Natural Oestrogen in the Female Climacteric - Influence on Blood Coagulation and Fibrinolysis

SummaryThe aim of the present study was to investigate the effect on blood coagulation and fibrinolysis of a natural oestrogen preparation, piperazine oestrone sulphate, prospectively in menopausal women. Scopolamine was given to the control group.The women were investigated before and during treatment with regard to factors VIII, VII, X, V, fibrinopeptide A, antithrombin III, plasminogen, rapid antiplasmin and α1-antitrypsin. There was no significant change towards hypercoagulability or decreased fibrinolysis in any group. In the oestrogen group, however, a tendency towards an increased level of plasminogen and a decreased level of antiplasmin was demonstrated. In the scopolamine group there was an unexpected fall in factors X and V and also in plasminogen and α1,-antitrypsin. A low level of some blood coagulation factors in some of the women before treatment is somewhat astonishing; none of them had any history of excessive bleeding.

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EXPRESSION IN ONTOGENESIS OF HUMAN BLOOD COAGULATION FACTORS

10.1055/s-0038-1644610 ◽

1987 ◽

Author(s):

H J Hassan ◽

A Leonardi ◽

C Chelucci ◽

R Guerriero ◽

P M Mannucci ◽

...

Keyword(s):

Blood Coagulation ◽

Protein C ◽

Antithrombin Iii ◽

Liver Homogenate ◽

Coagulation Factor ◽

Coagulation Factors ◽

Factor Ix ◽

Adult Liver ◽

Blood Coagulation Factors ◽

Guanidine Isothiocyanate

We have analyzed the expression of several blood coagulation factors (IX, VIII, X, fibrinogen chains) and inhibitors (antithrombin III, protein C) in human embryonic and fetal livers, obtained from legal abortions at 6-11 week post-conception. The age was established by morphologic staging and particularly crown-rump lenght measurement.Total cellular RNA was isolated from partially purified hepatocytes or total liver homogenate using the guanidine isothiocyanate method. Poly(A)+ RNA was selected by oligodT cellulose chromatography. The size and the number of the embryonic and fetal transcripts are equivalent to those observed in adult liver, as evaluated by Northern blot analysis of total or poly(A)+ RNA hybridized to human cDNA probes.The level of coagulation factor transcripts in embryonic and fetal liver was evaluated by dot hybridization of total RNA (0.5-10 ug), as compared to RNA extracted from normal adult liver biopsies. The expression of blood coagulation factors in embryos is generally reduced for all factors, but at a different degree. In 5-11 wk liver, the level of factor IX is 5-10% of that observed in adults, while fibrinogen, protein C, antithrombin III RNA level rises from 25 to 50% and factor X is expressed at a level comparable to that observed in adult liver.We conclude that during these stages of development blood coagulation factors are expressed according to three different time, curves, possibly due to the effect of different types of regulatory mechanisms.

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Coagulation Factors of the Newborn and His Mother

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648106 ◽

1973 ◽

Vol 29 (03) ◽

pp. 644-651 ◽

Cited By ~ 13

Author(s):

L Biland ◽

F Duckert

Keyword(s):

Cord Blood ◽

Blood Coagulation ◽

Factor Xiii ◽

Antithrombin Iii ◽

Coagulation Factors ◽

Viii And Ix ◽

Birth Time ◽

Coagulation And Fibrinolysis

SummaryIn 50 mothers and their newborns 13 parameters of blood coagulation and fibrinolysis have been investigated. No correlation was found between the activity in the mother’s blood and in the cord blood.The activity of the factors V, VIII and IX can be so low in the blood of the normal newborn that it is not possible to detect a hereditary coagulopathy early at birth time. Fibrinogen and factor XIII make an exception. The factor XIII is normal in the cord blood, even higher than in the mother’s blood.The values of fibrinogen measured with different methods disagree (Table 1). These results are discussed in connection with the antithrombin II, antithrombin III, split products and plasminogen values.

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Abnormal Properties and Behaviors of Antithrombin III Found in a Thrombophilic Patient: Defective Biological Functions and Dissimilar Antigenic Determinants

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661633 ◽

1986 ◽

Vol 56 (02) ◽

pp. 165-171 ◽

Cited By ~ 3

Author(s):

Hideki Murayama ◽

Michio Matsuda

Keyword(s):

Blood Coagulation ◽

Antithrombin Iii ◽

Coagulation Factor ◽

Coagulation Factors ◽

Chain Structure ◽

Antigenic Determinants ◽

Factor X ◽

Single Chain ◽

Blood Coagulation Factors ◽

Genetically Determined

SummaryAbnormal properties of antithrombin III have been found in a 55-year-old male who has been thrombophilic over the last seven years. They are characterized by 1) defective inhibition of thrombin and activated blood coagulation factor X, 2) reduced affinity to heparin and 3) partial immunological identity with the normal molecule. The antithrombin III molecule, however, preserves a single-chain structure and an apparently identical molecular weight with that of the normal molecule. It is, thus, very unlikely that the impaired functions of antithrombin III in the patient’s plasma are induced by possible proteolytic modifications of the molecule by thrombin or other related activated blood coagulation factors.Since no other members of his immediate family have been found to be affected, the abnormality may be acquired rather than genetically determined, although further investigation is necessary for the elucidation of the abnormality of the molecule.

