scholarly journals Effects of Asymmetric Dynamic Loading on Intervertebral Disc – towards a Scoliosis Mimicking Organ Culture Model

2016 ◽  
Vol 6 (1_suppl) ◽  
pp. s-0036-1582636-s-0036-1582636
Author(s):  
Zhen Li ◽  
Ying Zhang ◽  
Lukas Straumann ◽  
Patrick Lezuo ◽  
Marianna Peroglio ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Dynamic Loading ◽  
Culture Model

Effects of Caffeine on Intervertebral Disc Cell Viability in a Whole Organ Culture Model

2017 ◽  
Vol 17 (10) ◽  
pp. S125-S126
Author(s):  
Benjamin T. Raines ◽  
James T. Stannard ◽  
Olivia E. Stricklin ◽  
Aaron M. Stoker ◽  
Theodore J. Choma ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Cell Viability ◽  
Disc Cell ◽  
Culture Model

scholarly journals 178 AN ORGAN CULTURE MODEL TO STUDY BIOLOGICAL REPAIR OF THE DEGENERATE INTERVERTEBRAL DISC

2009 ◽  
Vol 17 ◽  
pp. S104-S105
Author(s):  
L.A. Haglund ◽  
J. Moir ◽  
B. Jim ◽  
P.J. Roughley ◽  
T. Steffen
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Biological Repair ◽  
Culture Model

Development of a Large Animal Long-Term Intervertebral Disc Organ Culture Model That Includes the Bony Vertebrae for Ex Vivo Studies

2016 ◽  
Vol 22 (7) ◽  
pp. 636-643 ◽  
Author(s):  
Michael Grant ◽  
Laura M. Epure ◽  
Omar Salem ◽  
Nizar AlGarni ◽  
Ovidiu Ciobanu ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Ex Vivo ◽  
Large Animal ◽  
Culture Model

scholarly journals Assessment of the Matrix Degenerative Effects of MMP-3, ADAMTS-4, and HTRA1, Injected Into a Bovine Intervertebral Disc Organ Culture Model

Spine ◽  
2013 ◽  
Vol 38 (22) ◽  
pp. E1377-E1387 ◽  
Author(s):  
Tina Furtwängler ◽  
Samantha C. W. Chan ◽  
Gregor Bahrenberg ◽  
Peter J. Richards ◽  
Benjamin Gantenbein-Ritter
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture Model ◽  
The Matrix

scholarly journals An In Vitro Organ Culture Model of the Murine Intervertebral Disc

10.3791/55437 ◽  
2017 ◽  
Author(s):  
Jennifer W. Liu ◽  
Kevin H. Lin ◽  
Christian Weber ◽  
Sameer Bhalla ◽  
Sean Kelso ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture Model

scholarly journals Detrimental effects of discectomy on intervertebral disc biology can be decelerated by growth factor treatment during surgery: a large animal organ culture model

2014 ◽  
Vol 14 (11) ◽  
pp. 2724-2732 ◽  
Author(s):  
Svenja Illien-Jünger ◽  
Young Lu ◽  
Devina Purmessur ◽  
Jillian E. Mayer ◽  
Benjamin A. Walter ◽  
...  
Keyword(s):  
Growth Factor ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Large Animal ◽  
Culture Model ◽  
Animal Organ ◽  
Growth Factor Treatment

scholarly journals Development of a whole organ culture model for intervertebral disc disease

2016 ◽  
Vol 5 ◽  
pp. 1-8 ◽  
Author(s):  
James T. Stannard ◽  
Kazuya Edamura ◽  
Aaron M. Stoker ◽  
Grace D. O'Connell ◽  
Keiichi Kuroki ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Intervertebral Disc Disease ◽  
Disc Disease ◽  
Culture Model

scholarly journals A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues

2015 ◽  
Vol 201 (1) ◽  
pp. 38-50 ◽  
Author(s):  
Zhengjian Yan ◽  
Liangjun Yin ◽  
Zhongliang Wang ◽  
Jixing Ye ◽  
Zhonglin Zhang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Disc Degeneration ◽  
Bovine Serum ◽  
Recombinant Adenovirus ◽  
Complex Structure ◽  
Central Nucleus ◽  
Culture Model ◽  
Fetal Bovine

The intervertebral disc (IVD) is a fibrocartilaginous joint between two vertebral bodies. An IVD unit consists of a gelatinous central nucleus pulposus, encased by the annulus fibrosus, which is sandwiched between cartilaginous endplates (EPs). The IVD homeostasis can be disrupted by injuries, ageing and/or genetic predispositions, leading to degenerative disc disorders and subsequent lower back pain. The complex structure and distinct characteristics of IVDs warrant the establishment of robust in vitro IVD organ culture for studying the etiology and treatment of disc degeneration. Here, we isolate mouse lumbar IVDs and culture the minimal IVD units in submersion or suspension medium supplemented with 2% bovine serum or 10% fetal bovine serum (FBS). We find the minimal IVD units remain healthy for up to 14 days when cultured in submersion culture supplemented with 10% FBS. New bone formation in the EPs of the cultured IVDs can be assessed with calcein labeling. Furthermore, the cultured IVDs can be effectively transduced by recombinant adenovirus, and transgene expression lasts for 2 weeks. Thus, our findings demonstrate that the optimized IVD organ culture system can be used to study IVD biology and screen for biological factors that may prevent, alleviate and/or treat disc degeneration.

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