artery increased the carotid perfusion pressure in proportion to the perfusion rate. Intravascular volume and cerebral perfusion fluid flow at a perfusion rate of 1.0 ml/min in mice were similar to those in rats. In addition, the contribution of systemic blood to total flow in the hemisphere was small (only 3.2%). These findings indicated that this perfusion rate is suitable for mice. The PS values of more than 20 different compounds were determined in mice by using the in situ brain perfusion technique, and comparisons were made with data from rats. There was a close relationship (1:1) between the PS values in mice and rats, indicating that brain capillary permeabilities are similar in mice and rats.

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