A Simple, Low-Cost Computer Program for Scatchard Plot Analysis of Binding Data in Steriod Hormone Receptor Assays

Abstract. To determine the effect of steroid occupancy of the glucocorticoid receptor on the measurement of total receptor concentration using Scatchard plot of [3H]dexamethasone (Dex) binding to circulating leukocytes, leukocytes were incubated with 10−7m cortisol or 10−9m Dex prior to [3H]Dex binding study. Total binding capacity calculated from Scatchard plot analysis of [3H]Dex binding was not significantly reduced after pre-incubation with cortisol. However, total binding capacity was significantly lower after pre-incubation with Dex. These data suggest that total glucocorticoid receptor concentration can be measured from a 3 h incubation of leukocytes obtained from untreated patients.

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Binding of mitochondrial malate dehydrogenase to mitoplasts

Canadian Journal of Biochemistry ◽

10.1139/o79-083 ◽

1979 ◽

Vol 57 (6) ◽

pp. 662-665 ◽

Cited By ~ 6

Author(s):

Paula M. Strasberg ◽

Keith A. Webster ◽

Hasmukh V. Patel ◽

Karl B. Freeman

Keyword(s):

Malate Dehydrogenase ◽

Rat Liver ◽

Specific Binding ◽

Liver Mitochondria ◽

Scatchard Plot ◽

Rat Liver Mitochondria ◽

Isolated Mitochondria ◽

Plot Analysis ◽

Scatchard Plot Analysis ◽

Mitochondrial Malate Dehydrogenase

The binding of 14C-labelled bovine and porcine malate dehydrogenase (EC 1.1.1.37) to rat liver mitochondria and mitoplasts was examined. The bovine enzyme was found to associate nonspecifically with isolated mitochondria and sonicated mitoplasts. Scatchard plot analysis suggested a specific binding to mitoplasts of the order of 5 pmol malate dehydrogenase per milligram of mitoplast protein. Porcine malate dehydrogenase dimer but not monomer exhibited a similar binding. The results are discussed in relation to the mechanism of uptake of the enzyme by mitochondria after synthesis on cytosolic ribosomes.

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Oestrogen receptors in endometrial cytosol of gilts on days 10-13 of oestrous cycle and pregnancy

Netherlands Journal of Agricultural Science ◽

10.18174/njas.v42i3.599 ◽

1994 ◽

Vol 42 (3) ◽

pp. 217-223

Author(s):

J. Van Der Meulen ◽

F.A. Helmond ◽

C.P.J. Oudenaarden

Keyword(s):

Oestrous Cycle ◽

Endometrial Tissue ◽

Scatchard Plot ◽

Oestrogen Receptors ◽

Reproductive State ◽

Dextran Coated Charcoal ◽

Plot Analysis ◽

Scatchard Plot Analysis

Cytosolic oestrogen receptors (ER(c)) were determined with a Dextran-coated charcoal assay and Scatchard plot analysis in endometrial tissue of non-pregnant and pregnant gilts on Days 10-13 after standing oestrus. The Kd of the ER(c) was 0.40 +/- 0.04 nM (mean +/- SEM) and was not affected by day or reproductive state. The endometrial ER(c) concentration was affected by day and reproductive state (P

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Hormone-receptor Assays and Cancer of the Breast: The Pathologist’s Role

American Journal of Clinical Pathology ◽

10.1093/ajcp/70.5.719 ◽

1978 ◽

Vol 70 (5) ◽

pp. 719-720 ◽

Cited By ~ 1

Author(s):

Joseph H. Keffer

Keyword(s):

Hormone Receptor ◽

Receptor Assays

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Multidisciplinary Approach to Neoadjuvant Endocrine Therapy in Breast Cancer: A Comprehensive Review

Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics ◽

10.1055/s-0036-1597579 ◽

2016 ◽

Vol 38 (12) ◽

pp. 615-622 ◽

Cited By ~ 7

Author(s):

Tomás Reinert ◽

Susana Ramalho ◽

Rodrigo Gonçalves ◽

Carlos Barrios ◽

Marcia Graudenz ◽

...

Keyword(s):

Breast Cancer ◽

Endocrine Therapy ◽

Low Income ◽

Hormone Receptor ◽

Urban Areas ◽

Low Cost ◽

Locally Advanced Breast Cancer ◽

Locally Advanced ◽

Breast Cancers ◽

Neoadjuvant Endocrine Therapy

AbstractBreast cancer is the most common type of cancer and the leading cause of cancer-related death among women worldwide. Hormone receptor-positive (HR+) tumors represent the most common form of this disease, with more than 70% of breast cancers expressing these receptors. Response and benefit to neoadjuvant chemotherapy (NCT) varies according to HR expression, with lower responses in luminal tumors as compared with hormone receptor-negative (HR-) and human epidermal growth factor receptor 2-positive (HER2+) tumors. Neoadjuvant endocrine therapy (NET) is an option for selected patients with HR+ locally advanced breast cancer. Neoadjuvant endocrine therapy has a favorable toxicity profile, and is associated with benefits such as having low cost and being more easily available even for cancer care professionals outside major urban areas or tertiary centers. These factors are particularly relevant, as 70% of breast cancer deaths occur in women from low-income and middle-income countries. Additionally, NET is being increasingly explored, not simply to allow for less extensive surgery, but also as a scientific tool, with the use of biomarkers to predict outcomes in adjuvant trials and for the individual patient. This review details the current and most relevant evidence about NET for breast cancer as well as the future directions of this field.

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