scholarly journals Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

1978 ◽  
Vol 75 (8) ◽  
pp. 3737-3741 ◽  
Author(s):  
J. G. Sutcliffe
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Resistance Gene ◽  
Plasmid Pbr322 ◽  
Ampicillin Resistance ◽  
Ampicillin Resistance Gene ◽  
Coli Plasmid

scholarly journals Transfer of an ampicillin resistance gene between two Escherichia coli strains in the bowel microbiota of an infant treated with antibiotics

2007 ◽  
Vol 60 (5) ◽  
pp. 1142-1145 ◽  
Author(s):  
N. Karami ◽  
A. Martner ◽  
V. I. Enne ◽  
S. Swerkersson ◽  
I. Adlerberth ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Ampicillin Resistance ◽  
Ampicillin Resistance Gene

Local DNA Sequence Control of Deletion Formation in Escherichia coli Plasmid pBR322

Genetics ◽  
1987 ◽  
Vol 115 (1) ◽  
pp. 41-49
Author(s):  
Ujjala DasGupta ◽  
Kathleen Weston-Hafer ◽  
Douglas E Berg
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Specific Interactions ◽  
Plasmid Pbr322 ◽  
Direct Repeats ◽  
Ampicillin Resistance ◽  
Deletion Formation ◽  
Coli Plasmid ◽  
Insertion Mutations ◽  
Dna Processing

ABSTRACT The specificity of deletion formation was studied using tests involving reversion of palindromic insertion mutations. Insertions of a Tn5-related transposon at 13 sites in the ampicillin-resistance (amp) gene of plasmid pBR322 were shortened to a nested set of perfect palindromes, 22, 32 and 90 bp long. We monitored frequencies of reversion to Ampr, which is the result of deletion of the palindrome plus one copy of the flanking 9 bp direct repeats (which had been formed by transposition). Revertant frequencies were found to depend on the location and the sequence of the palindromic insert. Changing a 45-kb interrupted palindrome to a 22-bp perfect palindrome stimulated deletion formation by factors of from fourfold to 545-fold among the 13 sites, while elongation of the perfect palindrome from 22 to 90 bp stimulated deletion formation by factors of from eight- to 18,000-fold. We conclude that deletion formation is strongly affected by subtle features of DNA sequence or conformation, both inside and outside the deleted segment, and that these effects may reflect specific interactions of DNA processing proteins with template DNAs.

Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101

Gene ◽  
1983 ◽  
Vol 24 (2-3) ◽  
pp. 309-315 ◽  
Author(s):  
Christine A. Miller ◽  
William T. Tucker ◽  
Peter A. Meacock ◽  
Petter Gustaf sson ◽  
Stanley N. Cohen
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Coli Plasmid

A novel shuttle vector for Streptomyces spp. and Escherichia coli as a tool in site-directed mutagenesis

1992 ◽  
Vol 38 (4) ◽  
pp. 350-353 ◽  
Author(s):  
A. Moreau ◽  
F. W. Paradis ◽  
R. Morosoli ◽  
F. Shareck ◽  
D. Kluepfel
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Shuttle Vector ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Gene Encoding ◽  
Single Stranded Dna ◽  
E Coli ◽  
Streptomyces Spp ◽  
Ampicillin Resistance Gene

This paper describes the construction and utilization of a novel shuttle vector for Streptomyces spp. and Escherichia coli as a useful vector in site-directed mutagenesis. The shuttle vector pIAFS20 (6.7 kb) has the following features: a replicon for Streptomyces spp., isolated from plasmid pIJ702; the thiostrepton-resistance gene as a selective marker in Streptomyces; the ColE1 origin, allowing replication in E. coli; and the ampicillin-resistance gene as a selective markerin E. coli. Vector pIAFS20 also contains the phage fl intergenic region, which permits production of single-stranded DNA in E. coli after superinfection with helper phage M13K07. Moreover, the lac promoter is located in front of the multiple cloning sites cassette, allowing eventual expression of the cloned genes in E. coli. After mutagenesis and screeningof the mutants in E. coli, the plasmids can be readily used to transform Streptomyces spp. As a demonstration, a 3.2-kb DNA fragment containing the gene encoding the xylanase A from Streptomyces lividans 1326 was inserted into pIAFS20, and the promoter region of this gene served as a target for site-directed mutagenesis. The two deletions reported here confirm the efficiency of this new vector as a tool in mutagenesis. Key words: shuttle vector, single-stranded DNA, site-directed mutagenesis, Streptomyces spp., Escherichia coli.

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