Three-dimensional reconstruction of the dynactin complex by single-particle image analysis

Two different three-dimensional reconstruction techniques for the shape of gas bubbles flowing in a liquid are presented. The first technique is based on the Dynamic Generalized Hough Transform Algorithm, and the second on the Metaball Model. These techniques are suitable for analysis of turbulent two-phase bubbly flows. Both techniques require at least two views of the bubble intended for three-dimensional reconstruction, and can be used in either stereoscopic or orthogonal camera setups. Once the reconstruction is accomplished, the bubble images can be accurately removed from the images acquired during Particle Image Velocimtery or Shadow Image Velocimetry measurements. After removing the bubble images from PIV images, a typical analysis of the liquid phase can be performed. This improves the accuracy of the statistical analysis of the parameters of each phase.

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Overabundant single-particle electron microscope views induce a three-dimensional reconstruction artifact

Ultramicroscopy ◽

10.1016/s0304-3991(98)00042-4 ◽

1998 ◽

Vol 74 (4) ◽

pp. 201-207 ◽

Cited By ~ 27

Author(s):

Nicolas Boisset ◽

Pawel A. Penczek ◽

Jean-Christophe Taveau ◽

Valérie You ◽

Felix de Haas ◽

...

Keyword(s):

Electron Microscope ◽

Single Particle ◽

Three Dimensional ◽

Three Dimensional Reconstruction ◽

Dimensional Reconstruction

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Faculty Opinions recommendation of Three-dimensional structure of transporter associated with antigen processing (TAP) obtained by single Particle image analysis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1003089.34205 ◽

2002 ◽

Author(s):

Tim Elliott

Keyword(s):

Image Analysis ◽

Single Particle ◽

Antigen Processing ◽

Particle Image ◽

Three Dimensional ◽

Dimensional Structure ◽

Three Dimensional Structure

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The interpretation of granitic textures from serial thin sectioning, image analysis and three-dimensional reconstruction

Mineralogical Magazine ◽

10.1180/minmag.1995.059.395.05 ◽

1995 ◽

Vol 59 (395) ◽

pp. 203-211 ◽

Cited By ~ 34

Author(s):

D. N. Bryon ◽

M. P. Atherton ◽

R. H. Hunter

Keyword(s):

Image Analysis ◽

Three Dimensional ◽

Three Dimensional Reconstruction ◽

Mineral Phase ◽

Thin Sections ◽

Thin Sectioning ◽

Potential Problems ◽

Dimensional Reconstruction ◽

Textural Development

AbstractTextural development of the felsic phases in two granodioritic rocks from the zoned Linga superunit of the Peruvian Coastal Batholith has been characterized using serial thin sectioning, image analysis and three-dimensional reconstruction. The study has shown how each mineral phase contributed to the texture during the formation and development of a contiguous crystal framework and during subsequent restriction, isolation and occlusion of the melt-filled porosity. The work highlights the important factors and potential problems in the use of serial thin sections and imaging in the analysis of complex polyphase rock textures.

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Structure of the native supercoiled flagellar hook as a universal joint

Nature Communications ◽

10.1038/s41467-019-13252-9 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 6

Author(s):

Takayuki Kato ◽

Fumiaki Makino ◽

Tomoko Miyata ◽

Péter Horváth ◽

Keiichi Namba

Keyword(s):

Image Analysis ◽

Basal Body ◽

Single Particle ◽

Particle Image ◽

Three Dimensional ◽

Universal Joint ◽

Density Map ◽

Smoke Ring ◽

Intersubunit Interactions ◽

Rotary Motor

AbstractThe Bacterial flagellar hook is a short supercoiled tubular structure made from a helical assembly of the hook protein FlgE. The hook acts as a universal joint that connects the flagellar basal body and filament, and smoothly transmits torque generated by the rotary motor to the helical filament propeller. In peritrichously flagellated bacteria, the hook allows the filaments to form a bundle behind the cell for swimming, and for the bundle to fall apart for tumbling. Here we report a native supercoiled hook structure at 3.6 Å resolution by cryoEM single particle image analysis of the polyhook. The atomic model built into the three-dimensional (3D) density map reveals the changes in subunit conformation and intersubunit interactions that occur upon compression and extension of the 11 protofilaments during their smoke ring-like rotation. These observations reveal how the hook functions as a dynamic molecular universal joint with high bending flexibility and twisting rigidity.

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