scholarly journals Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

1996 ◽  
Vol 93 (18) ◽  
pp. 9670-9675 ◽  
Author(s):  
M. Suzuki ◽  
D. Baskin ◽  
L. Hood ◽  
L. A. Loeb
Keyword(s):  
Crystal Structure ◽  
Dna Polymerase ◽  
Random Mutagenesis ◽  
Dna Polymerase I ◽  
Thermus Aquaticus ◽  
Polymerase I

DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo

DNA Repair ◽  
2018 ◽  
Vol 64 ◽  
pp. 59-67 ◽  
Author(s):  
Kang-Yi Su ◽  
Liang-In Lin ◽  
Steven D. Goodman ◽  
Rong-Syuan Yen ◽  
Cho-Yuan Wu ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Exonuclease Activity ◽  
Repair Pathway ◽  
Dna Polymerase I ◽  
Endonuclease V ◽  
Polymerase I

Significance of DNA polymerase I in in vivo processing of clustered DNA damage

2013 ◽  
Vol 749 (1-2) ◽  
pp. 9-15 ◽  
Author(s):  
Naoya Shikazono ◽  
Ken Akamatsu ◽  
Momoko Takahashi ◽  
Miho Noguchi ◽  
Ayumi Urushibara ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Polymerase I ◽  
Clustered Dna Damage

Enhanced ribonucleotide incorporation by an O-helix mutant of Thermus aquaticus DNA polymerase I

2001 ◽  
Vol 485 (3) ◽  
pp. 197-207 ◽  
Author(s):  
Masanori Ogawa ◽  
Aki Tosaka ◽  
Yasutomo Ito ◽  
Shonen Yoshida ◽  
Motoshi Suzuki
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Thermus Aquaticus ◽  
Polymerase I

scholarly journals DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae

1989 ◽  
Vol 9 (2) ◽  
pp. 365-376
Author(s):  
M E Budd ◽  
K D Wittrup ◽  
J E Bailey ◽  
J L Campbell
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Dna Polymerase ◽  
Temperature Sensitive ◽  
Dna Polymerase I ◽  
X Ray ◽  
Temperature Sensitive Mutants ◽  
Polymerase I ◽  
Dna Polymerase Ii

We have used a set of seven temperature-sensitive mutants in the DNA polymerase I gene of Saccharomyces cerevisiae to investigate the role of DNA polymerase I in various aspects of DNA synthesis in vivo. Previously, we showed that DNA polymerase I is required for mitotic DNA replication. Here we extend our studies to several stages of meiosis and repair of X-ray-induced damage. We find that sporulation is blocked in all of the DNA polymerase temperature-sensitive mutants and that premeiotic DNA replication does not occur. Commitment to meiotic recombination is only 2% of wild-type levels. Thus, DNA polymerase I is essential for these steps. However, repair of X-ray-induced single-strand breaks is not defective in the DNA polymerase temperature-sensitive mutants, and DNA polymerase I is therefore not essential for repair of such lesions. These results suggest that DNA polymerase II or III or both, the two other nuclear yeast DNA polymerases for which roles have not yet been established, carry out repair in the absence of DNA polymerase I, but that DNA polymerase II and III cannot compensate for loss of DNA polymerase I in meiotic replication and recombination. These results do not, however, rule out essential roles for DNA polymerase II or III or both in addition to that for DNA polymerase I.

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