The results of an experimental study of the effect of dielectric barrier discharge (DBR) actuator on laminar-turbulent transition in a three-dimensional boundary layer under influence of elevated free-stream turbulence are presented. The travelling cross-flow instability modes are dominated in transition in a base configuration. Their characteristics do not depend on a spanwise position. The DBD-actuator that generated stationary cross-flow vortices with the predefined spanwise wavelength when turned on was capable to reduce a turbulent spots production rate in comparison to the base regime.