In recent years, in order to minimize traffic accidents, developing driving assistance systems for security has attracted much attention. Lane detection is an essential element of avoiding accidents and enhancing driving security. In this chapter, the authors implement a novel real-time lighting-invariant lane departure warning system. The proposed methodology works well in different lighting conditions, such as in poor conditions. The experimental results and accuracy evaluation indicates the efficiency of the system proposed for lane detection. The correct detection rate averages 97% and exceeds 95.6% in poor conditions. Furthermore, the entire process has only 29 ms per frame.