Cardiovascular, Haematological and Biochemical Responses After Large Volume Blood Collection in Horses

Abstract Background and study aims Endoscopic ultrasound (EUS) has been used for portal vein sampling in patients with pancreaticobiliary cancers for enumerating circulating tumor cells but is not yet a standard procedure. Further evaluation is needed to refine the methodology. Therefore, we evaluated the feasibility and safety of 19-gauge (19G) versus a 22-gauge (22 G) EUS fine-needle aspiration needles for portal vein sampling in a swine model. Methods Celiotomy was performed on two farm pigs. Portal vein sampling occurred transhepatically. We compared 19 G and 22 G needles coated interiorly with saline, heparin or ethylenediaminetetraacetic acid (EDTA). Small- (10 mL) and large- (25 mL) volume blood collections were evaluated. Two different collection methods were tested: direct-to-vial and suction syringe. A bleeding risk trial for saline-coated 19 G and 22 G needles was performed by puncturing the portal vein 20 times. Persistent bleeding after 3 minutes was considered significant. Results All small-volume collection trials were successful except for 22 G saline-coated needles with direct-to-vial method. All large-volume collection trials were successful when using suction syringe; direct-to-vial method for both 19 G and 22 G needles were unsuccessful. Collection times were shorter for 19 G vs. 22 G needles for both small and large-volume collections (P < 0.05). Collection times for saline-coated 22 G needles were longer compared to heparin/EDTA-coated (P < 0.05). Bleeding occurred in 10 % punctures with 19 G needles compared to 0 % with 22 G needles. Conclusion The results of this animal study demonstrate the feasibility and the safety of using 22 G needles for portal vein sampling and can form the basis for a pilot study in patients.

Download Full-text

Risk scores to facilitate preoperative prediction of transfusion and large volume blood transfusion associated with adult cardiac surgery †

British Journal of Anaesthesia ◽

10.1093/bja/aeu483 ◽

2015 ◽

Vol 114 (5) ◽

pp. 757-766 ◽

Cited By ~ 39

Author(s):

R. Goudie ◽

J.A.C. Sterne ◽

V. Verheyden ◽

M. Bhabra ◽

M. Ranucci ◽

...

Keyword(s):

Cardiac Surgery ◽

Blood Transfusion ◽

Large Volume ◽

Risk Scores ◽

Preoperative Prediction ◽

Volume Blood ◽

Adult Cardiac Surgery

Download Full-text

Risk Scores to Facilitate Preoperative Prediction of Transfusion and Large Volume Blood Transfusion Associated With Adult Cardiac Surgery

Survey of Anesthesiology ◽

10.1097/01.sa.0000480664.54971.53 ◽

2016 ◽

Vol 60 (2) ◽

pp. 45-46

Author(s):

R. Goudie ◽

J. A. C. Sterne ◽

V. Verheyden ◽

M. Bhabra ◽

M. Ranucci ◽

...

Keyword(s):

Cardiac Surgery ◽

Blood Transfusion ◽

Large Volume ◽

Risk Scores ◽

Preoperative Prediction ◽

Volume Blood ◽

Adult Cardiac Surgery

Download Full-text

A method for large volume blood sampling and transfusion in rats

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1986.250.3.e331 ◽

1986 ◽

Vol 250 (3) ◽

pp. E331-E337

Author(s):

H. R. Berthoud ◽

W. B. Laughton ◽

T. L. Powley

Keyword(s):

Large Volume ◽

Whole Blood ◽

Superior Vena ◽

Vena Cava ◽

Blood Sampling ◽

Indwelling Catheters ◽

Multiple Channel ◽

Glucose Levels ◽

Include Blood Pressure ◽

Volume Blood

A new protocol that makes it feasible to withdraw large volumes of whole blood from an individual rat within 1 h or less is described. This method involves the use of indwelling catheters for withdrawal of blood from the inferior vena cava with concurrent isovolemic replacement of whole blood into the superior vena cava. Simultaneity of the transfusion and withdrawal, strict equality of volumes, and a smooth exchange of blood are assured by the use of separate channels of the same multiple-channel peristaltic pump for withdrawal and replacement. Validation experiments using both anesthetized and unanesthetized rats indicate that several responses remain essentially undisturbed during large volume blood sampling; these parameters include blood pressure, heart rate, hematocrit, plasma hormones including insulin and glucagon, plasma glucose levels, and feeding behavior. Considerations of technical and physiological limitations of the protocol, including choice of catheters and catheter placement, pump, sampling parameters, and obtaining donor blood, are discussed.

Download Full-text