scholarly journals Replication of Colicin E1 Plasmid DNA in Minicells from a Unique Replication Initiation Site

1974 ◽  
Vol 71 (6) ◽  
pp. 2256-2259 ◽  
Author(s):  
J. Inselburg
Keyword(s):  
Plasmid Dna ◽  
Initiation Site ◽  
Replication Initiation ◽  
Colicin E1

scholarly journals Origin of replication of colicin E1 plasmid DNA.

1977 ◽  
Vol 74 (5) ◽  
pp. 1865-1869 ◽  
Author(s):  
J. I. Tomizawa ◽  
H. Ohmori ◽  
R. E. Bird
Keyword(s):  
Plasmid Dna ◽  
Origin Of Replication ◽  
Colicin E1

scholarly journals Determination of human DNA replication origin position and efficiency reveals principles of initiation zone organisation

2021 ◽  
Author(s):  
Guillaume Guilbaud ◽  
Pierre Murat ◽  
Helen S Wilkes ◽  
Leticia Koch Lerner ◽  
Julian Sale ◽  
...  
Keyword(s):  
Dna Replication ◽  
Replication Origin ◽  
Gradient Centrifugation ◽  
Initiation Site ◽  
Efficiency Score ◽  
Replication Initiation ◽  
Equilibrium Density ◽  
Replication Origins ◽  
Origin Firing ◽  
Dna Replication Origin

Replication of the human genome initiates within broad zones of ~ 150 kb. The extent to which firing of individual DNA replication origins within initiation zones is spatially stochastic or localised at defined sites remains a matter of debate. A thorough characterisation of the dynamic activation of origins within initiation zones is hampered by the lack of a high-resolution map of both their position and efficiency. To address this shortcoming, we describe a modification of initiation site sequencing (ini-seq) based on density substitution. Newly-replicated DNA is rendered heavy-light (HL) by incorporation of BrdUTP, unreplicated DNA remaining light-light (LL). Replicated HL-DNA is separated from unreplicated LL-DNA by equilibrium density gradient centrifugation, then both fractions are subjected to massive parallel sequencing. This allows precise mapping of 23,905 replication origins simultaneously with an assignment of a replication initiation efficiency score to each. We show that origin firing within initiation zones is not randomly distributed. Rather, origins are arranged hierarchically with a set of very highly efficient origins marking zone boundaries. We propose that these origins explain much of the early firing activity arising within initiation zones, helping to unify the concept of replication initiation zones with the identification of discrete replication origin sites.

Two replication initiation sites on R-plasmid DNA

1975 ◽  
Vol 140 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Jorge H. Crosa ◽  
Linda K. Luttropp ◽  
Fred Heffron ◽  
Stanley Falkow
Keyword(s):  
Plasmid Dna ◽  
Replication Initiation

Formation of catenated molecules by replication of colicin E1 plasmid DNA in cell extracts

1976 ◽  
Vol 108 (3) ◽  
pp. 569-582 ◽  
Author(s):  
Yoshimasa Sakakibara ◽  
Kenji Suzuki ◽  
Jun-ichi Tomizawa
Keyword(s):  
Plasmid Dna ◽  
Colicin E1 ◽  
Cell Extracts

scholarly journals Mapping of replication initiation site inMycoplasma capricolumgenome by two-dimensional gel-electrophoretic analysis

1993 ◽  
Vol 21 (20) ◽  
pp. 4816-4823 ◽  
Author(s):  
Makoto Miyata ◽  
Ken-lchi Sano ◽  
Ryosuke Okada ◽  
Takashi Fukumura
Keyword(s):  
Electrophoretic Analysis ◽  
Initiation Site ◽  
Replication Initiation ◽  
Two Dimensional
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