Covalent Linkage of Recombinant Hirudin to a Novel Ionic Poly(Carbonate) Urethane Polymer with Protein Binding Sites: Determination of Surface Antithrombin Activity

1998 ◽  
Vol 22 (8) ◽  
pp. 657-665 ◽  
Author(s):  
Matthew D. Phaneuf ◽  
Michael Szycher ◽  
Scott A. Berceli ◽  
Donald J. Dempsey ◽  
William C. Quist ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Recombinant Hirudin ◽  
Covalent Linkage ◽  
Protein Binding Sites ◽  
Antithrombin Activity ◽  
Poly Carbonate

13C NMR Method for the Determination of Peptide and Protein Binding Sites in Lipid Bilayers and Emulsions

2001 ◽  
Vol 105 (50) ◽  
pp. 12616-12621 ◽  
Author(s):  
Emiko Okamura ◽  
Tomohiro Kimura ◽  
Masaru Nakahara ◽  
Masafumi Tanaka ◽  
Tetsurou Handa ◽  
...  
Keyword(s):  
Protein Binding ◽  
Lipid Bilayers ◽  
13C Nmr ◽  
Binding Sites ◽  
Protein Binding Sites ◽  
Nmr Method

EVALUATION OF TISSUE STEROID BINDING IN VITRO

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S223-S246 ◽  
Author(s):  
C. R. Wira ◽  
H. Rochefort ◽  
E. E. Baulieu
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Experimental System ◽  
Specific Protein ◽  
Coupling Mechanism ◽  
Target Tissue ◽  
Protein Binding Sites ◽  
Definition Of ◽  
Hormone Specificity

ABSTRACT The definition of a RECEPTOR* in terms of a receptive site, an executive site and a coupling mechanism, is followed by a general consideration of four binding criteria, which include hormone specificity, tissue specificity, high affinity and saturation, essential for distinguishing between specific and nonspecific binding. Experimental approaches are proposed for choosing an experimental system (either organized or soluble) and detecting the presence of protein binding sites. Techniques are then presented for evaluating the specific protein binding sites (receptors) in terms of the four criteria. This is followed by a brief consideration of how receptors may be located in cells and characterized when extracted. Finally various examples of oestrogen, androgen, progestagen, glucocorticoid and mineralocorticoid binding to their respective target tissues are presented, to illustrate how researchers have identified specific corticoid and mineralocorticoid binding in their respective target tissue receptors.

scholarly journals Transcription signals and protein binding sites for sericin gene transcription in vitro

1989 ◽  
Vol 264 (31) ◽  
pp. 18707-18713 ◽  
Author(s):  
K Matsuno ◽  
C C Hui ◽  
S Takiya ◽  
T Suzuki ◽  
K Ueno ◽  
...  
Keyword(s):  
Protein Binding ◽  
Gene Transcription ◽  
Binding Sites ◽  
Protein Binding Sites ◽  
Transcription In Vitro ◽  
Transcription Signals
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