The Possible Potassium Involvement in the Modulation of the Long-Term Trophic Action of ACTH on the Rat Adrenal Zona Fasciculata

ABSTRACT The effect of 3′:5′-cyclic AMP (cAMP) on the incorporation of 3H-uridine into adrenocortical cells of hypophysectomized rats was investigated by high resolution autoradiography. The quantitative analysis of autoradiographs shows that cAMP, like ACTH, enhances the tracer incorporation into both nuclei and mitochondria. These findings are discussed in relation to the results of various investigations, indicating that cAMP functions as an intracellular mediator of the long-term trophic action of ACTH on the adrenal cortex. It is suggested that the mechanism of this action of cAMP consists in the stimulation of both nuclear and mitochondrial RNA synthesis. Furthermore, the possibility that the trophic action of ACTH on the adrenal gland involves other intracellular mediators, as well as cAMP, is suggested.

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Chromatin Fractal Organization, Textural Patterns, and Circularity of Nuclear Envelope in Adrenal Zona Fasciculata Cells

Microscopy and Microanalysis ◽

10.1017/s1431927616011910 ◽

2016 ◽

Vol 22 (6) ◽

pp. 1120-1127 ◽

Cited By ~ 4

Author(s):

Igor Pantic ◽

Dejan Nesic ◽

Milos Basailovic ◽

Mila Cetkovic ◽

Sanja Mazic ◽

...

Keyword(s):

Fractal Dimension ◽

Nuclear Envelope ◽

Current Knowledge ◽

Significant Negative Correlation ◽

Computer Assisted ◽

Cell Physiology ◽

Zona Fasciculata ◽

Research Software ◽

Occurrence Matrix ◽

Adrenal Zona Fasciculata

AbstractDespite previous research efforts in the fields of histology and cell physiology, the relationship between chromatin structural organization and nuclear shape remains unclear. The aim of this research was to test the existence and strength of correlations between mathematical parameters of chromatin microarchitecture and roundness of the nuclear envelope. On a sample of 240 nuclei of adrenal zona fasciculata cells stained using the DNA-specific Feulgen method, we quantified fractal parameters such as fractal dimension and lacunarity, as well as textural parameters such as angular second moment (ASM), entropy, inverse difference moment, contrast, and variance. Circularity of the nuclear envelope was determined from the nuclear area and perimeter. The results indicate that there is a statistically significant negative correlation between chromatin ASM and circularity. Moreover, there was a statistically significant positive correlation between chromatin fractal dimension and envelope circularity. This is the first study to demonstrate these relationships in adrenal tissue, and also one of the first studies to test the connection between circularity and fractal and gray-level co-occurrence matrix parameters in DNA-specific Feulgen stain. The results could be useful both as an addition to the current knowledge on chromatin/nuclear envelope interactions, and for design of future computer-assisted research software for evaluation of nuclear morphology.

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