scholarly journals Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System

Planta Medica ◽  
2021 ◽  
Author(s):  
Deborah Spiess ◽  
Vanessa Fabienne Abegg ◽  
Antoine Chauveau ◽  
Andrea Treyer ◽  
Michael Reinehr ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Lactate Production ◽  
Glucose Consumption ◽  
Positive Control ◽  
Placental Perfusion ◽  
Perfusion Model ◽  
Beta Human Chorionic Gonadotropin ◽  
Pathologic Findings ◽  
Age Appropriate ◽  
Placental Passage

AbstractThe placental passage of humulone and protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Only a small portion of humulone initially present in the maternal circuit reached the fetal circuit. The humulone concentration in the maternal circuit rapidly decreased, likely due to metabolization in the placenta. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. None of the study compounds affected placental viability or functionality, as glucose consumption, lactate production, beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings.

Ex Vivo Human Placental Perfusion Model for Analysis of Fetal Circulation in the Chorionic Plate

2016 ◽  
Vol 35 (3) ◽  
pp. 553-560 ◽  
Author(s):  
Zoya Gordon ◽  
Lilia Glaubach ◽  
David Elad ◽  
Uri Zaretsky ◽  
Ariel J. Jaffa
Keyword(s):  
Ex Vivo ◽  
Placental Perfusion ◽  
Fetal Circulation ◽  
Perfusion Model ◽  
Chorionic Plate

Utilization of an ex vivo human placental perfusion model to predict potential fetal exposure to carboplatin during pregnancy

2014 ◽  
Vol 210 (3) ◽  
pp. 275.e1-275.e9 ◽  
Author(s):  
Judith A. Smith ◽  
Anjali Gaikwad ◽  
Scott Mosley ◽  
Larry Coffer ◽  
Jeffrey Cegelski ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Fetal Exposure ◽  
Placental Perfusion ◽  
Perfusion Model

Placental transfer of bromocriptine in an ex vivo human placental perfusion model

2017 ◽  
Vol 32 (7) ◽  
pp. 1155-1159 ◽  
Author(s):  
Qiaoling Zheng ◽  
Qiong Zhou ◽  
Juan Li ◽  
Yuqin Tian ◽  
Hua Huang ◽  
...  
Keyword(s):  
Placental Transfer ◽  
Ex Vivo ◽  
Placental Perfusion ◽  
Perfusion Model

Ex vivo human placental perfusion model for analysis of feto-placental vasculature

Placenta ◽  
2017 ◽  
Vol 57 ◽  
pp. 272 ◽  
Author(s):  
David Elad ◽  
Uri Zaretsky ◽  
Zoya Gordon ◽  
Ariel Jaffa
Keyword(s):  
Ex Vivo ◽  
Placental Perfusion ◽  
Perfusion Model

The effect of aspirin on placental vessels reactivity using the ex-vivo placental perfusion model

2018 ◽  
Vol 170 ◽  
pp. 84-86
Author(s):  
Michal Kovo ◽  
Miriam Rubinchik-Stern ◽  
Hadas Miremberg ◽  
Oded Luria ◽  
Inna Bliecher ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Placental Perfusion ◽  
Perfusion Model

77: Maternal transfer of Interleukin-6 in the ex-vivo placental perfusion model

2007 ◽  
Vol 197 (6) ◽  
pp. S34 ◽  
Author(s):  
Kathryn Villano ◽  
Roxanne Holt ◽  
Michael Zaretsky ◽  
Roger Bawdon
Keyword(s):  
Interleukin 6 ◽  
Ex Vivo ◽  
Maternal Transfer ◽  
Placental Perfusion ◽  
Perfusion Model

Placental Transfer of Pyrimethamine Studied in an ex vivo Placental Perfusion Model

Neonatology ◽  
2000 ◽  
Vol 78 (2) ◽  
pp. 83-85 ◽  
Author(s):  
Gilles Peytavin ◽  
Jean-Joël Leng ◽  
François Forestier ◽  
Marie-Claude Saux ◽  
Patrick Hohlfeld ◽  
...  
Keyword(s):  
Placental Transfer ◽  
Ex Vivo ◽  
Placental Perfusion ◽  
Perfusion Model

scholarly journals Transfer of Meropenem in the ex Vivo Human Placenta perfusion Model

2005 ◽  
Vol 13 (4) ◽  
pp. 223-227 ◽  
Author(s):  
Michael Hnat ◽  
Roger E. Bawdon
Keyword(s):  
Ex Vivo ◽  
Maternal Serum ◽  
Transplacental Passage ◽  
Maternal Circulation ◽  
Placental Perfusion ◽  
Perfusion Model ◽  
Serum Peak ◽  
Trough Concentrations ◽  
The Mean ◽  
Trough Levels

Objectives.To determine maternal-fetal transplacental passage of meropenem in the ex vivo human perfusion model.Study design.Term placentae (n= 6) were collected immediately after delivery. A single cotyledon was localized, perfused and stabilized with physiologic Eagles minimal essential medium containing 3% bovine albumin and heparin as described by Chalier (Chalier JC. Criteria for evaluating perfusion experiments and presentation results. Contrib Gynecol Obstet 1985; 13:32–39). Meropenem was added to the maternal medium in concentrations similar to maternal serum peak and trough levels, then perfused through the maternal circulation of the cotyledon. To assess transfer and accumulation, fluid aliquots from both the maternal and fetal compartments were collected over an hour at defined intervals in an open and closed system. AntipyrineC14was added to the medium in order to calculate the transport fraction and clearance indexes. Meropenem and antipyrineC14concentrations were determined by High-pressure Liquid Chromatography and liquid scintillation, respectively.Results.Mean antipyrine transport fraction was 2.33 + 0.25. Maternal and fetal mean meropenem peak concentrations were 54.3 + 3.3μg/ml and 2.2 + 0.18μg/ml, respectively. Whereas, maternal and fetal mean trough concentrations were 12.7 + 1.3μg/ml and 0.41 + 0.10μg/ml, respectively. Mean peak clearance index was 0.077 + 0.007 and the mean trough was 0.052 + 0.015. Mean accumulation for the peak and trough concentrations of meropenem were 0.9 and 2.95μg/ml, respectively.Conclusions.Transplacental passage of meropenem was incomplete in the ex vivo human placental perfusion model. Accumulation was also noted in the fetal compartment

Trypanasoma cruzi invades placental cells in a human ex vivo placental perfusion model

2003 ◽  
Vol 189 (6) ◽  
pp. S88
Author(s):  
Stuart Shippey ◽  
Christopher Zahn ◽  
T.John Wu ◽  
Margaret Cisar ◽  
Robert Gherman ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Placental Perfusion ◽  
Perfusion Model ◽  
Placental Cells
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