Neurotransmitter release from bovine adrenal chromaffin cells is modulated by capacitative Ca2+entry driven by depleted internal Ca2+stores

Objective. Phosphoinositides play a regulatory role in clathrin-mediated endocytosis. However, their involvement in clathrin-independent endocytosis termed rapid endocytosis (RE), which is the mode of vesicle recycling during neurotransmitter release by transient fusion (known as kiss-and-run), has not been investigated. Here, we used patch-clamp recording of whole-cell membrane capacitance in adrenal chromaffin cells (ACC) to monitor changes of RE kinetics in response to pharmacological alteration of phosphatidylinositol-4,5-biphosphate (PI(4,5)P2) level by phenylarsine oxide (PAO) or antibody against phosphatidylinositol 4-kinase (AbPI4K). Results. We found that PAO and AbPI4K significantly abrogated RE kinetics. Infusion of PI(4,5)P2 through the patch pipette potentiated RE kinetics and reversed PAO- and AbPI4K-induced blockade of RE. Similarly, the application of the bifunctional thiol dithiothreitol (DTT) to PAO-treated cells completely prevented the inhibitory effect of PAO on RE. These findings indicate that PI(4,5)P2 is implicated in the signaling (mechanistic) process of RE in ACC.

Download Full-text

Difference in neurotransmitter release induced by calcium channel and acetylcholine receptor activation in adrenal chromaffin cells

Journal of Physiology-Paris ◽

10.1016/j.jphysparis.2005.12.050 ◽

2006 ◽

Vol 99 (2-3) ◽

pp. 1

Author(s):

Elena A. Lukyanetz ◽

Oleg M. Pochynyuk ◽

Oleg L. Zaika ◽

Larisa M. Koval ◽

Elena N. Yavorskaya ◽

...

Keyword(s):

Calcium Channel ◽

Acetylcholine Receptor ◽

Neurotransmitter Release ◽

Chromaffin Cells ◽

Receptor Activation ◽

Adrenal Chromaffin Cells ◽

Adrenal Chromaffin

Download Full-text

Spatio-Temporal Regulation of Neurotransmitter Release by PKC; Studies in Adrenal Chromaffin Cells

Critical Reviews in Neurobiology ◽

10.1615/critrevneurobiol.v16.i12.180 ◽

2004 ◽

Vol 16 (1-2) ◽

pp. 173-179 ◽

Cited By ~ 5

Author(s):

Konosuke Kumakura ◽

Nobuyiki Sasakawa ◽

Norie Murayama ◽

Mica Ohara-Imaizumi

Keyword(s):

Neurotransmitter Release ◽

Chromaffin Cells ◽

Adrenal Chromaffin Cells ◽

Temporal Regulation ◽

Spatio Temporal ◽

Adrenal Chromaffin

Download Full-text

Morphological Observations on the Granule Types in Rabbit Extra-Adrenal Chromaffin Cells.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100115213 ◽

1975 ◽

Vol 33 ◽

pp. 318-319

Author(s):

Joe A. Mascorro ◽

Robert D. Yates

Keyword(s):

Chromaffin Cells ◽

Sodium Phosphate ◽

Ganglion Cells ◽

Ultrastructural Level ◽

Osmic Acid ◽

Adrenal Chromaffin Cells ◽

Potassium Iodate ◽

Perfusion Fluid ◽

New Zealand White Rabbits ◽

Adrenal Chromaffin

Extra-adrenal chromaffin organs (abdominal paraganglia) constitute rich sources of catecholamines. It is believed that these bodies contain norepinephrine exclusively. However, the present workers recently observed epinephrine type granules in para- ganglion cells. This report investigates catecholamine containing granules in rabbit paraganglia at the ultrastructural level.New Zealand white rabbits (150-170 grams) were anesthetized with 50 mg/kg Nembutal (IP) and perfused with 3% glutaraldehyde buffered with 0.2M sodium phosphate, pH 7.3. The retroperitoneal tissue blocks were removed and placed in perfusion fluid for 4 hours. The abdominal paraganglia were dissected from the blocks, diced, washed in phosphate buffer and fixed in 1% osmic acid buffered with phosphate. In other animals, the glutaraldehyde perfused tissue blocks were immersed for 1 hour in 3% glutaraldehyde/2.5% potassium iodate buffered as before. The paraganglia were then diced, separated into two vials and washed in the buffer. A portion of this tissue received osmic acid fixation.

Download Full-text