scholarly journals Tunicamycin inhibits ganglioside biosynthesis in rat liver Golgi apparatus by blocking sugar nucleotide transport across the membrane vesicles.

1983 ◽  
Vol 80 (23) ◽  
pp. 7075-7079 ◽  
Author(s):  
H. K. Yusuf ◽  
G. Pohlentz ◽  
K. Sandhoff
Keyword(s):  
Golgi Apparatus ◽  
Rat Liver ◽  
Membrane Vesicles ◽  
Sugar Nucleotide ◽  
Ganglioside Biosynthesis

scholarly journals Ganglioside Biosynthesis in Golgi Apparatus of Rat Liver. Stimulation by Phosphatidylglycerol and Inhibition by Tunicamycin

1983 ◽  
Vol 134 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Harun K. M. YUSUF ◽  
Gottfried POHLENTZ ◽  
Gunter SCHWARZMANN ◽  
Konrad SANDHOFF
Keyword(s):  
Golgi Apparatus ◽  
Rat Liver ◽  
Ganglioside Biosynthesis

scholarly journals Subcellular biosynthesis and transport of gangliosides formed from exogenous lactosylceramide in rat liver

1990 ◽  
Vol 266 (2) ◽  
pp. 363-369 ◽  
Author(s):  
M Trinchera ◽  
R Ghidoni
Keyword(s):  
Plasma Membrane ◽  
Golgi Apparatus ◽  
Rat Liver ◽  
Organic Phase ◽  
Time Course ◽  
Plasma Membranes ◽  
Subcellular Fractionation ◽  
Ganglioside Biosynthesis ◽  
The Individual ◽  
Liposomal Dispersion

In order to clarify the mechanisms of ganglioside biosynthesis and transport we intravenously administered a liposomal dispersion of radiolabelled lactosylceramide (LacCer) to rats and then followed the time course of the individual gangliosides which became radioactive in the Golgi-apparatus and plasma-membrane fractions prepared from the liver. After administration of radiolabelled LacCer the liver retained a substantial amount of radioactivity, which was distributed among an organic phase (mainly residual LacCer), a fraction containing low-Mr substances (mainly 3H2O) and a ganglioside fraction. The hepatocytes were found to provide the bulk of gangliosides biosynthesized from exogenous LacCer. After subcellular fractionation, the total radioactive gangliosides increased in the Golgi apparatus up to 8 h, to then decrease and practically disappear at 24 h; in the plasma membranes they were progressively concentrated, accounting for high absolute values. Ganglioside patterns were greatly modified with time in both the Golgi apparatus and plasma membrane, but without significant differences between them. Biosynthesis in the Golgi apparatus and accumulation in the plasma membrane of each individual ganglioside followed a precursor-product relationship. The obtained results indicated that once a ganglioside is biosynthesized in the Golgi apparatus, it is in part made available for translocation to the plasma membrane, which rapidly occurs, and is in part retained in the Golgi apparatus, where it acts as a precursor for the biosynthesis of more glycosylated gangliosides.

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