Developmental exposure to endocrine disrupter dichlorodiphenyltrichloroethane alters transcriptional regulation of postnatal morphogenesis of adrenal zona fasciculata

The capacity of the adrenal to produce steroids is controlled in part through the transcriptional regulation of steroid enzymes. The orphan nuclear receptor steroidogenic factor 1 (SF-1) is central to the transcriptional regulation of all steroid hydroxylase enzymes, whereas nur77 can preferentially regulate steroid enzyme genes relevant to cortisol production. We hypothesised that, in the presence of secretagogues, SF-1 and nur77 may differentially interact with coregulatory proteins in the human adrenal cortex. Both coregulatory proteins, steroid receptor coactivator (SRC-1) and silencing mediator for retinoid and thyroid hormones (SMRT), were found to be expressed in the zona fasciculata and reticularis in the human adrenal cortex, but were largely absent from the zona glomerulosa. Both coregulatory proteins were colocalised with SF-1 and nur77. In the H295R adrenal tumour cell line, SF-1 and nur77 transcripts were increased in cells in the presence of forskolin, whereas nur77 mRNA was also induced with angiotensin II (AII). The coactivator SRC-1 mRNA was increased in the presence of both forskolin and AII. Forskolin induced recruitment of SRC-1 to the SF-1 response element and induced SRC-1–SF-1 interactions, whereas AII increased recruitment of SRC-1 to the nur77 response element and induced SRC-1–nur77 interactions. The corepressor SMRT interacted with SF-1 in the presence of AII and with nur77 in cells treated with forskolin. Orphan nuclear receptor–coregulatory protein interactions may have consequences for the regulation of key steroidogenic enzymes in the human adrenal cortex.

Download Full-text

PROLONGED INTERLEUKIN-1β ADMINISTRATION STIMULATES THE GROWTH OF THE RAT ADRENAL ZONA FASCICULATA

Biomedical Research ◽

10.2220/biomedres.12.259 ◽

1991 ◽

Vol 12 (4) ◽

pp. 259-262 ◽

Cited By ~ 4

Author(s):

ALDONA KASPRZAK ◽

PIERA REBUFFAT ◽

VIRGILIO MENEGHELLI ◽

GIUSEPPINA MAZZOCCHI ◽

GASTONE G. NUSSDORFER

Keyword(s):

Interleukin 1Β ◽

Zona Fasciculata ◽

Adrenal Zona Fasciculata

Download Full-text

Effects of mevinolin, an inhibition of cholesterol synthesis, on the morphological and functional responses of rat adrenal zona fasciculata to a prolonged treatment with 4-aminopyrazolo-pyrimidine

Biology of the Cell ◽

10.1016/0248-4900(88)90194-3 ◽

1988 ◽

Vol 63 (S1) ◽

pp. 23-23a

Author(s):

Giuseppina Mazzocchi ◽

AnnaS. Belloni ◽

Piera Rebuffat ◽

GastoneG. Nussdorfer

Keyword(s):

Cholesterol Synthesis ◽

Prolonged Treatment ◽

Zona Fasciculata ◽

Functional Responses ◽

Adrenal Zona Fasciculata

Download Full-text

Chromatin Fractal Organization, Textural Patterns, and Circularity of Nuclear Envelope in Adrenal Zona Fasciculata Cells

Microscopy and Microanalysis ◽

10.1017/s1431927616011910 ◽

2016 ◽

Vol 22 (6) ◽

pp. 1120-1127 ◽

Cited By ~ 4

Author(s):

Igor Pantic ◽

Dejan Nesic ◽

Milos Basailovic ◽

Mila Cetkovic ◽

Sanja Mazic ◽

...

Keyword(s):

Fractal Dimension ◽

Nuclear Envelope ◽

Current Knowledge ◽

Significant Negative Correlation ◽

Computer Assisted ◽

Cell Physiology ◽

Zona Fasciculata ◽

Research Software ◽

Occurrence Matrix ◽

Adrenal Zona Fasciculata

AbstractDespite previous research efforts in the fields of histology and cell physiology, the relationship between chromatin structural organization and nuclear shape remains unclear. The aim of this research was to test the existence and strength of correlations between mathematical parameters of chromatin microarchitecture and roundness of the nuclear envelope. On a sample of 240 nuclei of adrenal zona fasciculata cells stained using the DNA-specific Feulgen method, we quantified fractal parameters such as fractal dimension and lacunarity, as well as textural parameters such as angular second moment (ASM), entropy, inverse difference moment, contrast, and variance. Circularity of the nuclear envelope was determined from the nuclear area and perimeter. The results indicate that there is a statistically significant negative correlation between chromatin ASM and circularity. Moreover, there was a statistically significant positive correlation between chromatin fractal dimension and envelope circularity. This is the first study to demonstrate these relationships in adrenal tissue, and also one of the first studies to test the connection between circularity and fractal and gray-level co-occurrence matrix parameters in DNA-specific Feulgen stain. The results could be useful both as an addition to the current knowledge on chromatin/nuclear envelope interactions, and for design of future computer-assisted research software for evaluation of nuclear morphology.

Download Full-text