Sorption behavior of Cu(II) ions on insolubilized humic acid under acidic conditions: An application of Scatchard plot analysis in evaluating the pH dependence of specific and nonspecific bindings

Abstract. To determine the effect of steroid occupancy of the glucocorticoid receptor on the measurement of total receptor concentration using Scatchard plot of [3H]dexamethasone (Dex) binding to circulating leukocytes, leukocytes were incubated with 10−7m cortisol or 10−9m Dex prior to [3H]Dex binding study. Total binding capacity calculated from Scatchard plot analysis of [3H]Dex binding was not significantly reduced after pre-incubation with cortisol. However, total binding capacity was significantly lower after pre-incubation with Dex. These data suggest that total glucocorticoid receptor concentration can be measured from a 3 h incubation of leukocytes obtained from untreated patients.

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Binding of mitochondrial malate dehydrogenase to mitoplasts

Canadian Journal of Biochemistry ◽

10.1139/o79-083 ◽

1979 ◽

Vol 57 (6) ◽

pp. 662-665 ◽

Cited By ~ 6

Author(s):

Paula M. Strasberg ◽

Keith A. Webster ◽

Hasmukh V. Patel ◽

Karl B. Freeman

Keyword(s):

Malate Dehydrogenase ◽

Rat Liver ◽

Specific Binding ◽

Liver Mitochondria ◽

Scatchard Plot ◽

Rat Liver Mitochondria ◽

Isolated Mitochondria ◽

Plot Analysis ◽

Scatchard Plot Analysis ◽

Mitochondrial Malate Dehydrogenase

The binding of 14C-labelled bovine and porcine malate dehydrogenase (EC 1.1.1.37) to rat liver mitochondria and mitoplasts was examined. The bovine enzyme was found to associate nonspecifically with isolated mitochondria and sonicated mitoplasts. Scatchard plot analysis suggested a specific binding to mitoplasts of the order of 5 pmol malate dehydrogenase per milligram of mitoplast protein. Porcine malate dehydrogenase dimer but not monomer exhibited a similar binding. The results are discussed in relation to the mechanism of uptake of the enzyme by mitochondria after synthesis on cytosolic ribosomes.

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A Simple, Low-Cost Computer Program for Scatchard Plot Analysis of Binding Data in Steriod Hormone Receptor Assays

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-0029-1210448 ◽

2009 ◽

Vol 85 (03) ◽

pp. 263-268 ◽

Cited By ~ 1

Author(s):

G. Kind ◽

K. Pollow ◽

H.-J. Grill ◽

B. Manz ◽

A. Heubner

Keyword(s):

Computer Program ◽

Hormone Receptor ◽

Low Cost ◽

Scatchard Plot ◽

Plot Analysis ◽

Binding Data ◽

Scatchard Plot Analysis ◽

Receptor Assays

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Oestrogen receptors in endometrial cytosol of gilts on days 10-13 of oestrous cycle and pregnancy

Netherlands Journal of Agricultural Science ◽

10.18174/njas.v42i3.599 ◽

1994 ◽

Vol 42 (3) ◽

pp. 217-223

Author(s):

J. Van Der Meulen ◽

F.A. Helmond ◽

C.P.J. Oudenaarden

Keyword(s):

Oestrous Cycle ◽

Endometrial Tissue ◽

Scatchard Plot ◽

Oestrogen Receptors ◽

Reproductive State ◽

Dextran Coated Charcoal ◽

Plot Analysis ◽

Scatchard Plot Analysis

Cytosolic oestrogen receptors (ER(c)) were determined with a Dextran-coated charcoal assay and Scatchard plot analysis in endometrial tissue of non-pregnant and pregnant gilts on Days 10-13 after standing oestrus. The Kd of the ER(c) was 0.40 +/- 0.04 nM (mean +/- SEM) and was not affected by day or reproductive state. The endometrial ER(c) concentration was affected by day and reproductive state (P

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A simple computer program for Scatchard plot analysis of steroid receptors including non-specific binding correction on a low cost desk top calculator

Journal of Steroid Biochemistry ◽

10.1016/0022-4731(79)90363-7 ◽

1979 ◽

Vol 11 (5-6) ◽

pp. 1641-1646 ◽

Cited By ~ 30

Author(s):

S. Schwarz

Keyword(s):

Computer Program ◽

Steroid Receptors ◽

Specific Binding ◽

Low Cost ◽

Scatchard Plot ◽

Simple Computer ◽

Plot Analysis ◽

Scatchard Plot Analysis ◽

Simple Computer Program

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Influence of Humic Acid on Sorption of Neptunium(V) onto Soil

MRS Proceedings ◽

10.1557/proc-353-997 ◽

1994 ◽

Vol 353 ◽

Cited By ~ 5

Author(s):

Y. Sakamoto ◽

S. Nagao ◽

T. Ohnuki ◽

M. Senoo ◽

A. Ohashi ◽

...

Keyword(s):

Humic Acid ◽

Organic Carbon ◽

Stability Constant ◽

Dissolved Organic Carbon ◽

Size Distribution ◽

Ph Dependence ◽

Soil Layer ◽

Sorption Behavior ◽

Migration Behavior ◽

The Stability

AbstractThe pH dependence of the stability constant of Np(V)-humate and the sorption of Np(V) onto soil was studied to clarify the influence of dissolved organic carbon on the migration behavior of Np(V) in soil layer. The stability constant of Np(V)-humate was expressed by logβ1=(0.35 ±0.03)pH+0.04±0.01 in the pH region from 5.3 to 8.7, and the intrinsic stability constant by logβ*1=3.66±0.05.The sorption of Np(V) increased with pH up to pH 7, and tended to reach maximum, about 95%, at pH 9 in the absence of humic acid. In the presence of humic acid more than 140mg/l, the sorption of Np(V) was lower than that in the absence of humic acid above pH 7, while any influence of humic acid on the sorption of Np(V) onto soil was not observed below pH 7. The sorption behavior of Np(V) onto soil was explained by reversible sorption of NpO2+ and sorption of NpO2CO3- and/or NpO2OH. Further, the influence of the size distribution of Np(V) on the sorption onto soil was examined and discussed.

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