scholarly journals Nonselective expression of simian virus 40 large tumor antigen fragments in mouse cells.

1982 ◽  
Vol 79 (6) ◽  
pp. 2064-2067 ◽  
Author(s):  
V. B. Reddy ◽  
S. S. Tevethia ◽  
M. J. Tevethia ◽  
S. M. Weissman
Keyword(s):  
Tumor Antigen ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Large Tumor ◽  
Large Tumor Antigen ◽  
Mouse Cells

Differential roles of heat shock protein 70 in the in vitro nuclear import of glucocorticoid receptor and simian virus 40 large tumor antigen

1994 ◽  
Vol 14 (8) ◽  
pp. 5088-5098
Author(s):  
J Yang ◽  
D B DeFranco
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Tumor Antigen ◽  
Nuclear Import ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Large Tumor ◽  
Large Tumor Antigen ◽  
Cell Cytosol

Nuclear import of glucocorticoid receptors (GRs) was analyzed in vitro with digitonin-permeabilized cells (S. A. Adam, R. Sterne-Marr, and L. Gerace, J. Cell Biol. 111:807-816, 1990). Indirect immunofluorescence methods were used to monitor the transport of GRs from rat hepatoma and fibroblast cell cytosol into HeLa nuclei. In vitro nuclear import of GRs was shown to be hormone dependent and to require ATP and incubation at ambient temperatures (i.e., 30 degrees C). Hormone-dependent dissociation of GR-bound proteins, such as the 90-kDa heat shock protein, hsp90, is part of an activation process that is obligatory for the expression of the receptor's DNA-binding activity. Inhibition of in vitro GR activation by Na2MoO4 blocked hormone-dependent nuclear import, demonstrating that receptor activation is required for nuclear import. The addition to GR-containing cytosol of antiserum directed against the cytosolic 70-kDa heat shock protein, hsp70, while effective in blocking the nuclear import of simian virus 40 large tumor antigen (SV40 TAg), did not affect hormone-dependent nuclear import of endogenous, full-length GRs or an exogenously added truncated GR protein (i.e., XGR556) that lacks a hormone-binding domain but possesses a constitutively active nuclear localization signal sequence (NLS). Depletion of hsp70 from HeLa cell cytosol did not affect the nuclear import of exogenously added XGR556 but led to inhibition of SV40 TAg nuclear import. Thus, two closely related NLSs, one contained within GRs and the other contained within SV40 TAg, are distinguished by their differential requirements for hsp70 in vitro.

scholarly journals Simian Virus 40 Large Tumor Antigen Modulates the Raf Signaling Pathway

2001 ◽  
Vol 276 (30) ◽  
pp. 27840-27845 ◽  
Author(s):  
Nicholas Grammatikakis ◽  
Katarzyna Jaronczyk ◽  
Aliki Siganou ◽  
Adina Vultur ◽  
Heather Lee Brownell ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Tumor Antigen ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Large Tumor ◽  
Large Tumor Antigen

scholarly journals Carboxyl-terminal mutants of the large tumor antigen of simian virus 40: a role for the early protein late in the lytic cycle.

1988 ◽  
Vol 85 (2) ◽  
pp. 354-358 ◽  
Author(s):  
K. Khalili ◽  
J. Brady ◽  
J. M. Pipas ◽  
S. L. Spence ◽  
M. Sadofsky ◽  
...  
Keyword(s):  
Tumor Antigen ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Lytic Cycle ◽  
Large Tumor ◽  
Large Tumor Antigen ◽  
Early Protein ◽  
Carboxyl Terminal

scholarly journals Specific in vitro adenylylation of the simian virus 40 large tumor antigen.

1984 ◽  
Vol 81 (21) ◽  
pp. 6574-6578 ◽  
Author(s):  
M. K. Bradley ◽  
J. Hudson ◽  
M. S. Villanueva ◽  
D. M. Livingston
Keyword(s):  
Tumor Antigen ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Large Tumor ◽  
Large Tumor Antigen

scholarly journals Human mesotheliomas contain the simian virus-40 regulatory region and large tumor antigen DNA sequences

1998 ◽  
Vol 116 (5) ◽  
pp. 854-859 ◽  
Author(s):  
Harvey I. Pass ◽  
Jessica S. Donington ◽  
Peter Wu ◽  
Paola Rizzo ◽  
Michael Nishimura ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Tumor Antigen ◽  
Regulatory Region ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Large Tumor ◽  
Large Tumor Antigen

scholarly journals In vitro simian virus 40 large tumor antigen expression correlates with differential immune responses following DNA immunization

Virology ◽  
2005 ◽  
Vol 332 (1) ◽  
pp. 28-37 ◽  
Author(s):  
Devin B. Lowe ◽  
Michael H. Shearer ◽  
James A. Tarbox ◽  
Hyun Seok Kang ◽  
Cynthia A. Jumper ◽  
...  
Keyword(s):  
Immune Responses ◽  
Tumor Antigen ◽  
Simian Virus 40 ◽  
Antigen Expression ◽  
Simian Virus ◽  
Dna Immunization ◽  
Large Tumor ◽  
Large Tumor Antigen ◽  
Tumor Antigen Expression
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