scholarly journals Biphasic stimulation of polyamine biosynthesis in primary mouse kidney cells by infection with polyoma virus:uncoupling from DNA and rRNA synthesis.

1976 ◽  
Vol 73 (11) ◽  
pp. 4022-4026 ◽  
Author(s):  
D. A. Goldstein ◽  
O. Heby ◽  
L. J. Marton
Keyword(s):  
Mouse Kidney ◽  
Rrna Synthesis ◽  
Kidney Cells ◽  
Polyamine Biosynthesis ◽  
Primary Mouse ◽  
Stimulation Of

Expression of oncomodulin does not lead to the transformation or immortalization of mammalian cells in vitro

1989 ◽  
Vol 94 (3) ◽  
pp. 517-525
Author(s):  
A.M. Mes-Masson ◽  
S. Masson ◽  
D. Banville ◽  
L. Chalifour
Keyword(s):  
Mammalian Cells ◽  
Mouse Kidney ◽  
Kidney Cells ◽  
T Antigens ◽  
Primary Mouse ◽  
Specific Antisera ◽  
Growth Properties ◽  
Metallothionein Promoter ◽  
Specific Mrna

A recombinant plasmid (pMTONCO) containing the coding sequences for rat oncomodulin under the direction of the metallothionein promoter was constructed. pMTONCO was co-transfected with the pSV2-NEO plasmid into primary mouse kidney cells or Rat-1 cells using the calcium phosphate technique and stable transformants were isolated after selection with G418. Transcription from the metallothionein promoter was inducible with heavy metals and produced an oncomodulin-specific mRNA. The presence of oncomodulin protein in stable cell lines was verified by immunoprecipitation with specific antisera. While a plasmid encoding the polyomavirus T-antigens was able to prolong the life-span of primary mouse kidney cells in culture, no equivalent activity was noted when the pMTONCO plasmid was used to transfect primary cells. When expressed in Rat-1 cells, oncomodulin did not affect the growth properties of these cells, nor did it predispose cells to higher frequencies of oncogenic transformation to a viral oncogene. We conclude that oncomodulin is neither an immortalizing nor transforming agent in vitro.

Evidence that stimulation of 1,25(OH)2D3 production in primary cultures of mouse kidney cells by cyclic AMP requires new protein synthesis

2009 ◽  
Vol 2 (6) ◽  
pp. 517-524 ◽  
Author(s):  
Adel B. Korkor ◽  
Richard W. Gray ◽  
Helen L. Henry ◽  
Jack G. Kleinman ◽  
Samuel S. Blumenthal ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Cyclic Amp ◽  
Primary Cultures ◽  
Mouse Kidney ◽  
Kidney Cells ◽  
New Protein ◽  
Stimulation Of

Effect of sodium butyrate on induction of cellular and viral DNA syntheses in polyoma virus-infected mouse kidney cells.

1981 ◽  
Vol 38 (3) ◽  
pp. 973-981 ◽  
Author(s):  
E Wawra ◽  
E Pöckl ◽  
E Müllner ◽  
E Wintersberger
Keyword(s):  
Infected Mouse ◽  
Sodium Butyrate ◽  
Mouse Kidney ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Viral Dna

The C-terminal region of the hepatitis B virus X protein is required for its stimulation of HBV replication in primary mouse hepatocytes

2012 ◽  
Vol 165 (2) ◽  
pp. 170-178 ◽  
Author(s):  
Na Luo ◽  
Yuan Cai ◽  
Jun Zhang ◽  
Weixue Tang ◽  
Betty L. Slagle ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Terminal Region ◽  
X Protein ◽  
Hbv Replication ◽  
Primary Mouse Hepatocytes ◽  
Primary Mouse ◽  
B Virus ◽  
Mouse Hepatocytes ◽  
Stimulation Of

Cloning, characterization, and gene organization of K-Cl cotransporter from pig and human kidney and C. elegans

1998 ◽  
Vol 275 (4) ◽  
pp. F550-F564 ◽  
Author(s):  
Eli J. Holtzman ◽  
Sumit Kumar ◽  
Carol A. Faaland ◽  
Fern Warner ◽  
Paul J. Logue ◽  
...  
Keyword(s):  
Amino Acids ◽  
Human Kidney ◽  
Gene Organization ◽  
Kidney Cells ◽  
Hek 293 ◽  
Human Embryonic Kidney Cells ◽  
C Elegans ◽  
Transmembrane Segments ◽  
Extracellular Loops ◽  
Stimulation Of

We isolated and characterized the cDNAs for the human, pig, and Caenorhabditis elegansK-Cl cotransporters. The pig and human homologs are 94% identical and contain 1,085 and 1,086 amino acids, respectively. The deduced protein of the C. elegans K-Cl cotransporter clone (CE-KCC1) contains 1,003 amino acids. The mammalian K-Cl cotransporters share ∼45% similarity with CE-KCC1. Hydropathy analyses of the three clones indicate typical KCC topology patterns with 12 transmembrane segments, large extracellular loops between transmembrane domains 5 and 6 (unique to KCC), and large COOH-terminal domains. Human KCC1 is widely expressed among various tissues. This KCC1 gene spans 23 kb and is organized in 24 exons, whereas the CE-KCC1 gene spans 3.5 kb and contains 10 exons. Transiently and stably transfected human embryonic kidney cells (HEK-293) expressing the human, pig, and C. elegans K-Cl cotransporter fulfilled two (pig) or five (human and C. elegans) criteria for increased expression of the K-Cl cotransporter. The criteria employed were basal K-Cl cotransport; stimulation of cotransport by swelling, N-ethylmaleimide, staurosporine, and reduced cell Mg concentration; and secondary stimulation of Na-K-Cl cotransport.

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