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Changes in Blood Coagulation and Fibrinolysis in Rats Fed Atherogenic Diets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654785 ◽

1963 ◽

Vol 10 (02) ◽

pp. 295-308 ◽

Cited By ~ 8

Author(s):

Clarence Merskey ◽

Herbert Wohl

Keyword(s):

Blood Coagulation ◽

Blood Platelets ◽

Coagulation Factors ◽

Atherogenic Diet ◽

Normal Serum ◽

Significant Prolongation ◽

Lysis Time ◽

Factor Ii ◽

Euglobulin Lysis Time ◽

Coagulation And Fibrinolysis

Summary1. Groups of rats were fed thrombogenic diets and the effects on blood coagulation and fibrinolysis assessed.2. Animals fed a diet containing cholesterol, thiouracil and cholic acid developed high levels of coagulation factors I, II, V, VII—X, VIII, IX and X.3. Animals fed a similar diet with additional 40% beef fat developed even greater elevation of V, VII—X, VIII and X, similar elevation of factor II, and lesser (but still significant) elevation of factors I and IX. In addition marked elevation of blood platelets occurred.4. Euglobulin lysis time of the group not fed the additional fat was longer than in controls. Significant prolongation of euglobulin lysis time was not found in the group fed additional fat.5. If the increased levels of plasma fibrinogen were taken into account, it was found that a larger amount of fibrin was lysed per unit time in the euglobulin lysis test with plasma from rats fed either atherogenic diet compared with controls.6. Defective thromboplastin generation was present in both groups of rats fed an atherogenic diet. The defect was present in the serum and was not due to lack of a factor required for thromboplastin generation. An inhibitor was present in the serum which was capable of preventing the action of normal serum.7. No good correlation was found between the occurrence of changes in blood coagulation or fibrinolysis and the presence or absence of thrombosis and infarction.8. The exact cause of these anomalies remains unexplained, as does the cause of the thrombosis in these animals. Starvation per se does not account for these abnormal findings. They could not adequately be explained on the basis of “hypercoagulability” of the blood.

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Factor II Related Antigen and Antithrombin III Levels as Indicators of Liver Failure in Consumption Coagulopathy

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657171 ◽

1982 ◽

Vol 47 (03) ◽

pp. 218-220 ◽

Cited By ~ 13

Author(s):

P Sié ◽

E Letrenne ◽

C Caranobe ◽

M Genestal ◽

B Cathala ◽

...

Keyword(s):

Liver Failure ◽

Antithrombin Iii ◽

Coagulation Factors ◽

Consumption Coagulopathy ◽

Blood Coagulation Factors ◽

Factor Ii ◽

At Iii ◽

Group A ◽

Group B

SummaryIn order to detect impaired synthesis of blood coagulation factors associated to consumption coagulopathy, a simultaneous evaluation of factor II-related antigen (II rAg) and of antithrombin III (AT III) was carried out in 16 patients affected with severe defibrination. An in vitro preliminary study on plasma and serum demonstrated that the levels of II rAg and of AT III, assessed by the Laurell technique with Behring antisera, were not reduced by the coagulation process. The patients were, a posteriori, classified into two groups according to the absence (group A) or the presence (group B) of factors predisposing to liver failure such as metastasis, cirrhosis, and prolonged shock. II rAg and AT III levels are significantly correlated; they are in the normal range in group A but reduced in group B. Thus II rAg or AT III level determinations are useful markers in the detection of liver failure associated to the consumption phenomenon. These results also suggest that part of the decreased AT III levels reported in severe cases of disseminated intravascular coagulation may be the consequence of an associated liver failure.

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STEROIDSTRUKTUR UND LEBERTOXIZITÄT

Acta Endocrinologica ◽

10.1530/acta.0.0540073 ◽

1967 ◽

Vol 54 (1) ◽

pp. 73-84 ◽

Cited By ~ 7

Author(s):

H. L. Krüskemper ◽

G. Noell

Keyword(s):

Alkaline Phosphatase ◽

Liver Function ◽

Blood Coagulation ◽

Electrophoretic Pattern ◽

Coagulation Factors ◽

Blood Coagulation Factors ◽

Methyl Group ◽

Liver Functions ◽

Male Subjects ◽

Androstane Derivatives

ABSTRACT In male subjects investigations have been carried out regarding the effect of C1- and C17-methylated androstane derivatives (20 mg per day, orally, two weeks) on liver functions (parameters: activities of GPT, GOT, alkaline phosphatase and cholinesterase in serum; electrophoretic pattern; blood coagulation factors V, VII, X and prothrombin; BSP-retention). In addition to the well known hepatotropic action of 17α-alkylated C-19-steroids a quasi-axial 1α-methyl configuration (in 1α-methylandrost-2-en-17β-ol) definitely increased BSP-retention and several coagulation factors. These steroid effects decreased gradually when a methyl group was introduced in C1 equatorially (1-methylandrost-1-en-17β-ol-3-one) or quasi-equatorially (1β-methylandrost-2-en-17β-ol), the latter compound completely lacking from any influence on parameters of liver function under investigation.

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