Difference in neurotransmitter release induced by calcium channel and acetylcholine receptor activation in adrenal chromaffin cells

2006 ◽  
Vol 99 (2-3) ◽  
pp. 1
Author(s):  
Elena A. Lukyanetz ◽  
Oleg M. Pochynyuk ◽  
Oleg L. Zaika ◽  
Larisa M. Koval ◽  
Elena N. Yavorskaya ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Acetylcholine Receptor ◽  
Neurotransmitter Release ◽  
Chromaffin Cells ◽  
Receptor Activation ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

scholarly journals Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P2) Is Required for Rapid Endocytosis in Chromaffin Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Fouad Azizi
Keyword(s):  
Neurotransmitter Release ◽  
Chromaffin Cells ◽  
Inhibitory Effect ◽  
Adrenal Chromaffin Cells ◽  
Patch Clamp Recording ◽  
Vesicle Recycling ◽  
Patch Pipette ◽  
Cell Membrane Capacitance ◽  
Adrenal Chromaffin ◽  
Phosphatidylinositol 4

Objective. Phosphoinositides play a regulatory role in clathrin-mediated endocytosis. However, their involvement in clathrin-independent endocytosis termed rapid endocytosis (RE), which is the mode of vesicle recycling during neurotransmitter release by transient fusion (known as kiss-and-run), has not been investigated. Here, we used patch-clamp recording of whole-cell membrane capacitance in adrenal chromaffin cells (ACC) to monitor changes of RE kinetics in response to pharmacological alteration of phosphatidylinositol-4,5-biphosphate (PI(4,5)P2) level by phenylarsine oxide (PAO) or antibody against phosphatidylinositol 4-kinase (AbPI4K). Results. We found that PAO and AbPI4K significantly abrogated RE kinetics. Infusion of PI(4,5)P2 through the patch pipette potentiated RE kinetics and reversed PAO- and AbPI4K-induced blockade of RE. Similarly, the application of the bifunctional thiol dithiothreitol (DTT) to PAO-treated cells completely prevented the inhibitory effect of PAO on RE. These findings indicate that PI(4,5)P2 is implicated in the signaling (mechanistic) process of RE in ACC.

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